Amino Acids

, Volume 32, Issue 4, pp 467–477 | Cite as

Effects of resistance training and protein plus amino acid supplementation on muscle anabolism, mass, and strength

  • D. S. Willoughby
  • J. R. Stout
  • C. D. Wilborn


This study examined 10 wks of resistance training and the ingestion of supplemental protein and amino acids on muscle performance and markers of muscle anabolism. Nineteen untrained males were randomly assigned to supplement groups containing either 20 g protein (14 g whey and casein protein, 6 g free amino acids) or 20 g dextrose placebo ingested 1 h before and after exercise for a total of 40 g/d. Participants exercised 4 times/wk using 3 sets of 6–8 repetitions at 85–90% of the one repetition maximum. Data were analyzed with two-way ANOVA (p < 0.05). The protein supplement resulted in greater increases in total body mass, fat-free mass, thigh mass, muscle strength, serum IGF-1, IGF-1 mRNA, MHC I and IIa expression, and myofibrillar protein. Ten-wks of resistance training with 20 g protein and amino acids ingested 1 h before and after exercise is more effective than carbohydrate placebo in up-regulating markers of muscle protein synthesis and anabolism along with subsequent improvements in muscle performance.

Keywords: Resistance training – Insulin – Myosin heavy chain – IGF-1 – Amino acids 


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • D. S. Willoughby
    • 1
    • 2
    • 3
  • J. R. Stout
    • 4
  • C. D. Wilborn
    • 1
    • 2
  1. 1.Exercise and Biochemical Nutrition LaboratoryBaylor UniversityWacoU.S.A.
  2. 2.Exercise Nutrition and Resistance Training Research UnitBaylor UniversityWacoU.S.A.
  3. 3.Baylor Biomedical Science InstituteBaylor UniversityWacoU.S.A.
  4. 4.Department of Health and Exercise ScienceUniversity of OklahomaNormanU.S.A.

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