Skip to main content
SpringerLink
Log in

Decreases in taurine levels induced by β-alanine treatment did not affect the susceptibility of tissues to lipid peroxidation

  • Published:
Amino Acids Aims and scope Submit manuscript

Summary.

We aimed to investigate the effect of decreased taurine levels on endogenous and induced lipid peroxide levels in liver, brain, heart and erythrocytes as well as prooxidant and antioxidant balance in the liver of rats administered β-alanine (3%, w/v) in drinking water for 1 month to decrease taurine levels of tissues. This treatment caused significant decreases in taurine levels of liver (86%), brain (36%) and heart (15%). We found that endogenous and ascorbic acid-, NADPH- and cumene hydroperoxide-induced malondialdehyde (MDA) levels did not change in the liver, brain and heart homogenates following β-alanine treatment. Also, H2O2-induced MDA levels remained unchanged in erythrocytes. In addition, we did not observe any changes in levels of MDA, diene conjugates, glutathione, α-tocopherol, ascorbic acid and the activities of superoxide dismutase, glutathione peroxidase and glutathione transferase in the liver. According to this, buffering or sequestering capacity of tissues to exogenous stimuli was not influenced by reduced taurine levels in tissues of rats.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • SN Allo L Bagby SW Schaffer (1997) ArticleTitleTaurine depletion, a novel mechanism for cardioprotection from regional ischemia Am J Physiol 273 1956–1961

    Google Scholar 

  • J Balkan Ö Kanbağlı A Hatipoğlu M Küçük U Çevikbaş G Aykaç-Toker M Uysal (2002) ArticleTitleImproving effect of dietary taurine supplementation on the oxidative stress and lipid levels in the plasma, liver and aorta of rabbits fed on a high-cholesterol diet Biosci Biotechnol Biochem 66 1755–1758 Occurrence Handle12353642 Occurrence Handle1:CAS:528:DC%2BD38XmvFCrtLw%3D Occurrence Handle10.1271/bbb.66.1755

    Article  PubMed  CAS  Google Scholar 

  • J Balkan FH Parildar S Doğru-Abbasoğlu G Aykaç-Toker M Uysal (2005) ArticleTitleThe effect of taurine or betaine pretreatment on hepatotoxicity and prooxidant status induced by lipopolysaccharide treatment in the liver of rats Eur J Gastroenterol Hepatol 17 917–921 Occurrence Handle16093868 Occurrence Handle1:CAS:528:DC%2BD2MXnt1yktb4%3D Occurrence Handle10.1097/00042737-200509000-00006

    Article  PubMed  CAS  Google Scholar 

  • JD Bauer PG Ackermann G Toro (1974) Clinical laboratory methods C.V. Mosby St. Louis 97–98

    Google Scholar 

  • E Beutler O Duron BM Kelly (1963) ArticleTitleImproved method for the determination of blood glutathione J Lab Clin Med 61 882–888 Occurrence Handle13967893 Occurrence Handle1:CAS:528:DyaF3sXksV2rsbo%3D

    PubMed  CAS  Google Scholar 

  • JA Buege JD Aust (1978) ArticleTitleMicrosomal lipid peroxidation Methods Enzymol 52 302–310 Occurrence Handle672633 Occurrence Handle1:CAS:528:DyaE1MXltVajsw%3D%3D

    PubMed  CAS  Google Scholar 

  • L Chang J Xu F Yu J Zhao X Tang C Tang (2004) ArticleTitleTaurine protected myocardial mitochondria injury induced by hyperhomocysteinemia in rats Amino Acids 27 37–48 Occurrence Handle15309570 Occurrence Handle1:CAS:528:DC%2BD2cXmsVKktLc%3D Occurrence Handle10.1007/s00726-004-0096-2

    Article  PubMed  CAS  Google Scholar 

  • W Chen K Matuda N Nishimura H Yokogoshi (2004) ArticleTitleThe effect of taurine on cholesterol degradation in mice fed a high-cholesterol diet Life Sci 74 1889–1898 Occurrence Handle14761670 Occurrence Handle1:CAS:528:DC%2BD2cXptFCgsg%3D%3D Occurrence Handle10.1016/j.lfs.2003.08.041

    Article  PubMed  CAS  Google Scholar 

  • R Dawson S Liu B Eppler T Patterson (1999) ArticleTitleEffects of dietary taurine supplementation or deprivation in aged male Fischer 344 rats Mech Aging Dev 107 73–91 Occurrence Handle10197790 Occurrence Handle1:CAS:528:DyaK1MXnvVWntw%3D%3D Occurrence Handle10.1016/S0047-6374(98)00138-9

    Article  PubMed  CAS  Google Scholar 

  • R Dawson M Biasetti S Messina J Dominy (2002) ArticleTitleThe cytoprotective role of taurine in exercise-induced muscle injury Amino Acids 22 309–324 Occurrence Handle12107759 Occurrence Handle1:CAS:528:DC%2BD38XmvFCqt74%3D Occurrence Handle10.1007/s007260200017

    Article  PubMed  CAS  Google Scholar 

  • DI Desai (1984) ArticleTitleVitamin E analysis methods for animal tissues Methods Enzymol 105 138–147 Occurrence Handle6727662 Occurrence Handle1:CAS:528:DyaL2cXksFWisrw%3D

    PubMed  CAS  Google Scholar 

  • TPA Devasagayam U Tarachand (1987) ArticleTitleDecreased lipid peroxidation in the rat kidney during gestation Biochem Biophys Res Commun 145 134–138 Occurrence Handle3593335 Occurrence Handle1:CAS:528:DyaL2sXktlOhtb0%3D Occurrence Handle10.1016/0006-291X(87)91297-6

    Article  PubMed  CAS  Google Scholar 

  • B Eppler R Dawson (2000) ArticleTitleDietary taurine manipulations in aged male Fisher 344 rat tissue: taurine concentration, taurine biosynthesis, and oxidative markers Biochem Pharmacol 62 29–39 Occurrence Handle10.1016/S0006-2952(01)00647-5

    Article  Google Scholar 

  • F Erman J Balkan U Çevikbaş N Koçak-Toker M Uysal (2004) ArticleTitleBetaine or taurine administration prevents fibrosis and lipid peroxidation induced by rat liver by ethanol plus carbon tetrachloride intoxication Amino Acids 27 199–205 Occurrence Handle15338317 Occurrence Handle1:CAS:528:DC%2BD2cXoslGisLo%3D Occurrence Handle10.1007/s00726-004-0105-5

    Article  PubMed  CAS  Google Scholar 

  • O Golubnitschaja H Moenkemann K Kim MS Mozaffari (2003) ArticleTitleDNA damage and expression of checkpoint genes p21WAF1/CIP1 and 14-3-3 σ in taurine-deficient cardiomyocytes Biochem Pharmacol 66 511–517 Occurrence Handle12907251 Occurrence Handle1:CAS:528:DC%2BD3sXlvFygt7g%3D Occurrence Handle10.1016/S0006-2952(03)00285-5

    Article  PubMed  CAS  Google Scholar 

  • WH Habig WB Jacoby (1981) ArticleTitleAssays for differentation of glutathione S-transferases Methods Enzymol 77 398–405 Occurrence Handle7329316 Occurrence Handle1:CAS:528:DyaL38Xhtlynt7Y%3D

    PubMed  CAS  Google Scholar 

  • HH Hagar (2004) ArticleTitleThe protective effect of taurine agaist cyclosporine A-induced oxidative stress and hepatotoxicity in rats Toxicol Lett 151 335–343 Occurrence Handle15183458 Occurrence Handle1:CAS:528:DC%2BD2cXks1Wntbw%3D Occurrence Handle10.1016/j.toxlet.2004.03.002

    Article  PubMed  CAS  Google Scholar 

  • SH Hansen (2001) ArticleTitleThe role of taurine in diabetes and the development of diabetic complications Diabetes Metab Res Rev 17 330–346 Occurrence Handle11747139 Occurrence Handle1:CAS:528:DC%2BD3MXotlyksbg%3D Occurrence Handle10.1002/dmrr.229

    Article  PubMed  CAS  Google Scholar 

  • MDJ Kerai CJ Waterfield SH Kenyon DS Asker JA Timbrell (2001) ArticleTitleThe effect of taurine depletion by β-alanine treatment on the susceptibility to ethanol-induced hepatic dysfunction in rats Alcohol Alcoholism 36 29–38 Occurrence Handle1:CAS:528:DC%2BD3MXht1Shtbo%3D Occurrence Handle10.1093/alcalc/36.1.29

    Article  CAS  Google Scholar 

  • RA Lawrence RF Burk (1976) ArticleTitleGlutathione peroxidase activity in selenium deficient rat liver Biochem Biophys Res Commun 71 952–958 Occurrence Handle971321 Occurrence Handle1:CAS:528:DyaE28XlsVWls7s%3D Occurrence Handle10.1016/0006-291X(76)90747-6

    Article  PubMed  CAS  Google Scholar 

  • P Lu W Xu JA Sturman (1996) ArticleTitleDietary beta-alanine results in taurine depletion and cerebellar amage in adult cats J Neurosci Res 43 112–119 Occurrence Handle8838582 Occurrence Handle1:CAS:528:DyaK28Xos1KhtQ%3D%3D Occurrence Handle10.1002/jnr.490430115

    Article  PubMed  CAS  Google Scholar 

  • SA Mesina R Dawson (2000) ArticleTitleAttenuation of oxidative damage to DNA by taurine and taurine analogs Adv Exp Med Biol 483 355–367

    Google Scholar 

  • S Murakami Y Kondo K Tomisawa T Nagate (1999) ArticleTitlePrevention of atherosclerotic lesion development in mice by taurine Drug Exp Clin Res 25 227–234 Occurrence Handle1:CAS:528:DyaK1MXns1GjsbY%3D

    CAS  Google Scholar 

  • AA Mylorie H Collins C Umbles J Kyle (1986) ArticleTitleErythrocyte superoxide dismutase activity and other parameters of copper status in rats ingesting lead acetate Toxicol Appl Pharmacol 82 512–520 Occurrence Handle10.1016/0041-008X(86)90286-3

    Article  Google Scholar 

  • H Ohkawa N Ohishi K Yagi (1979) ArticleTitleAssay for lipid peroxidation in animal tissues by thiobarbituric acid reaction Anal Biochem 95 351–358 Occurrence Handle36810 Occurrence Handle1:CAS:528:DyaE1MXksFaisbk%3D Occurrence Handle10.1016/0003-2697(79)90738-3

    Article  PubMed  CAS  Google Scholar 

  • ST Omaye JD Turnbull HE Sauberlich (1979) ArticleTitleSelected methods for the determination ascorbic acid in animal cells, tissues and fluids Methods Enzymol 62 3–8 Occurrence Handle440112 Occurrence Handle1:CAS:528:DyaE1MXltF2ktbo%3D Occurrence Handle10.1016/0076-6879(79)62181-X

    Article  PubMed  CAS  Google Scholar 

  • G Pushpakiran K Mahalakshmi CV Anuradha (2004) ArticleTitleTaurine restores ethanol-induced depletion of antioxidants and attenuates oxidative stress in rat tissues Amino Acids 27 91–96 Occurrence Handle15309576 Occurrence Handle1:CAS:528:DC%2BD2cXmsVKkt7o%3D Occurrence Handle10.1007/s00726-004-0066-8

    Article  PubMed  CAS  Google Scholar 

  • S Schaffer J Azuma K Takahashi M Mozaffari (2003) ArticleTitleWhy is taurine cytoprotective? Adv Exp Med Biol 526 307–321 Occurrence Handle12908615 Occurrence Handle1:CAS:528:DC%2BD3sXnsVyhtLk%3D

    PubMed  CAS  Google Scholar 

  • SW Schaffer C Ballard-Croft J Azuma K Takahashi DG Kakhniashvili TE Jenkins (1998) ArticleTitleShape and size changes induced by taurine depletion in neonatal cardiomyocytes Amino Acids 15 135–142 Occurrence Handle9871493 Occurrence Handle1:CAS:528:DyaK1cXmvVGks7g%3D Occurrence Handle10.1007/BF01345286

    Article  PubMed  CAS  Google Scholar 

  • JE Shaffer JJ Kocsis (1981) Methods of reducing tissue taurine levels SW Schaffer SI Baskin JJ Kocsis (Eds) The effects of taurine on excitable tissues Spectrum Publications New York 219–229

    Google Scholar 

  • PK Smith RI Krohn GT Hermanson AK Mallia FH Gartner MD Provenzano EK Fujimoto NM Goeke BJ Olson DC Klenk (1985) ArticleTitleMeasurement of protein using bicinchoninic acid Anal Biochem 150 76–85 Occurrence Handle3843705 Occurrence Handle1:CAS:528:DyaL2MXlsFKksL0%3D Occurrence Handle10.1016/0003-2697(85)90442-7

    Article  PubMed  CAS  Google Scholar 

  • J Stocks L Offerman CB Modell TL Dormandy (1972) ArticleTitleThe susceptibility to autoxidation of human red blood cell lipids in health and disease Br J Haematol 23 713–724 Occurrence Handle4646825 Occurrence Handle1:STN:280:CSyD2snjtlM%3D

    PubMed  CAS  Google Scholar 

  • U Warskulat U Flögel C Jacoby HG Hartwig M Thewissen MW Merx A Molojavyi B Heller-Stilb J Schrader D Haussinger (2004) ArticleTitleTaurine transporter knockout depletes muscle taurine levels and results in severe skeletal muscle impairment but leaves cardiac function uncompromised FASEB J 18 577–579 Occurrence Handle14734644 Occurrence Handle1:CAS:528:DC%2BD2cXisVygs7g%3D

    PubMed  CAS  Google Scholar 

  • H Wu Y Jin J Wei H Jin D Sha J-Y Wu (2005) ArticleTitleMode of action of taurine as a neuroprotector Brain Res 1038 123–131 Occurrence Handle15757628 Occurrence Handle1:CAS:528:DC%2BD2MXitFCitrg%3D Occurrence Handle10.1016/j.brainres.2005.01.058

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, İstanbul Faculty of Medicine, İstanbul University, Çapa, Istanbul, Turkey

    H. Parıldar-Karpuzoğlu, S. Doğru-Abbasoğlu, J. Balkan, G. Aykaç-Toker & M. Uysal

Authors
  1. H. Parıldar-Karpuzoğlu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Doğru-Abbasoğlu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Balkan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Aykaç-Toker
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Uysal
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Parıldar-Karpuzoğlu, H., Doğru-Abbasoğlu, S., Balkan, J. et al. Decreases in taurine levels induced by β-alanine treatment did not affect the susceptibility of tissues to lipid peroxidation. Amino Acids 32, 115–119 (2007). https://doi.org/10.1007/s00726-005-0282-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00726-005-0282-x

Search

Navigation