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Alterations in neutrophil (PMN) free intracellular alpha-keto acid profiles and immune functions induced by L-alanyl-L-glutamine, arginine or taurine

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Summary.

The objective of this study was to determine the dose as well as duration of exposure-dependent effects of L-alanyl-L-glutamine, arginine or taurine on polymorphonuclear neutrophil (PMN) free α-keto acid profiles and, in a parallel study, on PMN immune functions. Exogenous L-alanyl-L-glutamine significantly increased PMN α-ketoglutarate, pyruvate PMN superoxide anion (O2 −) generation, hydrogen peroxide (H2O2) formation and released myeloperoxidase (MPO) activity. Arginine also led to significant increases in α-ketoglutarate, pyruvate, MPO release and H2O2 generation. Formation of O2 − on the other hand was decreased by arginine. Incubation with taurine resulted in lower intracellular pyruvate and α-ketobutyrate levels, decreased O2 − and H2O2 formation and a concomitant significantly increased MPO activity. We therefore believe that considerable changes in PMN free-α-keto-acid profiles, induced for example by L-alanyl-L-glutamine, arginine or taurine, may be one of the determinants in cell nutrition that considerably modulates the immunological competence of PMN.

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References

  • G Agam A Gutman (1972) ArticleTitleSynthesis of glycogen in leucocytes from various precursors. Rev Eur Etud Clin Biol 17 650–656 Occurrence Handle4346546 Occurrence Handle1:CAS:528:DyaE38XltlOrtro%3D

    PubMed  CAS  Google Scholar 

  • S Albers J Wernerman P Stehle E Vinnars P Fürst (1988) ArticleTitleAvailability of amino acids supplied intravenously in healthy man as synthetic dipeptides: kinetic evaluation of L-alanyl-L-glutamine and glycyl-L-tyrosine. Clin Sci (Lond) 75 463–468 Occurrence Handle1:CAS:528:DyaL1MXit1Ojsw%3D%3D

    CAS  Google Scholar 

  • W Alvarez S Mobarhan (2003) ArticleTitleFinding a place for immunonutrition. Nutr Rev 61 214–218 Occurrence Handle12903831

    PubMed  Google Scholar 

  • MS Ardawi EA Newsholme (1982) ArticleTitleMaximum activities of some enzymes of glycolysis, the tricarboxylic acid cycle and ketone-body and glutamine utilization pathways in lymphocytes of the rat. Biochem J 208 743–748 Occurrence Handle7165729 Occurrence Handle1:CAS:528:DyaL3sXitFegs7o%3D

    PubMed  CAS  Google Scholar 

  • V Bansal JB Ochoa (2003) ArticleTitleArginine availability, arginase, and the immune response. Curr Opin Clin Nutr Metab Care 6 223–228 Occurrence Handle12589193 Occurrence Handle1:CAS:528:DC%2BD3sXislejsr8%3D Occurrence Handle10.1097/00075197-200303000-00012

    Article  PubMed  CAS  Google Scholar 

  • A Barbul (1990) ArticleTitleArginine and immune function. Nutrition 6 53–58 Occurrence Handle2135757 Occurrence Handle1:CAS:528:DyaK3cXhtlOqsbk%3D

    PubMed  CAS  Google Scholar 

  • F Belfiore V Borzi LL Vecchio E Napoli AM Rabuazzo (1975) ArticleTitleEnzyme activities of NADPH-forming metabolic pathways in normal and leukemic leukocytes. Clin Chem 21 880–883 Occurrence Handle236846 Occurrence Handle1:CAS:528:DyaE2MXkslKksbw%3D

    PubMed  CAS  Google Scholar 

  • Bender DA (1985) Amino acid metabolism, 2nd edn., Chichester New York Brisbane Toronto Singapore

  • D Bihari (2002) ArticleTitleImmunonutrition in the critically ill. JPEN J Parenter Enteral Nutr 26 67–69 Occurrence Handle11833755 Occurrence Handle10.1177/014860710202600167

    Article  PubMed  Google Scholar 

  • MC Blanc C Moinard A Beziel S Darquy L Cynober JP De Bandt (2005) ArticleTitleArginine and glutamine availability and macrophage functions in the obese insulin-resistant Zucker rat. J Cell Physiol 202 153–159 Occurrence Handle15389544 Occurrence Handle1:CAS:528:DC%2BD2cXhtVOlsb%2FJ Occurrence Handle10.1002/jcp.20092

    Article  PubMed  CAS  Google Scholar 

  • RC Blantz K Munger (2002) ArticleTitleRole of nitric oxide in inflammatory conditions. Nephron 90 373–378 Occurrence Handle11961394 Occurrence Handle10.1159/000054723

    Article  PubMed  Google Scholar 

  • M Board S Humm EA Newsholme (1990) ArticleTitleMaximum activities of key enzymes of glycolysis, glutaminolysis, pentose phosphate pathway and tricarboxylic acid cycle in normal, neoplastic and suppressed cells. Biochem J 265 503–509 Occurrence Handle2302181 Occurrence Handle1:CAS:528:DyaK3cXhtFKmt7c%3D

    PubMed  CAS  Google Scholar 

  • K Brand W Fekl J von Hintzenstern K Langer P Luppa C Schoerner (1989) ArticleTitleMetabolism of glutamine in lymphocytes. Metabolism 38 29–33 Occurrence Handle2569663 Occurrence Handle1:CAS:528:DyaL1MXltlWmsro%3D Occurrence Handle10.1016/0026-0495(89)90136-4

    Article  PubMed  CAS  Google Scholar 

  • ND Burg MH Pillinger (2001) ArticleTitleThe neutrophil: function and regulation in innate and humoral immunity. Clin Immunol 99 7–17 Occurrence Handle11286537 Occurrence Handle1:CAS:528:DC%2BD3MXjslSnu7Y%3D Occurrence Handle10.1006/clim.2001.5007

    Article  PubMed  CAS  Google Scholar 

  • PC Calder (2003) ArticleTitleImmunonutrition. BMJ 327 117–118 Occurrence Handle12869428

    PubMed  Google Scholar 

  • L Castell (2003) ArticleTitleGlutamine supplementation in vitro and in vivo, in exercise and in immunodepression. Sports Med 33 323–345 Occurrence Handle12696982

    PubMed  Google Scholar 

  • J Cedergren P Follin T Forslund M Lindmark T Sundqvist T Skogh (2003) ArticleTitleInducible nitric oxide synthase (NOS II) is constitutive in human neutrophils. APMIS 111 963–968 Occurrence Handle14616549 Occurrence Handle1:CAS:528:DC%2BD3sXps12lsbg%3D Occurrence Handle10.1034/j.1600-0463.2003.1111008.x

    Article  PubMed  CAS  Google Scholar 

  • JG Chen SA Kempson (1995) ArticleTitleOsmoregulation of neutral amino acid transport. Proc Soc Exp Biol Med 210 1–6 Occurrence Handle7675792 Occurrence Handle1:CAS:528:DyaK2MXot1Khtbo%3D

    PubMed  CAS  Google Scholar 

  • C Cuisinier DW Michotte RK Verbeeck JR Poortmans R Ward X Sturbois M Francaux (2002) ArticleTitleRole of taurine in osmoregulation during endurance exercise. Eur J Appl Physiol 87 489–495 Occurrence Handle12355187 Occurrence Handle1:CAS:528:DC%2BD38XntlOmtbk%3D Occurrence Handle10.1007/s00421-002-0679-0

    Article  PubMed  CAS  Google Scholar 

  • R Curi P Newsholme EA Newsholme (1986) ArticleTitleIntracellular distribution of some enzymes of the glutamine utilisation pathway in rat lymphocytes. Biochem Biophys Res Commun 138 318–322 Occurrence Handle3741415 Occurrence Handle1:CAS:528:DyaL28XkvFGisLs%3D Occurrence Handle10.1016/0006-291X(86)90282-2

    Article  PubMed  CAS  Google Scholar 

  • R Curi P Newsholme TC Pithon-Curi M Pires-de-Melo C Garcia PI Homem-de-Bittencourt Junior AR Guimaraes (1999) ArticleTitleMetabolic fate of glutamine in lymphocytes, macrophages and neutrophils. Braz J Med Biol Res 32 15–21 Occurrence Handle10347763 Occurrence Handle1:CAS:528:DyaK1MXhtFymu7k%3D Occurrence Handle10.1590/S0100-879X1999000100002

    Article  PubMed  CAS  Google Scholar 

  • TC Curi MP De Melo RB De Azevedo R Curi (1997a) ArticleTitleGlutamine utilization by rat neutrophils. Biochem Soc Trans 25 249–251

    Google Scholar 

  • TC Curi MP De Melo RB De Azevedo TM Zorn R Curi (1997b) ArticleTitleGlutamine utilization by rat neutrophils: presence of phosphate-dependent glutaminase. Am J Physiol 273 C1124–C1129 Occurrence Handle1:CAS:528:DyaK2sXnt1Srtr0%3D

    CAS  Google Scholar 

  • C Dahlgren A Karlsson (1999) ArticleTitleRespiratory burst in human neutrophils. J Immunol Methods 232 3–14 Occurrence Handle10618505 Occurrence Handle1:CAS:528:DC%2BD3cXotVeh Occurrence Handle10.1016/S0022-1759(99)00146-5

    Article  PubMed  CAS  Google Scholar 

  • A Davidson (2004) ArticleTitleThe pharmacological effects of novel nutrients on the immune system. Nurs Times 100 62–63 Occurrence Handle15151012

    PubMed  Google Scholar 

  • R Denno JD Rounds R Faris LB Holejko DW Wilmore (1996) ArticleTitleGlutamine-enriched total parenteral nutrition enhances plasma glutathione in the resting state. J Surg Res 61 35–38 Occurrence Handle8769939 Occurrence Handle1:CAS:528:DyaK28Xhslyls7o%3D Occurrence Handle10.1006/jsre.1996.0077

    Article  PubMed  CAS  Google Scholar 

  • P Eggleton R Gargan D Fisher (1989) ArticleTitleRapid method for the isolation of neutrophils in high yield without the use of dextran or density gradient polymers. J Immunol Methods 121 105–113 Occurrence Handle2474025 Occurrence Handle1:STN:280:DyaL1Mzisl2nsw%3D%3D Occurrence Handle10.1016/0022-1759(89)90425-0

    Article  PubMed  CAS  Google Scholar 

  • D Evoy MD Lieberman TJ Fahey TJ SuffixIII JM Daly (1998) ArticleTitleImmunonutrition: the role of arginine. Nutrition 14 611–617 Occurrence Handle9684265 Occurrence Handle1:CAS:528:DyaK1cXlt1emtrw%3D Occurrence Handle10.1016/S0899-9007(98)00005-7

    Article  PubMed  CAS  Google Scholar 

  • M Farriol Y Venereo J Rossello P Gomez R Palao X Orta T Segovia-Silvestre (2002) ArticleTitleEffects of taurine on polymorphonuclear phagocytosis activity in burned patients. Amino Acids 23 441–445 Occurrence Handle12436213 Occurrence Handle1:CAS:528:DC%2BD38XoslOrtbc%3D Occurrence Handle10.1007/s00726-002-0209-8

    Article  PubMed  CAS  Google Scholar 

  • U Fauth W Heinrichs I Puente-Gonzalez M Halmagyi (1990) ArticleTitleMaximale Umsatzraten an Enzymen der Glykolyse und des Zitratzyklus von separierten Granulozyten in der postoperativen Phase. [Maximal turnover rates of glycolysis enzymes and of the citrate cycle of separated granulocytes in the postoperative period]. Infusionstherapie 17 178–183 Occurrence Handle2210862 Occurrence Handle1:STN:280:DyaK3M%2Fgsl2ntA%3D%3D

    PubMed  CAS  Google Scholar 

  • U Fauth T Schlechtriemen W Heinrichs I Puente-Gonzalez M Halmagyi (1993) ArticleTitleThe measurement of enzyme activities in the resting human polymorphonuclear leukocyte – critical estimate of a method. Eur J Clin Chem Clin Biochem 31 5–16 Occurrence Handle7679932 Occurrence Handle1:CAS:528:DyaK3sXhs1ekt7c%3D

    PubMed  CAS  Google Scholar 

  • J Frei A Aellig P Nessi (1975) ArticleTitleEnzyme system and coenzymes involved in the energy metabolism of leukocytes. Function and metabolism of polymorphonuclear neutrophils. Ann Biol Clin (Paris) 33 459–464 Occurrence Handle1:CAS:528:DyaE28Xhs1aisbg%3D

    CAS  Google Scholar 

  • P Fürst (2000) ArticleTitleConditionally indispensable amino acids (glutamine, cyst(e)ine, tyrosine, arginine, ornithine, taurine) in enteral feeding and the dipeptide concept. Nestle Nutr Workshop Ser Clin Perform Programme 3 199–217 Occurrence Handle11490607

    PubMed  Google Scholar 

  • P Fürst P Stehle (1993) ArticleTitleThe potential use of parenteral dipeptides in clinical nutrition. Nutr Clin Pract 8 106–114 Occurrence Handle8289757

    PubMed  Google Scholar 

  • P Fürst S Albers P Stehle (1990a) ArticleTitleDipeptides in clinical nutrition. Proc Nutr Soc 49 343–359

    Google Scholar 

  • P Fürst S Albers P Stehle (1990b) ArticleTitleGlutamine-containing dipeptides in parenteral nutrition. JPEN J Parenter Enteral Nutr 14 118–124

    Google Scholar 

  • P Fürst K Pogan M Hummel B Herzog P Stehle (1997a) ArticleTitleDesign of parenteral synthetic dipeptides for clinical nutrition: in vitro and in vivo utilization. Ann Nutr Metab 41 10–21

    Google Scholar 

  • P Fürst K Pogan P Stehle (1997b) ArticleTitleGlutamine dipeptides in clinical nutrition. Nutrition 13 731–737

    Google Scholar 

  • Gabrilovich D (1999) The neutrophils: new outlook for old cells. Imperial college press, London

  • C Garcia TC Pithon-Curi de Lourdes Firm dM Pires P Newsholme R Curi (1999) ArticleTitleEffects of adrenaline on glucose and glutamine metabolism and superoxide production by rat neutrophils. Clin Sci (Lond) 96 549–555 Occurrence Handle1:CAS:528:DyaK1MXktFyqtLc%3D

    CAS  Google Scholar 

  • RF Grimble (2001) ArticleTitleNutritional modulation of immune function. Proc Nutr Soc 60 389–397 Occurrence Handle11681814 Occurrence Handle1:CAS:528:DC%2BD3MXmslygsr8%3D Occurrence Handle10.1079/PNS2001102

    Article  PubMed  CAS  Google Scholar 

  • RF Grimble GK Grimble (1998) ArticleTitleImmunonutrition: role of sulfur amino acids, related amino acids, and polyamines. Nutrition 14 605–610 Occurrence Handle9684264 Occurrence Handle1:CAS:528:DyaK1cXlt1emtr8%3D

    PubMed  CAS  Google Scholar 

  • H Grimm A Kraus (2001) ArticleTitleImmunonutrition. Langenbecks Arch Surg 386 369–376 Occurrence Handle11685569 Occurrence Handle1:STN:280:DC%2BD3MngvVKhtw%3D%3D

    PubMed  CAS  Google Scholar 

  • W Hubl W Druml K Langer H Lochs (1989) ArticleTitleInfluence of molecular structure and plasma hydrolysis on the metabolism of glutamine-containing dipeptides in humans. Metabolism 38 59–62 Occurrence Handle2761422 Occurrence Handle1:STN:280:DyaL1MzktlOiuw%3D%3D Occurrence Handle10.1016/0026-0495(89)90143-1

    Article  PubMed  CAS  Google Scholar 

  • E Jacobson G Kurzawaski S Tustanowski (1986) ArticleTitleSynthesis and uptake of taurine by isolated human granulocytes. Folia Histochem Cytobiol 24 179–185 Occurrence Handle3770243 Occurrence Handle1:CAS:528:DyaL2sXhs1ahtQ%3D%3D

    PubMed  CAS  Google Scholar 

  • S Kausalya J Nath (1998) ArticleTitleInteractive role of nitric oxide and superoxide anion in neutrophil-mediated endothelial cell injury. J Leukoc Biol 64 185–191 Occurrence Handle9715257 Occurrence Handle1:CAS:528:DyaK1cXltFymu7k%3D

    PubMed  CAS  Google Scholar 

  • T Kobayashi S Tsunawaki H Seguchi (2001) ArticleTitleEvaluation of the process for superoxide production by NADPH oxidase in human neutrophils: evidence for cytoplasmic origin of superoxide. Redox Rep 6 27–36 Occurrence Handle11333112 Occurrence Handle1:CAS:528:DC%2BD3MXjsFSmur0%3D Occurrence Handle10.1179/135100001101536003

    Article  PubMed  CAS  Google Scholar 

  • W Krumholz C Demel S Jung G Meuthen G Hempelmann (1993) ArticleTitleThe influence of intravenous anaesthetics on polymorphonuclear leukocyte function. Can J Anaesth 40 770–774 Occurrence Handle8403161 Occurrence Handle1:STN:280:DyaK2c%2Fgs1ylsw%3D%3D Occurrence Handle10.1007/BF03009774

    Article  PubMed  CAS  Google Scholar 

  • W Krumholz C Demel S Jung G Meuthen J Knecht G Hempelmann (1995) ArticleTitleThe effects of thiopentone, etomidate, ketamine and midazolam on several bactericidal functions of polymorphonuclear leucocytes in vitro. Eur J Anaesthesiol 12 141–146 Occurrence Handle7781633 Occurrence Handle1:STN:280:DyaK2MzgtVyitw%3D%3D

    PubMed  CAS  Google Scholar 

  • H Liu RM Pope (2004) ArticleTitlePhagocytes: mechanisms of inflammation and tissue destruction. Rheum Dis Clin North Am 30 19–39 Occurrence Handle15061567

    PubMed  Google Scholar 

  • M Luciak K Trznadel (1983) ArticleTitleActivity of various lysosomal enzymes of neutrophils in the initial period of hemodialysis in patients with chronic uremia. Pol Tyg Lek 38 877–880 Occurrence Handle6361714 Occurrence Handle1:STN:280:DyaL2c%2FpsFantg%3D%3D

    PubMed  CAS  Google Scholar 

  • LA Marquez HB Dunford (1994) ArticleTitleChlorination of taurine by myeloperoxidase. Kinetic evidence for an enzyme-bound intermediate. J Biol Chem 269 7950–7956 Occurrence Handle8132515 Occurrence Handle1:CAS:528:DyaK2cXjtFyiu7o%3D

    PubMed  CAS  Google Scholar 

  • M Masuda K Horisaka T Koeda (1984a) ArticleTitleRole of taurine in neutrophil function. Nippon Yakurigaku Zasshi 84 283–292 Occurrence Handle1:CAS:528:DyaL2cXlsV2lsLs%3D

    CAS  Google Scholar 

  • M Masuda K Horisaka T Koeda (1984b) ArticleTitleInfluences of taurine on functions of rat neutrophils. Jpn J Pharmacol 34 116–118 Occurrence Handle1:CAS:528:DyaL2cXms1Cltg%3D%3D

    CAS  Google Scholar 

  • DM McLoughlin PP Stapleton FJ Bloomfield (1991) ArticleTitleInfluence of taurine and a substituted taurine on the respiratory burst pathway in the inflammatory response. Biochem Soc Trans 19 73–78 Occurrence Handle1645321 Occurrence Handle1:CAS:528:DyaK3MXhsFKrs7w%3D

    PubMed  CAS  Google Scholar 

  • J Metcoff (1986) ArticleTitleIntracellular amino acid levels as predictors of protein synthesis. J Am Coll Nutr 5 107–120 Occurrence Handle3722628 Occurrence Handle1:CAS:528:DyaL28XkvFGit78%3D

    PubMed  CAS  Google Scholar 

  • Metcoff J, Pederson J, Gable J, Llach F (1987) Protein synthesis, cellular amino acids, and energy levels in CAPD patients. Kidney Int [Suppl 22]: 136–144

  • J Metcoff G Distler R Weber T Graser K Scharer P Fürst D Schönberg (1988) ArticleTitleRelation of amino acids, energy levels and protein synthesis in chronic renal disease. Child Nephrol Urol 9 153–159 Occurrence Handle3252964

    PubMed  Google Scholar 

  • J Metcoff P Fürst K Scharer G Distler R Weber J Mangold TA Graser G Pfaff D Schonberg (1989) ArticleTitleEnergy production, intracellular amino acid pools, and protein synthesis in chronic renal disease. J Am Coll Nutr 8 271–284 Occurrence Handle2674253 Occurrence Handle1:STN:280:DyaL1MzotFWgtw%3D%3D

    PubMed  CAS  Google Scholar 

  • HJ Mitzkat K Wiegrefe U Meyer (1972) ArticleTitleEnzyme patterns of the energy-linked metabolism in blood cells of human diabetics. Horm Metab Res 4 107–110 Occurrence Handle5028216 Occurrence Handle1:STN:280:DyaE387ovFCltw%3D%3D

    PubMed  CAS  Google Scholar 

  • F Mizuho H Mori S Deguchi Y Ogawa T Hori (1996) ArticleTitleAspartate aminotransferase (AST) levels in human periodontium-derived cells. J Periodontol 67 733–736 Occurrence Handle8866310 Occurrence Handle1:CAS:528:DyaK28XlvVWjsbY%3D

    PubMed  CAS  Google Scholar 

  • Moffat-FLJ T Han ZM Li MD Peck W Jy YS Ahn AJ Chu LY Bourguignon (1996) ArticleTitleSupplemental L-arginine HCl augments bacterial phagocytosis in human polymorphonuclear leukocytes. J Cell Physiol 168 26–33

    Google Scholar 

  • C Moinard B Chauveau S Walrand C Felgines J Chassagne F Caldefie LA Cynober MP Vasson (1999) ArticleTitlePhagocyte functions in stressed rats: comparison of modulation by glutamine, arginine and ornithine 2-oxoglutarate. Clin Sci (Colch) 97 59–65 Occurrence Handle1:CAS:528:DyaK1MXltFyjurw%3D Occurrence Handle10.1042/CS19980418

    Article  CAS  Google Scholar 

  • C Moinard F Caldefie S Walrand C Felgines MP Vasson L Cynober (2000) ArticleTitleInvolvement of glutamine, arginine, and polyamines in the action of ornithine alpha-ketoglutarate on macrophage functions in stressed rats. J Leukoc Biol 67 834–840 Occurrence Handle10857856 Occurrence Handle1:CAS:528:DC%2BD3cXktFWmsbs%3D

    PubMed  CAS  Google Scholar 

  • C Moinard F Caldefie-Chezet S Walrand MP Vasson L Cynober (2002a) ArticleTitleEvidence that glutamine modulates respiratory burst in stressed rat polymorphonuclear cells through its metabolism into arginine. Br J Nutr 88 689–695 Occurrence Handle1:CAS:528:DC%2BD3sXktFKhsA%3D%3D

    CAS  Google Scholar 

  • C Moinard F Caldefie S Walrand A Tridon J Chassagne MP Vasson L Cynober (2002b) ArticleTitleEffects of ornithine 2-oxoglutarate on neutrophils in stressed rats: evidence for the involvement of nitric oxide and polyamines. Clin Sci (Lond) 102 287–295 Occurrence Handle1:CAS:528:DC%2BD38XislSnurw%3D

    CAS  Google Scholar 

  • JC Montejo A Zarazaga J Lopez-Martinez G Urrutia M Roque AL Blesa S Celaya R Conejero C Galban dL Garcia T Grau A Mesejo C Ortiz-Leyba M Planas J Ordonez FJ Jimenez (2003) ArticleTitleImmunonutrition in the intensive care unit. A systematic review and consensus statement. Clin Nutr 22 221–233 Occurrence Handle12765660

    PubMed  Google Scholar 

  • MS Mulligan AB Lentsch PA Ward (1998) ArticleTitle In vivo recruitment of neutrophils: consistent requirements for L-arginine and variable requirements for complement and adhesion molecules. Inflammation 22 327–339 Occurrence Handle9604719 Occurrence Handle1:CAS:528:DyaK1cXjsVSmu74%3D

    PubMed  CAS  Google Scholar 

  • M Munder F Mollinedo J Calafat J Canchado C Gil-Lamaignere JM Fuentes C Luckner G Doschko G Soler K Eichmann FM Muller AD Ho M Goerner M Modolell (2005) ArticleTitleArginase I is constitutively expressed in human granulocytes and participates in fungicidal activity. Blood 105 2549–2556 Occurrence Handle15546957 Occurrence Handle1:CAS:528:DC%2BD2MXisFSkur4%3D Occurrence Handle10.1182/blood-2004-07-2521

    Article  PubMed  CAS  Google Scholar 

  • J Mühling ME Campos A Sablotzki M Krüll MG Dehne J Gonter S Weiss M Fuchs G Hempelman (2002a) ArticleTitleEffects of propofol and taurine on intracellular free amino acid profiles and immune function markers in neutrophils in vitro. Clin Chem Lab Med 40 111–121

    Google Scholar 

  • J Mühling M Fuchs A Sablotzki C Fleck M Krüll MG Dehne J Gonter S Weiss J Engel G Hempelman (2002b) ArticleTitleEffects of arginine, L-alanyl-L-glutamine or taurine on neutrophil (PMN) free amino acid profiles and immune functions in vitro. Amino Acids 22 39–53

    Google Scholar 

  • J Mühling M Fuchs ME Campos J Gonter JM Engel A Sablotzki T Menges S Weiss MG Dehne M Krüll G Hempelmann (2003) ArticleTitleQuantitative determination of free intracellular alpha-keto acids in neutrophils. J Chromatogr B Analyt Technol Biomed Life Sci 789 383–392 Occurrence Handle12742129

    PubMed  Google Scholar 

  • J Mühling M Fuchs M Campos J Gonter A Sablotzki J Engel ID Welters M Wolff R Matejec MG Dehne T Menges M Krüll G Hempelmann (2004) ArticleTitleEffects of ornithine on neutrophil (PMN) free amino acid and alpha-keto acid profiles and immune functions in vitro. Amino Acids 27 313–319 Occurrence Handle15538644

    PubMed  Google Scholar 

  • P Newsholme (2001) ArticleTitleWhy is L-glutamine metabolism important to cells of the immune system in health, postinjury, surgery or infection? J Nutr 131 2515–2522

    Google Scholar 

  • EA Newsholme B Crabtree MS Ardawi (1985) ArticleTitleThe role of high rates of glycolysis and glutamine utilization in rapidly dividing cells. Biosci Rep 5 393–400 Occurrence Handle3896338 Occurrence Handle1:CAS:528:DyaL2MXltVans7c%3D Occurrence Handle10.1007/BF01116556

    Article  PubMed  CAS  Google Scholar 

  • EA Newsholme P Newsholme R Curi (1987) ArticleTitleThe role of the citric acid cycle in cells of the immune system and its importance in sepsis, trauma and burns. Biochem Soc Symp 54 145–162 Occurrence Handle3332991 Occurrence Handle1:CAS:528:DyaL1cXmt1Omur4%3D

    PubMed  CAS  Google Scholar 

  • P Newsholme MM Lima J Procopio TC Pithon-Curi SQ Doi RB Bazotte R Curi (2003a) ArticleTitleGlutamine and glutamate as vital metabolites. Braz J Med Biol Res 36 153–163 Occurrence Handle1:CAS:528:DC%2BD3sXitVyksrc%3D Occurrence Handle10.1590/S0100-879X2003000200002

    Article  CAS  Google Scholar 

  • P Newsholme J Procopio MM Lima TC Pithon-Curi R Curi (2003b) ArticleTitleGlutamine and glutamate-their central role in cell metabolism and function. Cell Biochem Funct 21 1–9 Occurrence Handle1:CAS:528:DC%2BD3sXhs1Snsb0%3D Occurrence Handle10.1002/cbf.1003

    Article  CAS  Google Scholar 

  • Y O’Dowd P Newsholme (1997) ArticleTitleEvidence for the involvement of glutamine in nitric oxide (NO) production by immunostimulated neutrophils. Biochem Soc Trans 25 403–405

    Google Scholar 

  • TC Pithon-Curi AC Levada LR Lopes SQ Doi R Curi (2002a) ArticleTitleGlutamine plays a role in superoxide production and the expression of p47phox, p22phox and gp91phox in rat neutrophils. Clin Sci (Lond) 103 403–408 Occurrence Handle1:CAS:528:DC%2BD38XotVWltLY%3D

    CAS  Google Scholar 

  • TC Pithon-Curi AG Trezena W Tavares-Lima R Curi (2002b) ArticleTitleEvidence that glutamine is involved in neutrophil function. Cell Biochem Funct 20 81–86 Occurrence Handle1:CAS:528:DC%2BD38XjvV2mur8%3D Occurrence Handle10.1002/cbf.954

    Article  CAS  Google Scholar 

  • TC Pithon-Curi MP De Melo R Curi (2004) ArticleTitleGlucose and glutamine utilization by rat lymphocytes, monocytes and neutrophils in culture: a comparative study. Cell Biochem Funct 22 321–326 Occurrence Handle15338472 Occurrence Handle1:CAS:528:DC%2BD2cXnvVeit7k%3D Occurrence Handle10.1002/cbf.1109

    Article  PubMed  CAS  Google Scholar 

  • DW Porter SA Walker WG Martin P Lee W Kaczmarczyk (1991) ArticleTitleTaurine uptake in chicken leukocytes and erythrocytes. Comp Biochem Physiol A 98 305–309 Occurrence Handle1673894 Occurrence Handle1:STN:280:DyaK3M3itlygtQ%3D%3D Occurrence Handle10.1016/0300-9629(91)90537-M

    Article  PubMed  CAS  Google Scholar 

  • P Raschke P Massoudy BF Becker (1995) ArticleTitleTaurine protects the heart from neutrophil-induced reperfusion injury. Free Radic Biol Med 19 461–471 Occurrence Handle7590395 Occurrence Handle1:CAS:528:DyaK2MXnsVynur8%3D Occurrence Handle10.1016/0891-5849(95)00044-X

    Article  PubMed  CAS  Google Scholar 

  • HP Redmond PP Stapleton P Neary HD Bouchier (1998) ArticleTitleImmunonutrition: the role of taurine. Nutrition 14 599–604 Occurrence Handle9684263 Occurrence Handle1:CAS:528:DyaK1cXlt1emtr4%3D Occurrence Handle10.1016/S0899-9007(98)00097-5

    Article  PubMed  CAS  Google Scholar 

  • Rick W (1977) Klinische Chemie und Mikroskopie. Springer Berlin Heidelberg New York

  • J Rodenas T Carbonell MT Mitjavila (1996) ArticleTitleConditions to study nitric oxide generation by polymorphonuclear cells from an inflammatory exudate in rats. Arch Biochem Biophys 327 292–294 Occurrence Handle8619617 Occurrence Handle1:CAS:528:DyaK28Xhs1ajsbo%3D

    PubMed  CAS  Google Scholar 

  • J Rodenas MT Mitjavila T Carbonell (1998) ArticleTitleNitric oxide inhibits superoxide production by inflammatory polymorphonuclear leukocytes. Am J Physiol 274 C827–C830 Occurrence Handle9530115 Occurrence Handle1:CAS:528:DyaK1cXitVCrsbg%3D

    PubMed  CAS  Google Scholar 

  • E Roth R Oehler N Manhart R Exner B Wessner E Strasser A Spittler (2002) ArticleTitleRegulative potential of glutamine – relation to glutathione metabolism. Nutrition 18 217–221 Occurrence Handle11882392 Occurrence Handle1:CAS:528:DC%2BD38Xhs1ylsL0%3D Occurrence Handle10.1016/S0899-9007(01)00797-3

    Article  PubMed  CAS  Google Scholar 

  • D Salvemini H Ischiropoulos S Cuzzocrea (2003) ArticleTitleRoles of nitric oxide and superoxide in inflammation. Methods Mol Biol 225 291–303 Occurrence Handle12769497 Occurrence Handle1:CAS:528:DC%2BD3sXjsValsro%3D

    PubMed  CAS  Google Scholar 

  • J Satriano (2004) ArticleTitleArginine pathways and the inflammatory response: interregulation of nitric oxide and polyamines: review article. Amino Acids 26 321–329 Occurrence Handle15290337 Occurrence Handle1:CAS:528:DC%2BD2cXmtVWktLk%3D Occurrence Handle10.1007/s00726-004-0078-4

    Article  PubMed  CAS  Google Scholar 

  • S Schaffer K Takahashi J Azuma (2000) ArticleTitleRole of osmoregulation in the actions of taurine. Amino Acids 19 527–546 Occurrence Handle11140357 Occurrence Handle1:CAS:528:DC%2BD3MXotFSqsA%3D%3D Occurrence Handle10.1007/s007260070004

    Article  PubMed  CAS  Google Scholar 

  • S Schaffer J Azuma K Takahashi M Mozaffari (2003) ArticleTitleWhy is taurine cytoprotective? Adv Exp Med Biol 526 307–321 Occurrence Handle12908615 Occurrence Handle1:CAS:528:DC%2BD3sXnsVyhtLk%3D

    PubMed  CAS  Google Scholar 

  • RM Schapira JH Wiessner JF Morrisey UA Almagro LD Nelin (1998) ArticleTitleL-arginine uptake and metabolism by lung macrophages and neutrophils following intratracheal instillation of silica in vivo. Am J Respir Cell Mol Biol 19 308–315 Occurrence Handle9698604 Occurrence Handle1:CAS:528:DyaK1cXlsVeqs7g%3D

    PubMed  CAS  Google Scholar 

  • GB Schuller-Levis E Park (2003) ArticleTitleTaurine: new implications for an old amino acid. FEMS Microbiol Lett 226 195–202 Occurrence Handle14553911 Occurrence Handle1:CAS:528:DC%2BD3sXnvFWitbk%3D Occurrence Handle10.1016/S0378-1097(03)00611-6

    Article  PubMed  CAS  Google Scholar 

  • GB Schuller-Levis E Park (2004) ArticleTitleTaurine and its chloramine: modulators of immunity. Neurochem Res 29 117–126 Occurrence Handle14992270 Occurrence Handle1:CAS:528:DC%2BD2cXlsFyj Occurrence Handle10.1023/B:NERE.0000010440.37629.17

    Article  PubMed  CAS  Google Scholar 

  • GB Schuller-Levis JA Sturman (1992) ArticleTitle“Activation” of alveolar leukocytes isolated from cats fed taurine-free diets. Adv Exp Med Biol 315 83–90 Occurrence Handle1324596 Occurrence Handle1:CAS:528:DyaK3sXjsl2qsg%3D%3D

    PubMed  CAS  Google Scholar 

  • G Schuller-Levis PD Mehta R Rudelli J Sturman (1990) ArticleTitleImmunologic consequences of taurine deficiency in cats. J Leukoc Biol 47 321–331 Occurrence Handle2319206 Occurrence Handle1:CAS:528:DyaK3cXitFGmsbg%3D

    PubMed  CAS  Google Scholar 

  • P Seth R Kumari M Dikshit RC Srimal (1994) ArticleTitleModulation of rat peripheral polymorphonuclear leukocyte response by nitric oxide and arginine. Blood 84 2741–2748 Occurrence Handle7919387 Occurrence Handle1:CAS:528:DyaK2MXht1Sqs7k%3D

    PubMed  CAS  Google Scholar 

  • R Singh S Gopalan A Sibal (2002) ArticleTitleImmunonutrition. Indian J Pediatr 69 417–419 Occurrence Handle12061676 Occurrence Handle1:STN:280:DC%2BD38zhvFSgtQ%3D%3D

    PubMed  CAS  Google Scholar 

  • M Son JI Ko WB Kim HK Kang BK Kim (1998) ArticleTitleTaurine can ameliorate inflammatory bowel disease in rats. Adv Exp Med Biol 442 291–298 Occurrence Handle9635044 Occurrence Handle1:CAS:528:DyaK1cXls1Wqsrw%3D

    PubMed  CAS  Google Scholar 

  • P Stapleton RP Charles HP Redmond DJ Bouchier-Hayes (1997) ArticleTitleTaurine and human nutrition. Clin Nutr 16 103–108 Occurrence Handle1:CAS:528:DyaK2sXlsFWksL4%3D Occurrence Handle16844580

    CAS  PubMed  Google Scholar 

  • PP Stapleton L O’Flaherty HP Redmond DJ Bouchier-Hayes (1998a) ArticleTitleHost defense – a role for the amino acid taurine? JPEN J Parenter Enteral Nutr 22 42–48 Occurrence Handle1:CAS:528:DC%2BD3cXlvFGgt7s%3D Occurrence Handle10.1177/014860719802200142

    Article  CAS  Google Scholar 

  • PP Stapleton HP Redmond DJ Bouchier-Hayes (1998b) ArticleTitleMyeloperoxidase (MPO) may mediate neutrophil adherence to the endothelium through upregulation of CD11B expression – an effect downregulated by taurine. Adv Exp Med Biol 442 183–192 Occurrence Handle1:CAS:528:DyaK1cXls1Wrtbs%3D

    CAS  Google Scholar 

  • JK Stechmiller B Childress T Porter (2004) ArticleTitleArginine immunonutrition in critically ill patients: a clinical dilemma. Am J Crit Care 13 17–23 Occurrence Handle14735643

    PubMed  Google Scholar 

  • P Stehle I Ratz P Fürst (1989) ArticleTitle In vivo utilization of intravenously supplied L-alanyl-L-glutamine in various tissues of the rat. Nutrition 5 411–415 Occurrence Handle2520335 Occurrence Handle1:CAS:528:DyaK3cXpslCjsg%3D%3D

    PubMed  CAS  Google Scholar 

  • P Stehle I Ratz P Fürst (1991) ArticleTitleWhole-body autoradiography in rats after intravenous administration of L-alanyl-L-[U-14C]glutamine. Ann Nutr Metab 35 213–220 Occurrence Handle1897901 Occurrence Handle1:CAS:528:DyaK3MXlvVWqtLg%3D Occurrence Handle10.1159/000177648

    Article  PubMed  CAS  Google Scholar 

  • RL Stjernholm NV Dimitrov LJ Pijanowski (1969) ArticleTitleCarbohydrate metabolism in leukocytes. IX. Citric acid cycle activity in human neutrophils. J Reticuloendothel Soc 6 194–201 Occurrence Handle5812418 Occurrence Handle1:CAS:528:DyaF1MXktFSqurY%3D

    PubMed  CAS  Google Scholar 

  • U Suchner KS Kuhn P Fürst (2000) ArticleTitleThe scientific basis of immunonutrition. Proc Nutr Soc 59 553–563 Occurrence Handle11115790 Occurrence Handle1:CAS:528:DC%2BD3MXhs1ertA%3D%3D

    PubMed  CAS  Google Scholar 

  • JD Walters DM Sorboro KJ Chapman (1992) ArticleTitlePolyamines enhance calcium mobilization in fMet-Leu-Phe-stimulated phagocytes. FEBS Lett 304 37–40 Occurrence Handle1319922 Occurrence Handle1:CAS:528:DyaK38Xlt12rsro%3D Occurrence Handle10.1016/0014-5793(92)80584-4

    Article  PubMed  CAS  Google Scholar 

  • T Washizu E Kuramoto M Abe T Sako T Arai (1998) ArticleTitleA comparison of the activities of certain enzymes related to energy metabolism in leukocytes in dogs and cats. Vet Res Commun 22 187–192 Occurrence Handle9618890 Occurrence Handle1:STN:280:DyaK1c3ot1KisA%3D%3D Occurrence Handle10.1023/A:1006073305479

    Article  PubMed  CAS  Google Scholar 

  • CJ Wiedermann B Sitte U Zilian N Reinisch H Beimpold G Finkenstedt H Braunsteiner (1993) ArticleTitleInhibition of superoxide anion release from circulating neutrophils by L-arginine in man. Clin Investig 71 985–989 Occurrence Handle8124056 Occurrence Handle1:STN:280:DyaK2c7mvFKisg%3D%3D Occurrence Handle10.1007/BF00180028

    Article  PubMed  CAS  Google Scholar 

  • HL Willems TF de Kort FJ Trijbels LA Monnens JH Veerkamp (1978) ArticleTitleDetermination of pyruvate oxidation rate and citric acid cycle activity in intact human leukocytes and fibroblasts. Clin Chem 24 200–203 Occurrence Handle627049 Occurrence Handle1:CAS:528:DyaE1cXhs1Gmt7k%3D

    PubMed  CAS  Google Scholar 

  • V Witko-Sarsat P Rieu B Descamps-Latscha P Lesavre L Halbwachs-Mecarelli (2000) ArticleTitleNeutrophils: molecules, functions and pathophysiological aspects. Lab Invest 80 617–653 Occurrence Handle10830774 Occurrence Handle1:CAS:528:DC%2BD3cXktFGjt7Y%3D

    PubMed  CAS  Google Scholar 

  • DM Wolfe PD Gatfield (1975) ArticleTitleLeukocyte urea cycle enzymes in hyperammonemia. Pediatr Res 9 531–535 Occurrence Handle1161340 Occurrence Handle1:CAS:528:DyaE2MXksFKnsrs%3D

    PubMed  CAS  Google Scholar 

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  1. Department of Anaesthesiology, Intensive Care Medicine, Pain Therapy, University Hospital, Justus Liebig University, Giessen, Germany

    J. Mühling, K. A. Nickolaus, M. Halabi, J. Engel, M. Wolff, R. Matejec, T. W. Langefeld, I. D. Welters, T. Menges, M. G. Dehne & G. Hempelmann

  2. Dr. Ing. Herbert Knauer GmbH, Berlin, Germany

    M. Fuchs

  3. Department of Internal Medicine/Infectious Diseases, Charité, Medical School of Humboldt University, Berlin, Germany

    M. Krüll

  4. Clinics of Anaesthesiology and Intensive Care Medicine, Martin Luther University, Halle-Wittenberg, Germany

    A. Sablotzki

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  1. J. Mühling
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Mühling, J., Nickolaus, K., Halabi, M. et al. Alterations in neutrophil (PMN) free intracellular alpha-keto acid profiles and immune functions induced by L-alanyl-L-glutamine, arginine or taurine. Amino Acids 29, 289–300 (2005). https://doi.org/10.1007/s00726-005-0223-8

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