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Amino Acids

, Volume 27, Issue 3–4, pp 261–268 | Cite as

Characteristics of basal taurine release in the rat striatum measured by microdialysis

  • S. Molchanova
  • S. S. Oja
  • P. Saransaari
Article

Summary.

Taurine is a sulfur-containing amino acid thought to be an osmoregulator, neurotransmitter or neuromodulator in the brain. Our objective was to establish how much taurine is released in the striatum and examine the mechanisms controlling extracellular taurine concentrations under resting conditions. The experiments were made on rats by microdialysis in vivo. Changes in taurine were compared with those in glutamate, glycine and the non-neuroactive amino acid threonine. Using the zero net flux approach we showed the extracellular concentration of taurine to be 25.2 ± 5.1 μM. Glutamate was increased by tetrodotoxin and decreased by Ca2+ omission, glycine and threonine were not affected and both treatments increased extracellular taurine. The basal taurine release was increased by the taurine transport inhibitor guanidinoethanesulfonate and reduced by the anion channel blocker 4-acetamido-4′-isothiocyanatostilbene-2,2′-disulfonic acid.

Keywords: Taurine – Glutamate – Glycine – Threonine – Zero net flux – Synaptic transmission 

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Copyright information

© Springer-Verlag/Wien 2004

Authors and Affiliations

  • S. Molchanova
    • 1
    • 2
  • S. S. Oja
    • 1
    • 3
  • P. Saransaari
    • 1
  1. 1.Brain Research Center, Medical School, University of TampereTampereFinland
  2. 2.I.M. Sechenov Institute of Evolutionary Physiology and BiochemistrySaint PetersburgRussia
  3. 3.Department of Clinical PhysiologyTampere University HospitalTampereFinland

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