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Amino Acids

, Volume 27, Issue 3–4, pp 339–344 | Cite as

Mass spectrometrical analysis of phosphoprotein enriched in astrocytes of 15 kDa in mouse hippocampi

  • J.-H. Shin
  • J.-M. Delabar
  • G. Lubec
Short Communication

Summary.

Phosphoprotein enriched in astrocytes of 15 kDa (PEA-15) is a small protein that was first identified as an abundant phosphoprotein in brain. PEA-15 was characterised so far at the immunochemical level and by a microsequencing attempt. In order to update characterisation of this important structure by advanced methodology unambiguously identifying proteins independent of antibody availability and specificity, we used a proteomic method for this purpose: Performing protein profiling in mouse hippocampi using two dimensional gel electrophoresis with subsequent mass spectrometrical (MS/MS) identification we detected this protein and demonstrate proteomic characterisation of PEA-15 (Q62048). This study enables further specific and unambiguous determination serving as an analytical tool.

Keywords: Astrocytic phosphoprotein PEA-15 – Two-dimensional gel electrophoresis – Hippocampus – Mouse 

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Copyright information

© Springer-Verlag/Wien 2004

Authors and Affiliations

  • J.-H. Shin
    • 1
  • J.-M. Delabar
    • 2
  • G. Lubec
    • 1
  1. 1.Department of PediatricsMedical University of ViennaViennaAustria
  2. 2.Department of BiochemistryUniversite 7ParisFrance

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