Summary.
We investigated whether L-arginine, used in heart preservation to limit endothelial damage, may influence the pool of amino acids during long term ischemia and reflow. Isolated isovolumic rat hearts (n = 23) were submitted to 8 h of hypothermic ischemia after cardioplegic arrest with the Centre de Résonance Magnétique Biologique et Médicale (CRMBM) solution with or without L-arginine (Arg and No Arg groups respectively). Hearts were freeze-clamped after ischemia (n = 11) or submitted to 60 min of reflow (n = 12) and freeze-clamped. Eight hearts were perfused aerobically for 20 min and freeze-clamped (No ischemia group). Addition of L-arginine to the CRMBM solution limited aspartate depletion and decreased lysine level at the end of ischemia. After reflow, L-arginine supplementation increased the pool of glutamate and arginine and limited the depletion of serine, asparagine, glycine and taurine. We conclude that adding L-arginine to the CRMBM cardioplegic solution during long term ischemia preserved the amino acids pool.
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Received March 3, 2002 Accepted May 16, 2002 Published online October 3, 2002
Acknowledgements This work was supported by the “Ministère de la Recherche et de la Technologie” (grant n° 91 C 0959), CNRS (UMR 6612), Assistance Publique-Hôpitaux de Marseille and Programme Hospitalier de Recherche Clinique (PHRC).
Authors' address: Monique Bernard, Ph.D., Centre de Résonance Magnétique Biologique et Médicale (CRMBM), UMR CNRS 6612, Faculté de Médecine, 27 Bd Jean Moulin, 13005 Marseille, France, Fax: 33 (4) 91 25 65 39, E-mail: monique.bernard@medecine.univ-mrs.fr
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Desrois, M., Sciaky, M., Lan, C. et al. Preservation of amino acids during long term ischemia and subsequent reflow with supplementation of L-arginine, the nitric oxide precursor, in the rat heart. Amino Acids 24, 141–148 (2003). https://doi.org/10.1007/s00726-002-0321-9
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DOI: https://doi.org/10.1007/s00726-002-0321-9