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New Disulfide-Linked Dinitroxides and the Kinetics of Their Reaction with Glutathione

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Abstract

The health of living systems depends on the proper balance of cellular oxidative and reductive processes (redox balance), which is reflected in the thiol-disulfide balance in cells. Glutathione is the predominant low-molecular-weight thiol in mammalian cells. Through its diverse actions as an antioxidant, as a detoxifier of xenobiotics, and as a regulator of protein function, among others, glutathione is vitally important to cell health. Spectroscopic probes that can act as sensors or reporters of glutathione are, therefore, useful. We report here the synthesis of three new disulfide-linked dinitroxides, whose EPR spectra change dramatically upon reaction with glutathione, and the characterization of the kinetics of their reaction with glutathione.

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Funding

Supported by the Nanobiology Research Fund of the University of Maryland Baltimore Foundation.

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Authors and Affiliations

  1. Center for Biomedical Engineering and Technology (BioMET), University of Maryland School of Medicine, Baltimore, USA

    Mitasha S. Palha, Eric A. Legenzov, Nathaniel D. A. Dirda & Joseph P. Y. Kao

  2. Department of Biochemistry, University of Maryland School of Medicine, Baltimore, USA

    Mitasha S. Palha

  3. Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, USA

    Gerald M. Rosen

  4. Department of Physiology, University of Maryland School of Medicine, Baltimore, USA

    Eric A. Legenzov & Joseph P. Y. Kao

Authors
  1. Mitasha S. Palha
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  2. Eric A. Legenzov
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  5. Joseph P. Y. Kao
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by MSP, EAL NDAD, GMR, and JPYK. The first draft of the manuscript was written by MSP and JPYK and all authors edited and commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Joseph P. Y. Kao.

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The authors declare no competing interests.

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The original online version of this article was revised: due to change in figure caption.

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Palha, M.S., Legenzov, E.A., Dirda, N.D.A. et al. New Disulfide-Linked Dinitroxides and the Kinetics of Their Reaction with Glutathione. Appl Magn Reson 52, 945–955 (2021). https://doi.org/10.1007/s00723-021-01332-3

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  • DOI: https://doi.org/10.1007/s00723-021-01332-3

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