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Protoplasma

, Volume 256, Issue 4, pp 951–969 | Cite as

Two grapevine metacaspase genes mediate ETI-like cell death in grapevine defence against infection of Plasmopara viticola

  • Peijie GongEmail author
  • Michael Riemann
  • Duan Dong
  • Nadja Stoeffler
  • Bernadette Gross
  • Armin Markel
  • Peter Nick
Original Article

Abstract

Metacaspase, as hypersensitive response (HR) executors, has been identified in many plant species. Previously, the entire gene family of metacaspase has been uncovered, but there are still questions that remain unclear regarding HR-regulating gene members. In this study, based on metacaspase expression during different grapevine genotypes interacting with Plasmopara viticola, we identified MC2 and MC5 as candidates involved in HR. We overexpressed both metacaspases as GFP fusions in tobacco BY-2 cells to address subcellular localization and cellular functions. We found MC2 located at the ER, while MC5 was nucleocytoplasmic. In these overexpressor lines, cell death elicited by the bacterial protein harpin, is significantly enhanced, indicating MC2 and MC5 mediated defence-related programmed cell death (PCD). This effect was mitigated, when the membrane-located NADPH oxidase was inhibited by the specific inhibitor diphenylene iodonium, or when cells were complemented with methyl jasmonate, a crucial signal of basal immunity. Both findings are consistent with a role of MC2 and MC5 in cell death-related immunity. Using a dual-luciferase reporter system in grapevine cells we demonstrated both MC2 and MC5 promoter alleles from V. rupestris were more responsive to harpin than those from V. vinifera cv ‘Müller-Thurgau’, while they were not induced by MeJA as signal linked with basal immunity. These findings support a model, where MC2 and MC5 act specifically as executors of the HR.

Keywords

Metacaspase Programmed cell death (PCD) Plant immunity Vitis rupestris Hypersensitive response (HR) 

Notes

Funding information

This study was kindly supported by a fellowship from the Chinese Scholarship Council to Peijie Gong, as well as a project fund from the BACCHUS Interreg project IV Oberrhein/Rhin supérieur.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Botanical Institute, Karlsruhe Institute of TechnologyKarlsruheGermany

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