Composition and functional property of photosynthetic pigments under circadian rhythm in the cyanobacterium Spirulina platensis
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Circadian rhythm is an important endogenous biological signal for sustainable growth and development of cyanobacteria in natural ecosystems. Circadian effects of photosynthetically active radiation (PAR), ultraviolet-A (UV-A) and ultraviolet-B (UV-B) radiations on pigment composition have been studied in the cyanobacterium Spirulina platensis under light (L)/dark (D) oscillation with a combination of 4/20, 8/16, 12/12, 16/8, 20/4 and 24/24 h time duration. Circadian exposure of PAR + UV-A (PA) and PAR + UV-A + UV-B (PAB) showed more than twofold decline in Chl a, total protein and phycocyanin (PC) in light phase and significant recovery was achieved in dark phase. The fluorescence emission wavelength of PC was shifted towards lower wavelengths in the light phase of PAB in comparison to P and PA whereas the same wavelength was retrieved in the dark phase. The production of free radicals was accelerated twofold in the light phase (24 h L) whereas the same was retrieved to the level of control during the dark phase. Oxidatively induced damage was alleviated by antioxidative enzymes such as catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and ascorbate peroxidase (APX) in the light phase (0–24-h L) whereas the dark phase showed significant inhibition of the same enzymes. Similar characteristic inhibition of free radicals and recovery of PC was observed inside cellular filament after circadian rhythm of 24/24 h (L/D). Circadian exposure of P, PA and PAB significantly altered the synthesis and recovery of pigments that could be crucial for optimization and sustainable production of photosynthetic products for human welfare.
KeywordsAntioxidative enzymes Circadian rhythm Free radicals Phycocyanin Ultraviolet radiation
Photosynthetically active radiation
Reactive oxygen species
We are thankful to the Interdisciplinary School of Life Sciences (ISLS), BHU, Varanasi, India, for providing access to the fluorescence microscopy facility.
V.K. Kannaujiya designed the experiments, evaluated the results and wrote the manuscript. D. Kumar performed the experiments and analyzed data. Richa and J. Pathak performed writing and editing of the manuscript. S. Sundaram critically analyzed the manuscript. R.P. Sinha supervised the experiments and critically analyzed the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Abràmoff MD, Magalhães PJ, Ram SJ (2004) Image processing with ImageJ. Biophoton Int 11:36–42Google Scholar
- Bhandari R, Sharma PK (2006) High-light-induced changes on photosynthesis, pigments, sugars, lipids and antioxidant enzymes in freshwater (Nostoc spongiaeforme) and marine (Phormidium corium) cyanobacteria. Photochem Photobiol 82(3):702–710. https://doi.org/10.1562/2005-09-20-RA-690 CrossRefPubMedGoogle Scholar
- Britton C, Mehley AC (1955) Assay of catalase and peroxidase. In: Colowick SP, Kalpan NO (eds) Method in enzymology. Academic, New York, pp 764–775Google Scholar
- Chis C, Druga B, Carmel AD, Chis I, Ardelean A, Sicora CI (2016) UV-B stress changes the electron flow on photosystem II complex in Synechococcus sp. PCC 7002. Rom. Biotechnol Lett 22:12142–12146Google Scholar
- Dere S, Günes T, Sivaci R (1998) Spectrophotometric determination of chlorophyll-A, B and total carotenoid contents of some algae species using different solvents. Turk J Bot 22:13–17Google Scholar
- Häder D-P, Williamson CE, Wängberg S, Rautio M, Rose KC, Gao K, Helbling EW, Sinha RP, Worrest R (2015) Effects of UV radiation on aquatic ecosystems and interactions with other environmental factors. Photochem Photobiol Sci 14(1):108–126. https://doi.org/10.1039/C4PP90035A CrossRefPubMedGoogle Scholar
- He Y-Y, Häder D-P (2002) UV-B-induced formation of reactive oxygen species and oxidative damage of the cyanobacterium Anabaena sp.: protective effects of ascorbic acid and N-acetyl-l-cysteine. J Photochem Photobiol B Bio 66(2):115–124. https://doi.org/10.1016/S1011-1344(02)00231-2 CrossRefGoogle Scholar
- Marwood CA, Greenberg BM (1996) Effect of supplementary UV-B radiation on chlorophyll synthesis and accumulation of photosystems during chloroplast development in Spirodela oligorrhiza. Photochem Photobiol 64(4):664–670. https://doi.org/10.1111/j.1751-1097.1996.tb03121.x CrossRefGoogle Scholar
- Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
- Pattanaik B, Roleda MY, Schumann R, Karsten U (2008) Isolate-specific effects of ultraviolet radiation on photosynthesis, growth and mycosporine-like amino acids in the microbial mat-forming cyanobacterium Microcoleus chthonoplastes. Planta 227(4):907–916. https://doi.org/10.1007/s00425-007-0666-0 CrossRefPubMedGoogle Scholar
- Polo LK, de Marthiellen LFR, Kreusch M, Pereira DT, Costa GB, Simioni C, Ouriques LC, Chow F, Ramlov F, Maraschin M, Bouzon ZL, Schmidt EC (2014) Photo acclimation responses of the brown macroalga Sargassum cymosum to the combined influence of UV radiation and salinity: cytochemical and ultrastructural organization and photosynthetic performance. Photochem Photobiol 90(3):560–573. https://doi.org/10.1111/php.12224 CrossRefPubMedGoogle Scholar
- Rastogi RP, Singh SP, Häder D-P, Sinha RP (2010) Detection of reactive oxygen species (ROS) by the oxidant-sensing probe 2′,7′-dichlorodihydrofluorescein diacetate in the cyanobacterium Anabaena variabilis PCC 7937. Biochem Biophys Res Commun 397(3):603–607. https://doi.org/10.1016/j.bbrc.2010.06.006 CrossRefPubMedGoogle Scholar
- Rastogi RP, Sonani RR, Madamwar D (2015) Effects of PAR and UV radiation on the structural and functional integrity of phycocyanin, phycoerythrin and allophycocyanin isolated from the marine cyanobacterium Lyngbya sp. A09DM. Photochem Photobiol 91(4):837–844. https://doi.org/10.1111/php.12449 CrossRefPubMedGoogle Scholar
- Richa, Kannaujiya VK, Kesheri M, Singh G, Sinha RP (2011) Biotechnological potentials of phycobiliproteins. Int J Pharma Bio Sci 2:446–454Google Scholar
- Sinha RP, Lebert M, Kumar A, Kumar HD, Häder D-P (1995) Spectroscopic and biochemical analyses of UV effects on phycobiliproteins of Anabaena sp. and Nostoc carmium. Bot Acta 108(2):87–92. https://doi.org/10.1111/j.1438-8677.1995.tb00836.x CrossRefGoogle Scholar
- Sinha RP, Gröniger A, Klisch M, Häder D-P (2002b) Ozone depletion and ultraviolet-B radiation: impacts on aquatic organism. Recent Res Devel Photochem Photobiol 6:95–106Google Scholar
- Sinha RP, Barbieri ES, Lebert M, Helbling EW, Häder D-P (2003a) Effects of solar radiation on phycobiliproteins of marine red algae. Trends Photochem Photobiol 10:149–157Google Scholar
- Sinha RP, Helbling EW, Häder D-P (2003b) Effects of solar radiation on photosynthetic quantum yield of a cyanobacterium Nostoc sp. Trends Photochem Photobiol 10:159–166Google Scholar
- Tripathi SN, Srivastava P (2001) Presence of stable active oxygen scavenging enzymes superoxide dismutase, ascorbate peroxidase and catalase in a desiccation-tolerant cyanobacterium Lyngbya arboricola under dry state. Curr Sci 81:197–200Google Scholar
- Young AJ (1991) The photoprotective role of carotenoids in higher plants. Physiol Plant 83(4):702–708. https://doi.org/10.1111/j.1399-3054.1991.tb02490.x CrossRefGoogle Scholar