Protoplasma

, Volume 255, Issue 3, pp 977–983 | Cite as

Jasmonic acid-induced NO activates MEK1/2 in regulating the metabolism of ascorbate and glutathione in maize leaves

Short Communication
First Online:
Received:
Accepted:
  • 83 Downloads

Abstract

This study investigated the relationship between MEK1/2 and nitric oxide (NO) in jasmonic acid (JA)-regulated metabolism of ascorbate and glutathione in maize leaves. The results showed that JA increased the activities of APX, GR, MDHAR, DHAR, GalLDH, and γ-ECS; the contents of AsA and GSH; and the production of NO. Above increases except for γ-ECS activity and NO production were all suppressed by pre-treatments with MEK1/2 inhibitors PD98059 and U0126. Above increases were all suppressed by pre-treatments with nitric oxide synthase (NOS) inhibitor l-NAME and NO scavenger cPTIO. The results of western blot showed that JA enhanced the phosphorylation level of MEK1/2. Pre-treatments with l-NAME and cPTIO suppressed JA-induced phosphorylation level of MEK1/2. Our results suggested that JA-induced NO activated MEK1/2 by increasing the phosphorylation level, which, in turn, resulted in the upregulation of ascorbate and glutathione metabolism in maize leaves.

Keywords

MEK1/2 NO Ascorbate Glutathione Maize 
This is a preview of subscription content, log in to check access

Notes

Compliance with ethical standards

Conflict of interest

The author declares that there is no conflict of interest.

References

  1. Ai L, Li ZH, Xie ZX, Tian XL, Eneji AE, Duan LS (2008) Coronatine alleviates polyethylene glycol-induced water stress in two rice (Oryza sativa L.) cultivars. J Agron Crop Sci 194:360–368CrossRefGoogle Scholar
  2. Alavi-Samani SM, Kachouei MA, Pirbalouti AG (2015) Growth, yield, chemical composition, and antioxidant activity of essential oils from two thyme species under foliar application of jasmonic acid and water deficit conditions. Hortic Environ Biotechnol 56:411–420CrossRefGoogle Scholar
  3. Bo H, Wang PT, Dong FC, Song CP (2005) The methyl jasmonate-induced H2O2 generation and relation to MAPK signal transduction system in Arabidopsis thaliana guard cells. Plant Physiol Commun 41:439–443Google Scholar
  4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefPubMedGoogle Scholar
  5. Brodersen P, Petersen M, Bjorn Nielsen H, Zhu S, Newman MA, Shokat KM, Rietz S, Parker J, Mundy J (2006) Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic acid/ethylene-dependent responses via EDS1 and PAD4. Plant J 47:532–546CrossRefPubMedGoogle Scholar
  6. Dalton DA, Russell SA, Hanus FJ, Pascoe GA, Evans HJ (1986) Enzymatic reactions of ascorbate and glutathione that prevent peroxide damage in soybean root nodules. Proc Natl Acad Sci U S A 83:3811–3815CrossRefPubMedPubMedCentralGoogle Scholar
  7. Dringen R (2000) Glutathione metabolism and oxidative stress in neurodegeneration. Eur J Biochem 267:4903CrossRefPubMedGoogle Scholar
  8. Grace SC, Logan BA (1996) Acclimation of foliar antioxidant systems to growth irradiance in three broad-leaved evergreen species. Plant Physiol 112:1631–1640CrossRefPubMedPubMedCentralGoogle Scholar
  9. Griffith OW (1980) Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem 106:207–212CrossRefPubMedGoogle Scholar
  10. Hu T, Hu Z, Zeng H, Qv X, Chen G (2015) Tomato lipoxygenase D involved in the biosynthesis of jasmonic acid and tolerance to abiotic and biotic stress in tomato. Plant Biotechnol Rep 9:37–45CrossRefGoogle Scholar
  11. Kampfenkel K, Van Montagu M, Inzè D (1995) Extraction and determination of ascorbate and dehydroascorbate from plant tissue. Anal Biochem 225:165–167CrossRefPubMedGoogle Scholar
  12. Miyake C, Asada K (1992) Thylakoid-bound ascorbate peroxidase in spinach chloroplasts and photoreduction of its primary oxidation product monodehydroascorbate radicals in thylakoids. Plant Cell Physiol 33:541–553Google Scholar
  13. Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
  14. Rüegsegger A, Brunold C (1992) Effect of cadmium on γ-glutamylcysteine synthesis in maize seedlings. Plant Physiol 99:428–433CrossRefPubMedPubMedCentralGoogle Scholar
  15. Shan C, Liang Z (2010) Jasmonic acid regulates ascorbate and glutathione metabolism in Agropyron cristatum leaves under water stress. Plant Sci 178:130–139CrossRefGoogle Scholar
  16. Shan C, Liang Z, Sun Y, Hao W, Han R (2011) The protein kinase MEK1/2 participates in the regulation of ascorbate and glutathione content by jasmonic acid in Agropyron cristatum leaves. J Plant Physiol 168:514–518CrossRefPubMedGoogle Scholar
  17. Shan C, He F, Xu G, Han R, Liang Z (2012) Nitric oxide is involved in the regulation of ascorbate and glutathione metabolism in Agropyron cristatum leaves under water stress. Biol Plant 56:187–191CrossRefGoogle Scholar
  18. Shan C, Zhou Y, Liu M (2015) Nitric oxide participates in the regulation of the ascorbate-glutathione cycle by exogenous jasmonic acid in the leaves of wheat seedlings under drought stress. Protoplasma 252:1–9CrossRefGoogle Scholar
  19. Singh VP, Singh S, Kumar J, Prasad SM (2015) Investigating the roles of ascorbate-glutathione cycle and thiol metabolism in arsenate tolerance in ridged Luffa seedlings. Protoplasma 252:1217–1229CrossRefPubMedGoogle Scholar
  20. Tabata K, Oba K, Suzuki K, Esaka M (2001) Generation and properties of ascorbic acid-deficient transgenic tobacco cells expressing antisense RNA of L-galactono-1,4-lactone dehydrogenase. Plant J 27:139–148CrossRefPubMedGoogle Scholar
  21. Takahashi F, Yoshida R, Ichimura K, Mizoguchi T, Seo S, Yonezawa M, Maruyama K, Yamaguchi-Shinozaki K, Shinozaki K (2007) The mitogen-activated protein kinase cascade MKK3–MPK6 is an important part of the jasmonate signal transduction pathway in Arabidopsis. Plant Cell 19:805–818CrossRefPubMedPubMedCentralGoogle Scholar
  22. Wheeler GL, Jones MA, Smirnoff N (1998) The biosynthetic pathway of vitamin C in higher plants. Nature 393:365–369CrossRefPubMedGoogle Scholar
  23. Zhang S, Klessig DF (1997) Salicylic acid activates a 48-kD MAP kinase in tobacco. Plant Cell 9:809–824CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Henan Institute of Science and TechnologyXinxiangChina
  2. 2.Collaborative Innovation Center of Modern Biological BreedingXinxiangChina

Personalised recommendations