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Protoplasma

, Volume 255, Issue 3, pp 977–983 | Cite as

Jasmonic acid-induced NO activates MEK1/2 in regulating the metabolism of ascorbate and glutathione in maize leaves

  • Changjuan Shan
  • Haili Sun
Short Communication
First Online:
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Accepted:

Abstract

This study investigated the relationship between MEK1/2 and nitric oxide (NO) in jasmonic acid (JA)-regulated metabolism of ascorbate and glutathione in maize leaves. The results showed that JA increased the activities of APX, GR, MDHAR, DHAR, GalLDH, and γ-ECS; the contents of AsA and GSH; and the production of NO. Above increases except for γ-ECS activity and NO production were all suppressed by pre-treatments with MEK1/2 inhibitors PD98059 and U0126. Above increases were all suppressed by pre-treatments with nitric oxide synthase (NOS) inhibitor l-NAME and NO scavenger cPTIO. The results of western blot showed that JA enhanced the phosphorylation level of MEK1/2. Pre-treatments with l-NAME and cPTIO suppressed JA-induced phosphorylation level of MEK1/2. Our results suggested that JA-induced NO activated MEK1/2 by increasing the phosphorylation level, which, in turn, resulted in the upregulation of ascorbate and glutathione metabolism in maize leaves.

Keywords

MEK1/2 NO Ascorbate Glutathione Maize 
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Notes

Funding information

This study was financially supported by Project of Supporting Young Backbone Teachers of Colleges and Universities in Henan province (2016GGJS-108).

Compliance with ethical standards

Conflict of interest

The author declares that there is no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Henan Institute of Science and TechnologyXinxiangChina
  2. 2.Collaborative Innovation Center of Modern Biological BreedingXinxiangChina

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