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Electrochemical determination of indole-3-acetic acid and indole-3-butyric acid using HPLC with carbon felt detector

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Abstract

Method for the determination of two auxins, indole-3-acetic acid and indole-3-butyric acid, is developed using HPLC with electrochemical detection. As the working electrode, recently developed carbon felt detector with high conversion degree was employed. Optimum conditions, i.e. separation on 125-4 Lichrospher 100, RP-18 column with mobile phase consisting of buffer pH 4.0 and methanol (40:60, v/v) and detection at potential + 1.5 V, allow to obtain linear concentration dependences in the concentration range from 1 to 100 µmol dm−3, with determination limits of 1.1 µmol dm−3 and 1.8 µmol dm−3 for indole-3-acetic and -butyric acid, respectively. Applicability of the method was verified by the determination of both analytes in rooting preparation; the results were in agreement with the reference method, although not with the values declared by the manufacturer.

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Acknowledgements

This research was financially supported by The Czech Science Foundation (project GA17-03868S).

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Authors and Affiliations

  1. UNESCO Laboratory of Environmental Electrochemistry, Department of Analytical Chemistry, Faculty of Science, Charles University, Albertov 6, 128 43, Prague 2, Czech Republic

    Hana Dejmkova

  2. Federal University of Rio Grande do Sul, Av. Paulo Gama, 110, Farroupilha, Porto Alegre, RS, 90040-060, Brazil

    Marjorie de Araújo Daniel

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  1. Hana Dejmkova
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  2. Marjorie de Araújo Daniel
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Correspondence to Hana Dejmkova.

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Dejmkova, H., de Araújo Daniel, M. Electrochemical determination of indole-3-acetic acid and indole-3-butyric acid using HPLC with carbon felt detector. Monatsh Chem 150, 439–442 (2019). https://doi.org/10.1007/s00706-019-2375-3

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  • DOI: https://doi.org/10.1007/s00706-019-2375-3

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