Molecularly imprinted polymer as stationary phase for HPLC separation of phenylalanine enantiomers
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l-Phenylalanine molecularly imprinted polymers were synthesized by bulk polymerization. Methacrylic acid and acrylamide were tested as functional monomers. Ethanol and acetonitrile were used as porogenic solvents. Optimal composition of polymerization mixture was methacrylic acid, template, and ethylene glycol dimethacrylate in molar ratio 1:5:26. MIP was applied as HPLC chiral stationary phase. The influences of the mobile phase composition, flow rate, column temperature, and column length on the efficiency of enantioseparation were investigated. The enantioselective separation of phenylalanine was attained in reversed phase mode at 45 °C with acetonitrile/water containing 1.5% acetic acid (90/10, v/v) as mobile phase (resolution value was 1.49, selectivity factor was 1.38). Applicability of polymeric stationary phase prepared for l-phenylalanine was tested for analysis of dietary supplement sample. The UV detection limits for both enantiomers were 1 mg cm−3 (S/N = 3). Good linearity was observed from 1 to 10 mg cm−3.
KeywordsAmino acid High performance liquid chromatography Chiral sorbent Thermodynamics
This work was financially supported by the Slovak Research and Development Agency under the contract no. APVV-15-0355 and by project Excellent teams of young researchers at STU.
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