A novel mycovirus isolated from the plant-pathogenic fungus Alternaria dianthicola

Abstract

Here, we describe the molecular characterization of a novel mycovirus isolated from a phytopathogenic fungus, Alternaria dianthicola, which we have named “Alternaria dianthicola dsRNA virus 1” (AdRV1). AdRV1 has a genome of 3,014 bp that contains two non-overlapping open reading frames (ORF1 and 2) coding for a hypothetical protein and an RNA-dependent RNA polymerase (RdRp), respectively. Based on the RdRp, AdRV1 is phylogenetically related to some unclassified dsRNA mycoviruses, including Alternaria longipes dsRNA virus 1, and shows a distant relationship to members of the family Partitiviridae. To the best of our knowledge, this is the first report of mycovirus infecting A. dianthicola.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2

References

  1. 1.

    Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384

    CAS  Article  Google Scholar 

  2. 2.

    Ghabrial SA, Castón JR, Jiang D, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479:356–368

    Article  Google Scholar 

  3. 3.

    Pearson MN, Beever RE, Boine B, Arthur K (2009) Mycoviruses of filamentous fungi and their relevance to plant pathology. Mol Plant Pathol 10:115–128

    CAS  Article  Google Scholar 

  4. 4.

    Yang D, Wu M, Zhang J (2018) Sclerotinia minor endornavirus 1, a novel pathogenicity debilitation-associated mycovirus with a wide spectrum of horizontal transmissibility. Viruses 10:589

    Article  Google Scholar 

  5. 5.

    Nuss DL (2005) Hypovirulence: mycoviruses at the fungal-plant interface. Nat Rev Microbiol 3:632–642

    CAS  Article  Google Scholar 

  6. 6.

    Yu X, Li B, Fu Y (2010) A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proc Natl Acad Sci USA 107:8387–8392

    CAS  Article  Google Scholar 

  7. 7.

    Heiniger U (1994) Biological control of chestnut blight in Europe. Ann Rev Phytopathol 32:581–599

    Article  Google Scholar 

  8. 8.

    Xie J, Jiang D (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68

    CAS  Article  Google Scholar 

  9. 9.

    Sato Y, Castón JR, Suzuki N (2018) The biological attributes, genome architecture and packaging of diverse multi-component fungal viruses. Curr Opin Virol 33:55–65

    CAS  Article  Google Scholar 

  10. 10.

    Martin RR, Zhou J, Tzanetakis IE (2011) Blueberry latent virus: an amalgam of the Partitiviridae and Totiviridae. Virus 155:175–180

    CAS  Article  Google Scholar 

  11. 11.

    Lin Y, Zhang H, Zhao C, Liu S, Guo L (2015) The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28. Arch Virol 160:577–580

    CAS  Article  Google Scholar 

  12. 12.

    Koloniuk I, Hrabáková L, Petrzik K (2015) Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana. Arch Virol 160:1585–1588

    CAS  Article  Google Scholar 

  13. 13.

    Kotta-Loizou I, Sipkova J, Coutts RH (2015) Identification and sequence determination of a novel double-stranded RNA mycovirus from the entomopathogenic fungus Beauveria bassiana. Arch Virol 160:873–875

    CAS  Article  Google Scholar 

  14. 14.

    Woudenberg JHC, Groenewald JZ, Binder M, Crous PW (2013) Alternaria redefined. Stud Mycol 75:171–212

    CAS  Article  Google Scholar 

  15. 15.

    Thomma BP (2003) Alternaria spp.: from general saprophyte to specific parasite. Mol Plant Pathol 4:225–236

    CAS  Article  Google Scholar 

  16. 16.

    Duan CF, Long YQ, Chen H, Yang GH, Gui M, Liu GH (2015) First report of Alternaria dianthicola causing flower blight on carnation in China. Bulletin OEPP/EPPO 45:195–198

    Article  Google Scholar 

  17. 17.

    Maiti CK, Sen S, Paul AK, Acharya K (2007) First Report of Alternaria dianthicola Causing Leaf Blight on Withania somnifera from India. Plant Dis 91:467

    CAS  Article  Google Scholar 

  18. 18.

    Shepherd HS (1988) Viruslike particles in tentoxin-producing strains of Alternaria alternata. J Virol 62:3888–3891

    CAS  Article  Google Scholar 

  19. 19.

    Hayashi N, Tsuge T, Kobayashi H, Nishimura S (1988) The presence of double stranded RNAs in Alternaria alternata Japanese pear pathotype and their participation in AK-toxin productivity. Annu Phytopathol Soc Jpn 54:250–252

    Article  Google Scholar 

  20. 20.

    Aoki N, Moriyama H, Kodama M, Arie T, Teraoka T, Fukuhara T (2009) A novel mycovirus associated with four double-stranded RNAs affects host fungal growth in Alternaria alternata. Virus Res 140:179–187

    CAS  Article  Google Scholar 

  21. 21.

    Okada R, Ichinose S, Takeshita K, Urayama SI, Fukuhara T, Komatsu K, Arie T, Ishihara A, Egusa M, Kodama M, Moriyama H (2018) Molecular characterization of a novel mycovirus in Alternaria alternata manifesting two-sided effects: Down-regulation of host growth and upregulation of host plant pathogenicity. Virology 519:23–32

    CAS  Article  Google Scholar 

  22. 22.

    Shamsi W, Sato Y, Jamal A, Shahi S, Kondo H, Suzuki N, Bhatti MF (2019) Molecular and biological characterization of a novel botybirnavirus identified from a Pakistani isolate of Alternaria alternata. Virus Res 263:119–128

    CAS  Article  Google Scholar 

  23. 23.

    Komatsu K, Katayama Y, Omatsu T, Mizutani T, Fukuhara T, Kodama M, Arie T, Teraoka T, Moriyama H (2016) Genome sequence of a novel victorivirus identified in the phytopathogenic fungus Alternaria arborescens. Arch Virol 161:1701–1704

    CAS  Article  Google Scholar 

  24. 24.

    Komatsu K, Katayama Y, Omatsu T, Mizutani T, Fukuhara T, Kodama M, Arie T, Teraoka T, Moriyama H (2016) Genome sequence of a novel mitovirus identified in the phytopathogenic fungus Alternaria arborescens. Arch Virol 161:2627–2631

    CAS  Article  Google Scholar 

  25. 25.

    Ma G, Zhang X, Hua H, Zhou T, Wu X (2020) Molecular and biological characterization of a novel strain of Alternaria alternata chrysovirus 1 identified from the pathogen Alternaria tenuissima causing watermelon leaf blight. Virus Res. https://doi.org/10.1016/j.virusres.2020.197904

    Article  PubMed  Google Scholar 

  26. 26.

    Shang HH, Zhong J, Zhang RJ, Chen CY, Gao BD, Zhu HJ (2015) Genome sequence of a novel endornavirus from the phytopathogenic fungus Alternaria brassicicola. Arch Virol 160:1827–1830

    CAS  Article  Google Scholar 

  27. 27.

    Zhong J, Shang HH, Zhu CX, Zhu JZ, Zhu HJ, Hu Y, Gao BD (2016) Characterization of a novel single-stranded RNA virus, closely related to fusariviruses, infecting the plant pathogenic fungus Alternaria brassicicola. Virus Res 217:1–7

    CAS  Article  Google Scholar 

  28. 28.

    Chen Y, Shang HH, Yang HQ, Gao BD, Zhong J (2017) A mitovirus isolated from the phytopathogenic fungus Alternaria brassicicola. Arch Virol 162:286–92874

    Google Scholar 

  29. 29.

    Xiang J, Fu M, Hong N, Zhai L, Xiao F, Wang G (2017) Characterization of a novel botybirnavirus isolated from a phytopathogenic Alternaria fungus. Arch Virol 162:3907–3911

    CAS  Article  Google Scholar 

  30. 30.

    Morris TJ, Dodds JA (1979) Isolation and analysis of double stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    CAS  Article  Google Scholar 

  31. 31.

    Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    CAS  Article  Google Scholar 

  32. 32.

    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    CAS  Article  Google Scholar 

Download references

Acknowledgements

This research was supported by the China Postdoctoral Science Foundation (Grant no. 2018M641562), the Natural Science Foundation of Hunan Province, China (Grant no. 2018JJ3229), and the Hunan Innovation and entrepreneurship training Foundation for Undergraduates (grant no. S201910537057).

Author information

Affiliations

Authors

Corresponding authors

Correspondence to Bi Da Gao or Jie Zhong.

Ethics declarations

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Handling Editor: Robert H.A. Coutts.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 14 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Hu, Z., Guo, J., Da Gao, B. et al. A novel mycovirus isolated from the plant-pathogenic fungus Alternaria dianthicola. Arch Virol (2020). https://doi.org/10.1007/s00705-020-04700-9

Download citation