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Biological and molecular characterization of a bacteriophage infecting Xanthomonas campestris pv. campestris, isolated from brassica fields

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Abstract

Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot of crucifers. Here, we report a virus that infects Xcc isolated from brassica fields in Brazil. Morphological, molecular and phylogenetic analysis indicated that the isolated virus is a new member of the genus Pbunavirus, family Myoviridae, and we propose the name “Xanthomonas virus XC 2” for this virus. The isolated virus has a narrow host range, infecting only Xcc isolates, and it did not infect unrelated bacteria. These results indicate that the isolated bacteriophage is highly specific for Xcc and may be a potential agent for biological control.

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Acknowledgments

We thank Karla Ribeiro and Gilmar Valente (Núcleo de Microscopia e Microanálise, CCB/UFV) for assistance with TEM analysis, and Wagner Gonzaga Gonçalves and José Eduardo Serrão (Dep de Biologia Geral, UFV) for assistance in sample preparation. We also thank Rosa de Lima Ramos Mariano (Dep. de Agronomia, UFRPE), Elineide Barbosa de Souza (Dep. de Biologia, UFRPE), Acelino Couto Alfenas and José Rogério de Oliveira (Dep. de Fitopatologia, UFV) for kindly providing the bacterial isolates used in this work, and Marisa Vieira Queiroz (Dep. de Microbiologia) for providing pulse field material and equipment. FPS was the recipient of a CNPq graduate fellowship, ASX and RRR were recipients of FAPEMIG graduate fellowships, and FPB was a recipient of an SIF graduate fellowship.

Funding

This study was funded by grant APQ-01926-14 (FAPEMIG) to PAZ.

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Authors and Affiliations

  1. Departament of Microbiology, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    Fernanda Pereira da Silva, André da Silva Xavier, Fernanda Prieto Bruckner, Rafael Reis de Rezende & Poliane Alfenas-Zerbini

  2. Núcleo de Análise de Biomoléculas, Centro de Ciências Biológicas, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    Pedro Marcus Pereira Vidigal

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  1. Fernanda Pereira da Silva
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  2. André da Silva Xavier
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  3. Fernanda Prieto Bruckner
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  4. Rafael Reis de Rezende
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  5. Pedro Marcus Pereira Vidigal
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  6. Poliane Alfenas-Zerbini
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Correspondence to Poliane Alfenas-Zerbini.

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da Silva, F.P., Xavier, A.d., Bruckner, F.P. et al. Biological and molecular characterization of a bacteriophage infecting Xanthomonas campestris pv. campestris, isolated from brassica fields. Arch Virol 164, 1857–1862 (2019). https://doi.org/10.1007/s00705-019-04263-4

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