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Phytophthora infestans RNA virus 2, a novel RNA virus from Phytophthora infestans, does not belong to any known virus group

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Abstract

Phytophthora infestans is the causal agent of potato and tomato late blight. In this study, we obtained the complete genome sequence of a novel RNA virus from this plant pathogen, tentatively named “Phytophthora infestans RNA virus 2” (PiRV-2). The PiRV-2 genome is 11,170 nt in length and lacks a polyA tail. It contains a single large open reading frame (ORF) with short 5’ and 3’ untranslated regions. The ORF is predicted to encode a polyprotein of 3710 aa (calculated molecular weight, 410.94 kDa). This virus lacks significant similarity to any other known viruses, even in the conserved RNA-dependent RNA polymerase region. Phylogenetic analysis demonstrated that it did not cluster with any known virus group. We conclude that PiRV-2 belongs to a new virus family yet to be described. This virus was found to be faithfully transmitted through asexual reproduction.

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Author information

Authors and Affiliations

  1. Crop production and Pest Control Research Unit, ARS, USDA and Botany and Plant Pathology Department, Purdue University, West Lafayette, IN, USA

    Guohong Cai

  2. School of Integrative Plant Science, Cornell University, Ithaca, NY, USA

    Kevin Myers & William E. Fry

  3. Department of Plant Biology and Pathology, Rutgers The State University of New Jersey, New Brunswick, NJ, USA

    Bradley I. Hillman

Authors
  1. Guohong Cai
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  2. Kevin Myers
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  3. William E. Fry
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  4. Bradley I. Hillman
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Corresponding authors

Correspondence to Guohong Cai or Bradley I. Hillman.

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Handling Editor: Robert H.A. Coutts.

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Cai, G., Myers, K., Fry, W.E. et al. Phytophthora infestans RNA virus 2, a novel RNA virus from Phytophthora infestans, does not belong to any known virus group. Arch Virol 164, 567–572 (2019). https://doi.org/10.1007/s00705-018-4050-0

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