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Bovine viral diarrhea virus non-structural protein NS4B induces autophagosomes in bovine kidney cells

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Abstract

Bovine viral diarrhea virus (BVDV) is an important pathogen in cattle that causes economic losses in livestock industries. Autophagy is an essential cell system for the maintenance of homeostasis and is induced by various triggers, including infection by viruses. BVDV infection leads to autophagy in order to enhance its replication in cells. In this study, we investigated the effect of BVDV non-structural proteins on the induction of autophagosomes. We found that NS4B alone could induce autophagosomes, suggesting a novel and important function of NS4B in BVDV replication.

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Acknowledgements

We thank Dr. Atsushi Miyawaki (Riken, Japan) for the Venus/pCS2 plasmid.

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Authors and Affiliations

  1. Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Yuto Suda, Shin Murakami & Taisuke Horimoto

  2. Division of Viral Disease and Epidemiology, National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan

    Yuto Suda

Authors
  1. Yuto Suda
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  2. Shin Murakami
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  3. Taisuke Horimoto
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Correspondence to Taisuke Horimoto.

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Handling Editor: Zhenhai Chen.

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Suda, Y., Murakami, S. & Horimoto, T. Bovine viral diarrhea virus non-structural protein NS4B induces autophagosomes in bovine kidney cells. Arch Virol 164, 255–260 (2019). https://doi.org/10.1007/s00705-018-4045-x

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  • DOI: https://doi.org/10.1007/s00705-018-4045-x

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