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Archives of Virology

, Volume 164, Issue 1, pp 51–61 | Cite as

Characterization of mule deerpox virus in Florida white-tailed deer fawns expands the known host and geographic range of this emerging pathogen

  • Katherine A. Sayler
  • Kuttichantran Subramaniam
  • Jessica M. Jacob
  • Julia C. Loeb
  • William F. Craft
  • Lisa L. Farina
  • Nicole I. Stacy
  • Nissin Moussatche
  • Laurie Cook
  • John A. Lednicky
  • Samantha M. Wisely
  • Thomas B. WaltzekEmail author
Original Article

Abstract

Infections caused by mule deerpox virus (MDPV) have been sporadically reported in North American cervids. White-tailed deer (Odocoileus virginianus) fawns from a farm located in South Central Florida presented with ulcerative and crusting lesions on the coronary band as well as the mucocutaneous tissues of the head. Evaluation of the crusted skin lesions was undertaken using microscopic pathology and molecular techniques. A crusted skin sample was processed for virus isolation in four mammalian cell lines. The resulting isolate was characterized by negative staining electron microscopy and deep sequencing. Histopathologic evaluation of the skin lesions from the fawns revealed a hyperplastic and proliferative epidermis with ballooning degeneration of epidermal and follicular keratinocytes with intracytoplasmic eosinophilic inclusions. Electron microscopy of cell culture supernatant demonstrated numerous large brick-shaped particles typical of most poxviruses. Polymerase chain reaction assays followed by Sanger sequencing revealed a poxvirus gene sequence nearly identical to that of previous strains of MDPV. The full genome was recovered by deep sequencing and genetic analyses supported the Florida white-tailed deer isolate (MDPV-F) as a strain of MDPV. Herein, we report the first genome sequence of MDPV from a farmed white-tailed deer fawn in the South Central Florida, expanding the number of locations and geographic range in which MDPV has been identified.

Notes

Compliance with ethical standards

Funding

This study was funded by the University of Florida, Institute of Food and Agricultural Science Cervidae Health Research Initiative, with funds provided by the State of Florida legislature.

Conflict of interest

All the authors declare that they have no conflict of interest.

Ethical approval

All animal procedures were reviewed and approved by the Institutional Animal Care and Use Committee at University of Florida (IACUC Protocol #: 201609390).

Supplementary material

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Supplementary material 1 (PDF 1105 kb)
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Supplementary material 2 (DOCX 11 kb)
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Supplementary material 3 (DOCX 30 kb)
705_2018_3991_MOESM4_ESM.docx (91 kb)
Supplementary material 4 (DOCX 83 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Katherine A. Sayler
    • 1
  • Kuttichantran Subramaniam
    • 2
  • Jessica M. Jacob
    • 2
  • Julia C. Loeb
    • 3
    • 4
  • William F. Craft
    • 5
  • Lisa L. Farina
    • 5
  • Nicole I. Stacy
    • 6
  • Nissin Moussatche
    • 7
  • Laurie Cook
    • 8
  • John A. Lednicky
    • 3
    • 4
  • Samantha M. Wisely
    • 1
  • Thomas B. Waltzek
    • 2
    Email author
  1. 1.Department of Wildlife Ecology and ConservationUniversity of FloridaGainesvilleUSA
  2. 2.Department of Infectious Diseases and Immunology, College of Veterinary MedicineUniversity of FloridaGainesvilleUSA
  3. 3.Department of Environmental and Global Health, College of Public Health and Health ProfessionsUniversity of FloridaGainesvilleUSA
  4. 4.Emerging Pathogens Institute, University of FloridaGainesvilleUSA
  5. 5.Department of Comparative, Diagnostic and Population Medicine, College of Veterinary MedicineUniversity of FloridaGainesvilleUSA
  6. 6.Department of Large Animal Clinical Sciences, College of Veterinary MedicineUniversity of FloridaGainesvilleUSA
  7. 7.Department of Molecular Genetics and MicrobiologyUniversity of FloridaGainesvilleUSA
  8. 8.BDRL Whitetail Paradise FarmOkeechobeeUSA

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