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Molecular detection and sequence analysis of porcine circovirus type 3 in sow sera from farms with prolonged histories of reproductive problems in Hunan, China

  • Yawen Zou
  • Nanxiangzi Zhang
  • Jiaxin Zhang
  • Sujiao Zhang
  • Yifan Jiang
  • Dongliang Wang
  • Qinghui Tan
  • Yi Yang
  • Naidong Wang
Brief Report

Abstract

A newly emerging porcine circovirus, designated PCV3, has been reported in various countries (USA, Poland, South Korea and China) since 2017. Its presence may be associated with porcine dermatitis and nephropathy syndrome (PDNS), reproductive failure, and multisystem inflammation. In this study, we report identification of PCV3 in cases of reproductive failure in various regions in Hunan, China. From January 2015 to December 2016, sera were collected from 190 sows from seven farms with reproductive problems. Specifically, 85 samples were from sows with a history of reproductive failure, whereas the remaining 105 were from healthy sows. The PCV3-positive rate was significantly higher in sows with reproductive failure (45.9%) than in healthy sows (21.9%), based on quantitative PCR (qPCR) assays. Although phylogenetic analysis based on the cap gene suggested that these PCV3 isolates belonged to the clade PCV3a, amino acid sequence variations in the Cap protein still occurred among these isolates, and these might have contributed to antigenic alterations of the Cap protein, based on the Jameson–Wolf antigenic index. Finally, we concluded that PCV3 was circulating in sows in Hunan province, China. However, the association of PCV3 with reproductive failure in sows and its potential for vertical transmission need to be studied further.

Notes

Acknowledgements

This project was supported by the Research Foundation of Hunan Provincial Education Department, China (Grant no. 15A086), Hunan Provincial Natural Science Foundation of China (Grant no. 2018JJ2177), and Postgraduate Research and Innovation Project of Hunan Province (Grant nos. CX2016B285 and CX2016B314).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest regarding publication of this paper.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_3914_MOESM1_ESM.docx (5.8 mb)
Supplementary material 1 (DOCX 5910 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Yawen Zou
    • 1
  • Nanxiangzi Zhang
    • 1
  • Jiaxin Zhang
    • 1
  • Sujiao Zhang
    • 1
  • Yifan Jiang
    • 1
  • Dongliang Wang
    • 1
  • Qinghui Tan
    • 1
  • Yi Yang
    • 1
  • Naidong Wang
    • 1
  1. 1.The Key Laboratory of Animal Vaccine and Protein Engineering, College of Veterinary MedicineHunan Agricultural UniversityChangshaChina

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