Archives of Virology

, Volume 163, Issue 10, pp 2849–2853 | Cite as

Genomic analysis of a novel isolate Heliothis virescens ascovirus 3i (HvAV-3i) and identification of ascoviral repeat ORFs (aros)

  • Zi-Shu Chen
  • Dian-Hai Hou
  • Xiao-Wen Cheng
  • Xing Wang
  • Guo-Hua Huang
Brief Report


Ascoviruses are circular double-stranded DNA viruses that infect insects. Herein we sequenced and analyzed the genome of the previously unrecorded ascovirus isolate Heliothis virescens ascovirus 3i (HvAV-3i). The genome size is 185,650 bp with 181 hypothetical open reading frames (ORFs). Additionally, definition based on ascovirus repeated ORFs (aros) is proposed; whereby the 29 aros from all sequenced Ascoviridae genomes are divided into six distinct groups. The topological relationship among the isolates of Heliothis virescens ascovirus 3a is (HvAV-3f, {HvAV-3h, [HvAV-3e, (HvAV-3g, HvAV-3i)]}) with every clade well supported by a Bayesian posterior probability of 1.00 and a Bootstrap value of 100%.



The authors would like to thank Jue Hu, Hai-Zhou Liu and Lei Zhang for their help in virus amplification and data analysis. The authors would also like to acknowledge Bi-Chao Xu from the Core Facility and Technical Support of Wuhan Institute of Virology for the technical assistance in SEM and TEM analysis. We thank Dr. Cecil L. Smith (University of Georgia, U.S.A.) for editing and revising the English language.


This study was partially supported by the National Natural Science Foundation of China (No. 31371995) and STS Project of the Chinese Academy of Sciences (No. KFJ-SW-STS143-3).

Compliance with ethical standards

Ethical approval and consent to participate

Not required.

Consent for publication

All authors consent to publication.

Availability of data and supporting materials

Supplemental material is available online.

Conflict of interest

The authors have declared that no competing interests exist. This article does not contain any studies with human or animal subjects performed by any of the authors.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Zi-Shu Chen
    • 1
    • 2
  • Dian-Hai Hou
    • 3
  • Xiao-Wen Cheng
    • 4
  • Xing Wang
    • 1
    • 2
  • Guo-Hua Huang
    • 1
    • 2
  1. 1.Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect PestsHunan Agricultural UniversityChangshaChina
  2. 2.Institute of Virology, College of Plant ProtectionHunan Agricultural UniversityChangshaChina
  3. 3.School of Bioscience and TechnologyWeifang Medical UniversityWeifangChina
  4. 4.Department of Microbiology, 56 Pearson HallMiami UniversityOxfordUSA

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