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Phylogenetic analysis of porcine reproductive and respiratory syndrome virus isolates from Northern Ireland

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Abstract

To investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in Northern Ireland, the ORF5 gene from nine field isolates was sequenced and phylogenetically analysed. The results revealed relatively high diversity amongst isolates, with 87.6-92.2% identity between farms at the nucleotide level and 84.1-93.5% identity at the protein level. Phylogenetic analysis confirmed that all nine isolates belonged to the European (type 1) genotype and formed a cluster within the subtype 1 subgroup. This study provides the first report on PRRSV isolate diversity in Northern Ireland.

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Acknowledgements

The authors would like to thank the Department of Agriculture, Environment and Rural Affairs (DAERA) for funding this study.

Funding

This study was part of a Ph.D. studentship funded by the Department of Agriculture, Environment and Rural Affairs (DAERA) for Northern Ireland.

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Authors and Affiliations

  1. Veterinary Science Division, Agri-Food and Biosciences Institute, Stormont, Belfast, BT4 3SD, UK

    Natalie Smith & John McKillen

  2. Centre for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Medical Biology Centre, Queen’s University Belfast, Belfast, BT9 7BL, UK

    Natalie Smith & Ultan F. Power

Authors
  1. Natalie Smith
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  2. Ultan F. Power
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Correspondence to John McKillen.

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All of the authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Zhenhai Chen.

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Smith, N., Power, U.F. & McKillen, J. Phylogenetic analysis of porcine reproductive and respiratory syndrome virus isolates from Northern Ireland. Arch Virol 163, 2799–2804 (2018). https://doi.org/10.1007/s00705-018-3886-7

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  • DOI: https://doi.org/10.1007/s00705-018-3886-7

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