Abstract
Three cycloviruses (genus Cyclovirus, family Circoviridae) were recovered from a dragonfly (Odonata: Anisoptera) captured in Fuzhou, China. The three cycloviruses, named dragonfly associated cyclovirus 9, 10 and 11 (DfCyV-9, -10, -11), respectively, show 56.1-79.6% genome-wide identity to known cycloviruses and 61.6-65.1% among themselves. Thus, according to the current species demarcation criteria, they represent three novel cycloviruses. Notably, DfCyV-10 has a predicted replication-associated protein (Rep) that is most similar to that of bat associated cyclovirus 2 (BatACyV-2), a cyclovirus discovered in China, with 79.4% amino acid sequence identity, but a putative capsid protein (Cp) most similar to that of BatACyV-10, a cyclovirus discovered in Brazil, with 71.7% amino acid sequence identity. These data are useful for understanding the diversity and evolution of cycloviruses, especially those found in insects.
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Acknowledgments
This work was supported by a fund from the State Tobacco Monopoly Administration (110201601024(LS-04)) and an FAFU fund for excellent young scholars (xjq201622). We are grateful to Dr. Fangluan Gao at the College of Plant Protection, Fujian Agriculture and Forestry University, for his help in constructing the phylogenetic tree.
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Supplementary Fig. 1 Pairwise comparisons among dragonfly associated cyclovirus 9, 10 and 11 (DfCyV-9, -10, and -11) and members of the genus Cyclovirus, including identity values based on the replication-associated (Rep) (A) and capsid (Cp) (B) protein coding sequences
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Islam, S.U., Lin, W., Wu, R. et al. Complete genome sequences of three novel cycloviruses identified in a dragonfly (Odonata: Anisoptera) from China. Arch Virol 163, 2569–2573 (2018). https://doi.org/10.1007/s00705-018-3876-9
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DOI: https://doi.org/10.1007/s00705-018-3876-9