Identification and molecular characterization of a novel varicosa-like virus from red clover
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During aetiological study of diseased red clover (Trifolium pratense L.) using high throughput sequencing, a novel virus with a 10 kb genome divided into two segments was discovered. The virus, tentatively named red clover associated varicosavirus (RCaVV), is phylogenetically related to classifiable members of the genus Varicosavirus (family Rhabdoviridae, order Mononegavirales). Analysis of mRNA levels from the individual RCaVV genes suggested possible differences in transcription regulation between rhabdoviruses with divided and undivided genomes.
We are kindly indebted to Alena Matyášová for excellent technical assistance and to Hana Jakešová (Red clover and grass breeding, Hladké Životice, Czech Republic) for kindly providing us with the red clover HZ2 plant and to two anonymous reviewers for valuable comments and critical remarks.
This study was funded by institutional support RVO60077344 and grant LD15035 of the Ministry of Education Youth and Sports of the Czech Republic and supported by the COST Action FA1407 (DIVAS), COST (European Cooperation in Science and Technology).
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Conflict of interest
All authors declare that they have no conflict of interest.
This article does not contain any studies with human participants performed by any of the authors.
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