Abstract
As part of an initiative to characterize viruses infecting Cape gooseberry in the province of Antioquia (Colombia), we report the genome sequence of a new member of the genus Ilarvirus (family Bromoviridae). This virus was identified in a Cape gooseberry plot in the municipality of Marinilla in a mixed infection with potato virus Y (PVY) as part of high-throughput sequencing initiative. Results were confirmed by nested RT-PCR and DAS-ELISA. Phylogenetic analysis suggested that the Cape gooseberry ilarvirus is a new member of subgroup 1 and it is most closely related to ageratum latent virus (AgLV). The name “Cape gooseberry ilarvirus 1” (CGIV-1) is proposed for this new ilarvirus.
Similar content being viewed by others
References
Fisher G, Almanza-Merchán PJ, Miranda D (2014) Importancia y cultivo de la uchuva (Physalis peruviana L.). Rev Bras Frutic 36:1–15
Agronet (2016) Red de información y comunicación del sector agropecuario Colombiano. http://www.agronet.gov.co/Paginas/default.aspx. Accessed 15 July 2017
Gutierrez P, Alzate JF, Montoya MM (2015) Complete genome sequence of an isolate of potato virus X (PVX) infecting Cape gooseberry (Physalis peruviana) in Colombia. Virus Genes 50:518–522
Muñoz-Baena L, Gutierrez P, Marin M (2016) Detección y secuenciación del genoma del potato virus Y (PVY) que infecta plantas de tomate en Antioquia, Colombia. Bioagro 28:69–80
Gutierrez P, Jaramillo-Mesa H, Marin M (2016) Genome sequence of a divergent Colombian isolate of potato virus V (PVV) infecting Solanum phureja. Acta virol 60:49–54
Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q, Chen Z, Mauceli E et al (2011) Full-length transcriptome assembly from RNA-seq data without a reference genome. Nat Biotechnol 29:644–652
Li H, Durbin R (2009) Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics 24:1754–1760
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for Bigger Datasets. Mol Biol Evol 33:1870–1874
Aboughanem-Sabanadzovic N, Tzanetakis IE, Lawrence A, Stephenson RC, Sabanadzovic S (2016) A novel ilarvirus is associated with privet necrotic ringspot disease in the Southern United States. Phytopathology 106:87–93
Maliogka VI, Dovas CI, Katis NI (2007) Demarcation of ilarviruses based on the phylogeny of RNA2-encoded RdRp and a generic ramped annealing RT-PCR. Arch Virol 152:1687–1698
O’Reilly EK, Kao CC (1998) Analysis of RNA-dependent RNA polymerase structure and function as guided by known polymerase structures and computer predictions of secondary structure. Virology 252:287–303
Pallas V, Aparicio V, Herranz MC, Sanchez-Navarro JA, Scott SW (2013) The molecular biology of ilarviruses. Adv Virus Res 87:139–181
King AMQ, Lefkowitz E, Adams MJ, Carstens EB (2011) Virus taxonomy. Elsevier, London
Funding
This work was funded by Universidad Nacional de Colombia through Grant VRI: 36705.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
All of the authors declare that we have no conflict of interest.
Ethical approval
This article does not contain any studies with human participants or animals performed by any of the authors.
Additional information
Handling Editor: F. Murilo Zerbini.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Gallo-García, Y.M., Jaramillo-Mesa, H., Toro-Fernández, L.F. et al. Characterization of the genome of a novel ilarvirus naturally infecting Cape gooseberry (Physalis peruviana). Arch Virol 163, 1713–1716 (2018). https://doi.org/10.1007/s00705-018-3796-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-018-3796-8