Establishment of a multiplex real-time RT-PCR assay for rapid identification of H6 subtype avian influenza viruses
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The H6 subtype avian influenza viruses (AIVs) possess the capacity for zoonotic transmission from avian species to humans. Establishment of a specific, rapid and sensitive method to screen H6 AIVs is necessary. Based on the conserved domain of the matrix and H6 AIV hemagglutinin genes, two TaqMan minor-groove-binder probes and multiplex real-time RT-PCR primers were designed in this study. The multiplex real-time RT-PCR assay developed in this study had high specificity and repeatability and a detection limit of 30 copies per reaction. This rapid diagnostic method will be useful for clinical detection and surveillance of H6 AIVs in China.
KeywordsAvian influenza virus H6 subtype Detection Rapid Sensitivity
This study was funded by grants from the National Science Foundation of the People’s Republic of China (81502852), Zhejiang Provincial Natural Science Foundation of China (LY15H190003), and the Independent Task of State Key Laboratory for Diagnosis and Treatment of Infectious Diseases (Nos. 2017ZZ10 and 2018ZZ06).
Compliance with ethical standards
Conflict of interest
The authors declare that they had no conflict of interest.
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