Viral shedding and clinical status of feline-norovirus-infected cats after reinfection with the same strain
Norovirus (NoV) infection is the most common cause of acute gastroenteritis in humans of all ages worldwide. When cats are experimentally infected with feline norovirus (FNoV), they develop symptoms of acute gastroenteritis. Therefore, FNoV infection may serve as an animal model for the disease caused by human norovirus infection. In this study, we examined whether FNoV of cats infected with genogroup GVI are protected from reinfection with the same strain. The blood anti-FNoV IgG level was inversely correlated with the viral load in stool samples and the clinical score of FNoV-infected cats, but complete prevention of reinfection was not observed. These findings were similar to the results of a reinfection experiment with NoV in human volunteers.
This study was in part funded by JSPS KAKENHI Grant number JP16K08027, and Kitasato University Research Grant for Young Researchers (2016).
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest.
All applicable national and institutional guidelines for the care and use of animals were followed. The animal experimentation protocol was approved by the President of Kitasato University through the judgment of the Institutional Animal Care and Use Committee of Kitasato University (Approval nos. 16-087 and 17-032). Specific-pathogen-free (SPF) cats were maintained in a temperature-controlled isolation facility. Sample sizes were determined based on our experience with FNoV infection models, and the minimum number of cats was used.
- 24.Takanashi S, Hashira S, Matsunaga T, Yoshida A, Shiota T, Tung PG, Khamrin P, Okitsu S, Mizuguchi M, Igarashi T, Ushijima H (2009) Detection, genetic characterization, and quantification of norovirus RNA from sera of children with gastroenteritis. J Clin Virol 44(2):161–163CrossRefPubMedGoogle Scholar