Two distinct begomoviruses associated with an alphasatellite coinfecting Emilia sonchifolia in Thailand
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Emilia sonchifolia is a traditionally used medicinal plant that is widespread in tropical and subtropical regions of the world. Yellow vein symptoms were observed in E. sonchifolia plants in fields in the county of Koh Samui, Surat Thani Province, Thailand, in August 2015. Two distinct begomoviruses, designated TH4872-6 and TH4872-9, and an associated alphasatellite were obtained from an E. sonchifolia leaf sample (TH4872). Sequence analysis showed that the full-length sequence of TH4872-6 was most closely related to that of ageratum yellow vein China virus (AYVCNV), with 85.7% identity, suggesting that it is a novel begomovirus, while the TH4872-9 sequence closely resembled cotton leaf curl Multan virus (CLCuMuV) with 99.1% identity. The alphasatellite sequence showed the highest nucleotide sequence identity (92.8%) to an isolate of tobacco curly shoot alphasatellite (TbCSA) originating from China. Recombination analysis revealed that the isolate TH4872-6 is a potential recombinant begomovirus, derived from ageratum yellow vein virus (AYVV) and tobacco leaf curl Thailand virus (TbLCTHV). This study represents the first report of begomoviruses identified in E. sonchifolia in Thailand.
This work was supported by the National Natural Science Foundation of China (31560501) and Applied Basic Research Foundation of Yunnan Province (2014FD067).
Compliance with ethical standards
I have read and have abided by the statement of ethical standards for manuscripts submitted to Archives of Virology.
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
The authors declare that they have no conflict of interest.
- 3.King AMQ, Adams MJ, Carstcns EB, Lefkowitz EJ (2012) Virus taxonomy: ninth report of the international committee on taxonomy of viruses. Elsevier/Academic Press, San Diego, pp 351–372Google Scholar
- 13.Lozano G, Trenado HP, Fiallo-Olivé E, Chirinos D, Geraud-Pouey F, Briddon RW, Navas-Castillo J (2016) Characterization of non-coding DNA satellites associated with sweepoviruses (genus Begomovirus, Geminiviridae)—definition of a distinct class of begomovirus-associated satellites. Front Microbiol 7:162CrossRefPubMedPubMedCentralGoogle Scholar
- 19.Yang CX, Cui GJ, Zhang J, Weng XF, Xie LH, Wu ZJ (2008) Molecular characterization of a distinct begomovirus species isolated from Emilia sonchifolia. J Plant Pathol 90:475–478Google Scholar
- 20.Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh tissue. Phytochem Bull 19:11–15Google Scholar
- 30.Brown JK, Zerbini FM, Navas-Castillo J, Moriones E, RamosSobrinho R, Silva JCF, Fiallo-OlivéE Briddon RW, HernándezZepeda C, Idris A, Malathi VG, Martin DP, Rivera-Bustamante R, Ueda S, Varsani A (2015) Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Arch Virol 160:1593–1619CrossRefPubMedGoogle Scholar