Inhibition of dengue virus infection by small interfering RNAs that target highly conserved sequences in the NS4B or NS5 coding regions
Dengue fever is one of the most common viral infections in the world. Although a vaccine against dengue virus (DENV) has been approved in several countries, this disease is still considered a public health priority worldwide. The ability of three small interfering RNAs (FG-siRNAs) targeting conserved sequences in the NS4B and NS5 regions of the DENV genome to inhibit DENV replication was tested in vitro in both Vero and C6/36 cells. The FG-siRNAs were effective against DENV-1, -3, and -4, but not DENV-2. A fourth siRNA specifically targeting the NS5 region of the DENV-2 genome (SG-siRNA) was designed and tested against two different DENV-2 strains, showing high levels of inhibition in both mammalian and insect cells.
KeywordssiRNA Dengue virus RNA interference Arbovirus Antiviral
We thank Dr. Juan Salas (ENMH-IPN) for kindly donating DENV-1, 2, 3 and 4 reference strains, Dr. Maria Isabel Salazar (ENCB-IPN) for providing the DENV-2 Yucatan 17438 strain, and Dr. Alfonso Méndez Tenorio (ENCB-IPN) for his support in the thermodynamic analyses.
This project was supported by Secretaría de Investigación y Posgrado del Instituto Politécnico Nacional (20160619). VPM has a fellowship from CONACyT, Mexico.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
This article does not contain any studies with animals performed by any of the authors.
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