Comparative genomic analysis of novel bacteriophages infecting Vibrio parahaemolyticus isolated from western and southern coastal areas of Korea
Vibrio parahaemolyticus, a foodborne pathogen, has become resistant to antibiotics. Therefore, alternative bio-control agents such bacteriophage are urgently needed for its control. Six novel bacteriophages specific to V. parahaemolyticus (vB_VpaP_KF1~2, vB_VpaS_KF3~6) were characterized at the molecular level in this study. Genomic similarity analysis revealed that these six bacteriophages could be divided into two groups with different genomic features, phylogenetic grouping, and morphologies. Two groups of bacteriophages had their own genes with different mechanisms for infection, assembly, and metabolism. Our results could be used as a future reference to study phage genomics or apply phages in future bio-control studies.
KeywordsVibrio parahaemolyticus bacteriophage comparative analysis genomic similarity phylogenetic tree
This study was supported by the Main Research Program (E0152203-03) of the Korea Food Research Institute, funded by the Ministry of Science and ICT. It was also supported by a grant (CRC-16-01-KRICT) from the National Research Council of Science & Technology (NST) fund by the Korea government (MSIP).
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 3.Carlson K (2005) Working with bacteriophages: common techniques and methodological approaches. In: Kutter E, Sula- Kvelidze A (eds) Bacteriophages: biology and application. CRC Press, Boca Raton, pp 437–494Google Scholar
- 4.CDC (2013) Antibiotic resistance threats in the United States, 2013. Current 114. pp 5–6. Doi: CS239559-Bhttps://www.cdc.gov/drugresistance/pdf/ar-threats-2013-508.pdf
- 5.Centers for Disease Control and Prevention (2013) Vibrio Illness (Vibriosis): Vibrio parahaemolyticus. http://www.cdc.gov/vibrio/vibriop.html. Accessed 14 May 2017
- 22.National Center for Biotechnology Information (NCBI) Genome database https://www.ncbi.nlm.nih.gov/genome. Accessed 4 Sep 2017
- 23.Peng F, Mi Z, Huang Y, Yuan X, Niu W, Wang Y, Hua Y, Fan H, Bai C, Tong Y (2014) Characterization, sequencing and comparative genomic analysis of vB_AbaM-IME-AB2, a novel lytic bacteriophage that infects multidrug-resistant Acinetobacter baumannii clinical isolates. BMC Microbiol 14:181CrossRefPubMedPubMedCentralGoogle Scholar
- 24.Stacy MC, Martha I, Jennifer YH, Patricia MG, Debra G, Alicia BC, Matthew C, Melissa TA, David B, Kirk S, Sarah L, Shelley Z, Paul RC, John D, Kristin GH, Susan L, Robert T, Olga LH (2014) Incidence and trends of infection with pathogens transmitted commonly through food-foodborne diseases active surveillance network, 10 U.S. sites, 2006-2013. MMWR 63(15):328–332Google Scholar
- 26.U.S Food and Drug Administration (FDA) Foodborne Illnesses: What You Need to Know. https://www.fda.gov/Food/FoodborneIllnessContaminants/FoodborneIllnessesNeedToKnow/default.htm. Accessed 1 Feb 2017