Molecular characterization of a divergent strain of calla lily chlorotic spot virus infecting celtuce (Lactuca sativa var. augustana) in China
Through sequencing and assembly of small RNAs, an orthotospovirus was identified from a celtuce plant (Lactuca sativa var. augustana) showing vein clearing and chlorotic spots in the Zhejiang province of China. The S, M, and L RNAs of this orthotospovirus were determined to be 3146, 4734, and 8934 nt, respectively, and shared 30.4-72.5%, 43.4-80.8%, and 29.84-82.9% nucleotide sequence identities with that of known orthotospoviruses. The full length nucleoprotein (N) of this orthotospovirus shared highest amino acid sequence identity (90.25%) with that of calla lily chlorotic spot virus isolated from calla lily (CCSV-calla) [China: Taiwan: 2001] and tobacco (CCSV-LJ1) [China: Lijiang: 2014]. Phylogenetic analyses showed that this orthotospovirus is phylogenetically associated with CCSV isolates and clustered with CCSV, tomato zonate spot virus (TZSV), and tomato necrotic spot-associated virus (TNSaV) in a separate sub-branch. These results suggest that this orthotospovirus is a divergent isolate of CCSV and was thus named CCSV-Cel [China: Zhejiang: 2017].
The authors would like to thank Dr. Zhongkai Zhang and Dr. Jiahong Dong from the Yunnan Academy of Agricultural Sciences for their valuable suggestions to this manuscript.
Compliance with ethical standards
Conflict of interest
All authors declare that no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 4.Lin YH, Chen TC, Hsu HT, Liu FL, Chu FH, Chen CC, Lin YZ, Yeh SD (2005) Serological comparison and molecular characterization for verification of Calla lily chlorotic spot virus as a new tospovirus species belonging to Watermelon silver mottle virus serogroup. Phytopathology 95:1482–1488CrossRefPubMedGoogle Scholar
- 6.Plyusnin A, Beaty BJ, Elliott RM, Goldbach R, Kormelink R, Lundkvist A, Schmaljohn CS, Tesh RB (2012) Family—Bunyaviridae. Virus Taxonomy. Elsevier, San Diego, pp 725–741Google Scholar