Archives of Virology

, Volume 163, Issue 5, pp 1375–1378 | Cite as

Molecular characterization of a divergent strain of calla lily chlorotic spot virus infecting celtuce (Lactuca sativa var. augustana) in China

Annotated Sequence Record

Abstract

Through sequencing and assembly of small RNAs, an orthotospovirus was identified from a celtuce plant (Lactuca sativa var. augustana) showing vein clearing and chlorotic spots in the Zhejiang province of China. The S, M, and L RNAs of this orthotospovirus were determined to be 3146, 4734, and 8934 nt, respectively, and shared 30.4-72.5%, 43.4-80.8%, and 29.84-82.9% nucleotide sequence identities with that of known orthotospoviruses. The full length nucleoprotein (N) of this orthotospovirus shared highest amino acid sequence identity (90.25%) with that of calla lily chlorotic spot virus isolated from calla lily (CCSV-calla) [China: Taiwan: 2001] and tobacco (CCSV-LJ1) [China: Lijiang: 2014]. Phylogenetic analyses showed that this orthotospovirus is phylogenetically associated with CCSV isolates and clustered with CCSV, tomato zonate spot virus (TZSV), and tomato necrotic spot-associated virus (TNSaV) in a separate sub-branch. These results suggest that this orthotospovirus is a divergent isolate of CCSV and was thus named CCSV-Cel [China: Zhejiang: 2017].

Notes

Acknowledgements

The authors would like to thank Dr. Zhongkai Zhang and Dr. Jiahong Dong from the Yunnan Academy of Agricultural Sciences for their valuable suggestions to this manuscript.

Compliance with ethical standards

Conflict of interest

All authors declare that no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_3743_MOESM1_ESM.docx (24 kb)
Supplementary material 1 (DOCX 23 kb)
705_2018_3743_MOESM2_ESM.pptx (62 kb)
Supplementary material 2 (PPTX 62 kb)

References

  1. 1.
    Adams MJ, Lefkowitz EJ, King AMQ et al (2017) Changes to taxonomy and the international code of virus classification and nomenclature ratified by the International Committee on Taxonomy of Viruses (2017). Arch Virol 162:2505–2538CrossRefPubMedGoogle Scholar
  2. 2.
    Amroun A, Priet S, de Lamballerie X, Quérat G (2017) Bunyaviridae RdRps: structure, motifs, and RNA synthesis machinery. Crit Rev Microbiol 43:753–778CrossRefPubMedGoogle Scholar
  3. 3.
    Chen CC, Chen TC, Lin YH, Yeh SD, Hsu HT (2005) A chlorotic spot disease on calla lilies (Zantedeschia spp.) is caused by a tospovirus serologically but distantly related to Watermelon silver mottle virus. Plant Dis 89:440–445CrossRefGoogle Scholar
  4. 4.
    Lin YH, Chen TC, Hsu HT, Liu FL, Chu FH, Chen CC, Lin YZ, Yeh SD (2005) Serological comparison and molecular characterization for verification of Calla lily chlorotic spot virus as a new tospovirus species belonging to Watermelon silver mottle virus serogroup. Phytopathology 95:1482–1488CrossRefPubMedGoogle Scholar
  5. 5.
    Liu Y, Lu X, Zhi L, Zheng Y, Chen X, Xu Y, Wu F, Li Y (2012) Calla lily chlorotic spot virus from spider lily (Hymenocallis litteralis) and tobacco (Nicotiana tabacum) in the Southwest of China. J Phytopathol 160:201–205CrossRefGoogle Scholar
  6. 6.
    Plyusnin A, Beaty BJ, Elliott RM, Goldbach R, Kormelink R, Lundkvist A, Schmaljohn CS, Tesh RB (2012) Family—Bunyaviridae. Virus Taxonomy. Elsevier, San Diego, pp 725–741Google Scholar
  7. 7.
    Scholthof K-BG, Adkins S, Czosnek H, Palukaitis P, Jacquot E, Hohn T, Hohn B, Saunders K, Candresse T, Ahlquist P, Hemenway C, Foster GD (2011) Top 10 plant viruses in molecular plant pathology. Mol Plant Pathol 12:938–954CrossRefPubMedGoogle Scholar
  8. 8.
    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Turina M, Kormelink R, Resende R (2016) Resistance to tospoviruses in vegetable crops: epidemiological and molecular aspects. Ann Rev Phytopathol 54:347–371CrossRefGoogle Scholar
  10. 10.
    Wang Y, Cheng X, Wu X, Wang A, Wu X (2014) Characterization of complete genome and small RNA profile of Pagoda yellow mosaic associated virus, a novel badnavirus in China. Virus Res 188:103–108CrossRefPubMedGoogle Scholar
  11. 11.
    Wu Q, Ding S-W, Zhang Y, Zhu S (2015) Identification of viruses and viroids by next-generation sequencing and homology-dependent and homology-independent algorithms. Ann Rev Phytopathol 53:425–444CrossRefGoogle Scholar
  12. 12.
    Xu Y, Wang SB, Li YZ, Tao HZ, Huang YN, Wu BW, Dong YM, Hu J, Liu YT (2016) Complete genome sequence of a distinct Calla lily chlorotic spot virus isolated in mainland China. Arch Virol 161:219–222CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of AgricultureNortheast Agricultural UniversityHarbinPeople’s Republic of China

Personalised recommendations