Archives of Virology

, Volume 163, Issue 5, pp 1387–1389 | Cite as

Molecular characterization and complete genome of a novel nepovirus from red clover

  • Igor Koloniuk
  • Jaroslava Přibylová
  • Jana Fránová
Annotated Sequence Record


During high throughput sequencing (HTS) of leaves from a symptomatic red clover plant, a new RNA virus, tentatively named red clover nepovirus A (RCNVA), was discovered. The complete genomic sequence was determined and characterized. Particularly noteworthy was that RCNVA shares high sequence identities in RNA1 with a group of phylogenetically related nepoviruses while homologies in the RNA2 segments are markedly lower. Based on the genomic organization and phylogenetic attributes, RCNVA should be classified as a novel virus of the genus Nepovirus (subfamily Comovirinae, family Secoviridae, order Picornavirales).



We are kindly indebted to Alena Matyášová for excellent technical assistance and anonymous reviewers for very constructive comments.


This study was funded by institutional support RVO60077344 and grant LD15035 of the Ministry of Education Youth and Sports of the Czech Republic and supported by the COST Action FA1407 (DIVAS), COST (European Cooperation in Science and Technology).

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

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Supplementary material 1 (FASTA 23 kb)
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Supplementary material 3 (PDF 1108 kb)
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Supplementary material 5 (PDF 63 kb)
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Supplementary material 6 (PDF 27 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Plant Virology, Biology Centre, Institute of Plant Molecular BiologyThe Czech Academy of SciencesČeské BudějoviceCzech Republic

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