Molecular characterization and complete genome of a novel nepovirus from red clover
Abstract
During high throughput sequencing (HTS) of leaves from a symptomatic red clover plant, a new RNA virus, tentatively named red clover nepovirus A (RCNVA), was discovered. The complete genomic sequence was determined and characterized. Particularly noteworthy was that RCNVA shares high sequence identities in RNA1 with a group of phylogenetically related nepoviruses while homologies in the RNA2 segments are markedly lower. Based on the genomic organization and phylogenetic attributes, RCNVA should be classified as a novel virus of the genus Nepovirus (subfamily Comovirinae, family Secoviridae, order Picornavirales).
Notes
Acknowledgements
We are kindly indebted to Alena Matyášová for excellent technical assistance and anonymous reviewers for very constructive comments.
Funding
This study was funded by institutional support RVO60077344 and grant LD15035 of the Ministry of Education Youth and Sports of the Czech Republic and supported by the COST Action FA1407 (DIVAS), COST (European Cooperation in Science and Technology).
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest.
Ethical approval
This article does not contain any studies with human participants performed by any of the authors.
Supplementary material
References
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