The first complete genome sequence of narcissus latent virus from Narcissus
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The complete sequence of a narcissus virus isolated from the Netherlands (Narv-NL) was determined to be 8172 nucleotides in length with an open reading frame encoding for 2624 amino acids. Phylogenetic analysis indicates that Narv-NL is clustered with high confidence among representative members from the genus Macluravirus, including artichoke latent virus (ArLV) and Chinese yam necrotic mosaic virus (CYNMV). Sequence analyses indicated Narv-NL shares 67%-69% nucleotide and 51%-68% amino acid sequence identity with ArLV and CYNMV either in the complete ORF or the coat protein (CP) gene, whereas it had 81%-99 % nucleotide and 80%-99 % amino acid sequence identity with the corresponding CP sequences of narcissus latent virus (NLV) isolates, suggesting that Narv-NL is a member of NLV. To our knowledge, this is the first report of the complete sequence of a NLV isolate.
We thank Dr Fengping Chen (Fujian Agriculture and Forestry University), Dr Sally Potter (The Australian National University) and two anonymous reviewers for comments and suggestions about the manuscript.
This work was supported by Fujian Natural Science Funds for Distinguished Young Scholar (No. 2014J06008); the National Natural Science Foundation of China (No. 31570146); the Public Science and Technology Research Funds Projects of General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China (No. 201410076); Scientific and Technological Program of Fuzhou (No. 2013-N-54) and the Program of Fujian Entry-Exit Inspection and Quarantine Bureau of China (No. FK2011-07).
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Conflict of interest
The authors declare that they have no conflicts of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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