Evidence for a complex of emergent poleroviruses affecting pepper worldwide
In recent years, symptoms of vein yellowing and leaf roll in pepper crops associated with the presence of poleroviruses (genus Polerovirus, family Luteoviridae) have been emerging in many countries worldwide. Spain was the first country in Europe where the yellowing disease of pepper was observed. In this work, a polerovirus isolate from Spain that infects pepper and has been shown to be transmitted by the aphid Aphis gossyppii (Spain-Almería 2-2013) was sequenced and compared with isolates from Japan, Israel, China and Australia. The genome (6125 nt in length, GenBank accession number KY523072) of the isolate from Spain has the typical organization of poleroviruses and contains seven open reading frames (ORF0 to ORF5 and ORF3a), putatively encoding proteins P0 to P5 and P3a. A comparison of the sequence from Spain with the four complete sequences available for poleroviruses infecting pepper showed a closer relationship to the isolate from Israel and supports the existence of a complex of at least five polerovirus species. Given that the symptoms caused by all pepper poleroviruses described to date are similar, if not identical, we propose to name them “pepper vein yellows virus 1” to “pepper vein yellows virus 5” (PeVYV-1 to PeVYV-5), with PeVYV-5 corresponding to the polerovirus from Spain described in this work. Our results and those published over the last few years have shown that the emergent poleroviruses threatening pepper crops around the world are highly complex due to recombination events.
We are thankful to Paloma Castillo (Laboratory of Plant Production and Health, Regional Government of Andalusia, Almería, Spain) for assistance with field sample collection after consent obtained from farmers, María Dolores Alcázar Alba with identification of aphids, and Francisco Villanueva and Remedios Tovar with aphid transmission and plant growing.
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Conflict of interest
The authors declare that they have no conflict of interest.
No studies involving human participants or animals performed by any of the authors are described in this article.
- 2.Gniffke PA, Shieh SC, Lin SW, Sheu ZM, Chen JR, Ho FI et al (2013) Pepper research and breeding at AVRDC—the world vegetable center. In: XV EUCARPIA meeting on genetics and breeding of capsicum and eggplant; Sep 2–4; Turin, Italy. pp 305–311Google Scholar
- 6.Antignus Y, Lachman O, Pearlsman M, Ucko O (2001) A new pepper yellowing disease of pepper caused by an unidentified luteovirus. Phytoparasitica 29:255Google Scholar
- 8.Domier LL (2012) Family Luteoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy. Ninth report of the International Committee on Taxonomy of Viruses. Elsevier/Academic Press, London, pp 1045–1053Google Scholar
- 9.International Committee on Taxonomy of Viruses (ICTV). Virus Taxonomy: 2016 Release. http://www.ictvonline.org/virustaxonomy.asp. Accessed 15 Nov 2017
- 10.Brault V, Herrbach E, Rodriguez-Medina C (2011) Luteoviruses. eLS. Wiley, ChichesterGoogle Scholar
- 11.Fereres A, Raccah B (2015) Insect transmission of plant viruses. eLS. Wiley, ChichesterGoogle Scholar
- 12.Smirnova E, Firth AE, Miller WA, Scheidecker D, Brault V, Reinbold C, Rakotondrafara AM, Chung BYW, Ziegler-Graff V (2015) Discovery of a small non-AUG-initiated ORF in poleroviruses and luteoviruses that is required for long-distance movement. PLoS Pathog 11:e1004868CrossRefPubMedPubMedCentralGoogle Scholar
- 14.Dombrovsky A, Glanz E, Lachman O, Sela N, Doron-Faigenboim A, Antignus Y (2013) The complete genomic sequence of Pepper yellow leaf curl virus (PYLCV) and its implications for our understanding of evolution dynamics in the genus Polerovirus. PLoS One 8:e70722CrossRefPubMedPubMedCentralGoogle Scholar
- 22.Bolou Bi BA, Moury B, Abo K, Kakou D Jr, Girardot G, Kouassi N’dri P et al (2015) First report of Pepper vein yellows virus in field grown pepper in Ivory Coast. J Plant Pathol 97:S67–S77Google Scholar
- 31.Lole KS, Bollinger RC, Paranjape RS, Gadkari D, Kulkarini SS, Novak NG, Ingersoll R, Sheppard HW, Ray SC (1999) Full-length Human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India with evidence of intersubtype recombination. J Virol 73:152–160PubMedPubMedCentralGoogle Scholar