Targeting of rice grassy stunt virus pc6 protein to plasmodesmata requires the ER-to-Golgi secretory pathway and an actin-myosin VIII motility system
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The nonstructural protein pc6 encoded by rice grassy stunt virus (RGSV) plays a significant role in viral cell-to-cell movement, presumably by transport through plasmodesmata (PD). We confirmed the association of pc6 with PD, and also elucidated the mechanisms of protein targeting to PD. Several inhibitor treatments showed conclusively that pc6 is targeted to PD via the ER-to-Golgi secretory system and actin filaments. In addition, VIII-1 myosin was also found to be involved in pc6 PD targeting. Deletion mutants demonstrated that C-terminal amino acid residues 209-229 (transmembrane domain 2; TM2) are essential for pc6 to move through PD.
KeywordsRice grassy stunt virus pc6 plasmodesmata movement protein protein targeting
This work was supported by the Natural Science Foundation of Fujian Province, China (Grant number 2014J06011) and the Science Fund for Distinguished Young Scholars of Fujian Agriculture and Forestry University (Grant number xjq201402).
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Conflict of interest
All authors declare that they have no conflict of interest.
This article does not contain any studies that used human participants.
- 3.Toriyama S, Tomaru K (1995) Genus tenuivirus. In: Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, Martelli GP, Mayo MA, Summers MD (eds) Virus taxonomy. Sixth report of the international committee on taxonomy of viruses. Springer, Vienna, pp 316–318Google Scholar
- 17.Wei T, Zhang C, Hong J, Xiong R, Kasschau KD, Zhou X, Carrington JC, Wang A (2010) Formation of complexes at plasmodesmata for Potyvirus intercellular movement is mediated by the viral protein P3N-PIPO. Plos Pathog 6:e1000962. https://doi.org/10.1371/journal.ppat.1000962 CrossRefPubMedPubMedCentralGoogle Scholar
- 22.Avisar D, Prokhnevsky AI, Makarova KS, Koonin EV, Dolja VV (2008) Myosin XI-K required for rapid trafficking of Golgi stacks, peroxisomes, and mitochondria in leaf cells of Nicotiana benthamiana. Am Soc Plant Biol 146:1098–1108Google Scholar
- 25.Ritzenthaler C, Nebenführ A, Movafeghi A, Stussi-Garaud C, Behnia L, Pimpl P, Staehelin LA, Robinson DG (2002) Reevaluation of the effects of Brefeldin A on plant cells using tobacco bright yellow 2 cells expressing Golgi-targeted green fluorescent protein and COPI antisera. Plant Cell 14:237–261CrossRefPubMedPubMedCentralGoogle Scholar