Archives of Virology

, Volume 163, Issue 5, pp 1317–1323 | Cite as

Targeting of rice grassy stunt virus pc6 protein to plasmodesmata requires the ER-to-Golgi secretory pathway and an actin-myosin VIII motility system

  • Xuelian Sui
  • Xiaojuan Liu
  • Wenwu Lin
  • Zujian WuEmail author
  • Liang YangEmail author
Brief Report


The nonstructural protein pc6 encoded by rice grassy stunt virus (RGSV) plays a significant role in viral cell-to-cell movement, presumably by transport through plasmodesmata (PD). We confirmed the association of pc6 with PD, and also elucidated the mechanisms of protein targeting to PD. Several inhibitor treatments showed conclusively that pc6 is targeted to PD via the ER-to-Golgi secretory system and actin filaments. In addition, VIII-1 myosin was also found to be involved in pc6 PD targeting. Deletion mutants demonstrated that C-terminal amino acid residues 209-229 (transmembrane domain 2; TM2) are essential for pc6 to move through PD.


Rice grassy stunt virus pc6 plasmodesmata movement protein protein targeting 



This work was supported by the Natural Science Foundation of Fujian Province, China (Grant number 2014J06011) and the Science Fund for Distinguished Young Scholars of Fujian Agriculture and Forestry University (Grant number xjq201402).

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies that used human participants.


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Province Key Laboratory of Plant Virology, Institute of Plant VirologyFujian Agriculture and Forestry UniversityFuzhouChina

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