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Full genome sequence of the first bluetongue virus serotype 21 (BTV-21) isolated from China: evidence for genetic reassortment between BTV-21 and bluetongue virus serotype 16 (BTV-16)

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Abstract

Bluetongue (BT) is one of the most important insect-borne, non-contagious viral diseases of ruminants and can cause severe disease and death in sheep. Its pathogen, bluetongue virus (BTV) has a double-stranded RNA genome consisting of 10 segments that provides an opportunity for field and vaccine strains of different serotypes to reassort whilst simultaneously infecting the same animal. For the first time, we report the full-length genome sequence of a BTV strain of serotype 21 (5149E) isolated from sentinel cattle in Guangxi Province in China in 2015. Sequence analysis suggested that the isolate 5149E had undergone a reassortment incident and acquired seg-6 from an isolate of BTV-16 which originated from Japan. This study aims to provide more understanding as to the origin and epidemiology of BTV.

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Acknowledgements

We are grateful to Associate Prof. Shaoqiang Ruan at the College of Foreign Language, Guangxi University, for her help in preparing the manuscript.

Funding

This study was funded by the Special Fund for Agro-scientific Research in the Public Interest of China (Project No. 201303035), the Open Project Foundation of State Key Laboratory of Veterinary Biotechnology (Project No. SKLVBF201613), and the Special Fund for Basic Scientific Research of Guangxi (Project No. Guike 16-4).

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Author notes

  1. Shaomin Qin, Heng Yang and Yixuan Zhang contributed equally to this article.

Authors and Affiliations

  1. Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, Guangxi, China

    Shaomin Qin, Yixuan Zhang, Jun Lin, Yingying Cao, Pengfei Ren & Jianmin Wu

  2. Yunnan Tropical and Subtropical Animal Virus Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Jindian, Kunming, 650224, Yunnan, China

    Heng Yang, Zhanhong Li, Lin Gao, Defang Liao & Huachun Li

Authors
  1. Shaomin Qin
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  2. Heng Yang
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  3. Yixuan Zhang
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  4. Zhanhong Li
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  5. Jun Lin
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  7. Defang Liao
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  8. Yingying Cao
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  9. Pengfei Ren
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  10. Huachun Li
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  11. Jianmin Wu
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Corresponding authors

Correspondence to Huachun Li or Jianmin Wu.

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Conflict of interest

The authors declare no conflict of interests.

Ethical approval

The animal experiments were approved and performed in accordance with the animal ethics guidelines and approved procedures of Guangxi Veterinary Research Institute, and Yunnan Animal Science and Veterinary Institute.

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Handling Editor: Diego G. Diel.

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Qin, S., Yang, H., Zhang, Y. et al. Full genome sequence of the first bluetongue virus serotype 21 (BTV-21) isolated from China: evidence for genetic reassortment between BTV-21 and bluetongue virus serotype 16 (BTV-16). Arch Virol 163, 1379–1382 (2018). https://doi.org/10.1007/s00705-018-3718-9

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  • DOI: https://doi.org/10.1007/s00705-018-3718-9

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