Genetic analysis of rotavirus G2P strains in Pune, Western India: circulation of a novel reassortant bearing E6 NSP4 genotype
In India, G2P strains are known to be the second most predominant group A rotaviruses causing acute gastroenteritis among children. This study was performed to determine the diversity within VP7(G), VP4(P), VP6(I) and NSP4(E) genes of 16 G2P rotavirus strains detected in children hospitalized for acute gastroenteritis in Pune, Western India during 2009-2013. Fourteen strains showed G2-P-I2-E2 and two strains showed G2-P-I2-E6 genotype constellation. Phylogenetic analysis showed their clustering into G2-IV-a3, P-5bi/ii, I2-3ii and E2-4i/ii or E6 genotypes/lineages. These data reveal inter- and/or intra-genotypic variations in a genogroup-2 constellation of G2P rotavirus strains circulating in Pune, Western India, providing evidence of a novel G2P reassortant bearing a rare NSP4 genotype, E6 during 2009-2013.
The authors thank the Council of Scientific and Industrial Research (CSIR), New Delhi, India for supporting the first author (G. P.) with a Research Fellowship, and Dr D. T. Mourya, Director and Dr A. C. Mishra, Former Director, National Institute of Virology, Pune for their constant support.:
Compliance with ethical standards
This study was funded by the Indian Council of Medical Research (ICMR).
Conflicts of interest
The authors declare that there was no conflict of interest.
The study was approved by the Institutional Ethics Committee (Ref no: NIV/IHEC/2013/D-1874) of National Institute of Virology, Pune, India. Informed consent was obtained from parents/guardian of all children included in the study prior to sample collection.
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