Abstract
Root rot fungi of the genus Heterobasidion are highly destructive conifer pathogens in the northern Boreal forest region. This report describes the complete genome sequence of Heterobasidion partitivirus 20 infecting a Finnish strain of Heterobasidion annosum. The bisegmented dsRNA genome of HetPV20-an1 encodes a predicted RNA-dependent RNA polymerase of 605 amino acids (aa) and a capsid protein of 536 aa. Based on sequence similarity and phylogenetic analysis, this virus is a new member of the genus Alphapartitivirus. HetPV20-an1 shares ~65% RdRP aa sequence identity with the most similar virus strain, Rosellinia necatrix partitivirus 2, whereas the CP of HetPV20-an1 is most similar to that of rose partitivirus with ~27% overall aa sequence identity. HetPV20-an1 is only distantly related to previously known partitiviruses of Heterobasidion species and shares ~29% RdRP aa sequence identity and ~16% CP aa sequence identity with Heterobasidion partitivirus 1 from H. abietinum.
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Acknowledgements
Marja-Leena Santanen, Ari Rajala and Juha Puranen are acknowledged for technical assistance, and Tuula Piri is thanked for providing H. annosum strain S37-6.
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This study was funded by the Academy of Finland (grant number 309896).
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Eeva J. Vainio declares that she has no conflict of interest.
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This article does not contain any studies with human participants or animals performed by any of the authors.
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Handling Editor: Robert H.A. Coutts.
Nucleotide sequence data reported are available in the GenBank database under the accession numbers MG566085-6.
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Vainio, E.J. The complete genome sequence of HetPV20-an1, an alphapartitivirus infecting the conifer-pathogenic fungus Heterobasidion annosum. Arch Virol 163, 1113–1116 (2018). https://doi.org/10.1007/s00705-018-3707-z
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DOI: https://doi.org/10.1007/s00705-018-3707-z