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Archives of Virology

, Volume 163, Issue 4, pp 1107–1111 | Cite as

Complete genome sequence and construction of an infectious full-length cDNA clone of a German isolate of celery mosaic virus

  • Hanna Rose
  • Edgar Maiss
Annotated Sequence Record

Abstract

The complete genome sequence of a German isolate of celery mosaic virus (CeMV, a potyvirus) from Quedlinburg (DSMZ PV-1003) was determined (MF962880). This represents the second fully sequenced genome of this virus, along with a Californian isolate (HQ676607.1). The positive-sense single-stranded RNA is 10,000 nucleotides in length and shows the typical organization of potyviruses but has a shorter PIPO than CeMV California. In comparison to CeMV isolates from different origins, CeMV-Quedlinburg and isolates from the Netherlands (AF203531.1) and Aschersleben, Germany (AJ271087.1) show a NAG instead of DAG in the region of the coat protein responsible for aphid transmission. In this study the first infectious full-length clone of celery mosaic virus was obtained and the infectivity confirmed by Rhizobium radiobacter infiltration of Apium species.

Notes

Acknowledgements

The authors thank Heinrich Josef Vetten for critical reading the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_3705_MOESM1_ESM.pdf (125 kb)
Supplementary material 1 (PDF 125 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Horticultural Production Systems, Section PhytomedicineLeibniz University HannoverHannoverGermany

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