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Complete genome sequence and construction of an infectious full-length cDNA clone of a German isolate of celery mosaic virus

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Abstract

The complete genome sequence of a German isolate of celery mosaic virus (CeMV, a potyvirus) from Quedlinburg (DSMZ PV-1003) was determined (MF962880). This represents the second fully sequenced genome of this virus, along with a Californian isolate (HQ676607.1). The positive-sense single-stranded RNA is 10,000 nucleotides in length and shows the typical organization of potyviruses but has a shorter PIPO than CeMV California. In comparison to CeMV isolates from different origins, CeMV-Quedlinburg and isolates from the Netherlands (AF203531.1) and Aschersleben, Germany (AJ271087.1) show a NAG instead of DAG in the region of the coat protein responsible for aphid transmission. In this study the first infectious full-length clone of celery mosaic virus was obtained and the infectivity confirmed by Rhizobium radiobacter infiltration of Apium species.

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References

  1. Severin HHP, Freitag JH (1935) California celery-mosaic diseases. Phytopathology 25:891

    Google Scholar 

  2. Xu D, Liu H-Y, Li F et al (2011) Complete genome sequence of Celery mosaic virus and its relationship to other members of the genus Potyvirus. Arch Virol 156(5):917–920

    Article  CAS  PubMed  Google Scholar 

  3. Severin HHP, Freitag JH (1938) Western celery mosaic. Hilgardia 11(9):493–558

    Article  Google Scholar 

  4. Pemberton AW, Frost RR (1974) Celery mosaic virus in England. Plant Pathol 23(1):20–24

    Article  Google Scholar 

  5. Latham LJ, Jones RAC (2003) Incidence of Celery mosaic virus in celery crops in south-west Australia and its management using a ‘celery-free period’. Austral Plant Pathol 32(4):527

    Article  Google Scholar 

  6. Bos L, Mandersloot HJ, Vader F et al (1989) An epidemic of celery mosaic potyvirus in celeriac (Apium graveolens var. rapaceum) in the Netherlands. Netherl J Plant Pathol 95(4):225–240

    Article  Google Scholar 

  7. Chen J, Adams MJ (2001) A universal PCR primer to detect members of the Potyviridae and its use to examine the taxonomic status of several members of the family. Arch Virol 146(4):757–766

    Article  CAS  PubMed  Google Scholar 

  8. Fernández T, Carballo O, Zambrano K et al (2006) First report of celery mosaic virus infecting celery in Venezuela. Plant Dis 90(8):1111

    Article  PubMed  Google Scholar 

  9. Paduch-Cichal E, Sala-Rejczak K (2010) Celery mosaic virus occurring in Poland. Phytopathologia 57:45–48

    Google Scholar 

  10. Khoshkhatti N, Habibi-Koohi M, Mosahebi G et al (2011) Characterization of celery mosaic virus from celery in Tehran Province. Iran J of Virol 5(1):10–14

    Article  Google Scholar 

  11. Amal AA, Salwa NZ, Khatab Ema AH (2012) Characterization of celery mosaic virus isolated from some Apiaceae plants. Int J Virol 8(2):214–223

    Article  CAS  Google Scholar 

  12. Brandes J, Luisoni E (1966) Untersuchungen über einige Eigenschaften von zwei gestreckten Sellerieviren. J Phytopathol 57(3):277–288

    Article  Google Scholar 

  13. Moran J, van Rijswijk B, Traicevski V et al (2002) Potyviruses, novel and known, in cultivated and wild species of the family Apiaceae in Australia. Arch Virol 147(10):1855–1867

    Article  CAS  PubMed  Google Scholar 

  14. Menzel W, Jelkmann W, Maiss E (2002) Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control. J Virol Methods 99(1–2):81–92

    Article  CAS  PubMed  Google Scholar 

  15. Gibson DG, Young L, Chuang R-Y et al (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6(5):343–345

    Article  CAS  PubMed  Google Scholar 

  16. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166(4):557–580

    Article  CAS  PubMed  Google Scholar 

  17. Grimsley N, Hohn B, Hohn T et al (1986) “Agroinfection,” an alternative route for viral infection of plants by using the Ti plasmid. Proc Natl Acad Sci USA 83(10):3282–3286

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Hellens R, Mullineaux P, Klee H (2000) Technical focus: a guide to Agrobacterium binary Ti vectors. Trends Plant Sci 5(10):446–451

    Article  CAS  PubMed  Google Scholar 

  19. Tamura K, Stecher G, Peterson D et al (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12):2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32(5):1792–1797

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783–791

    Article  PubMed  Google Scholar 

  22. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425

    CAS  PubMed  Google Scholar 

  23. Hall TA (ed) (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT, vol 41. Information Retrieval Ltd., London, p 1979

    Google Scholar 

  24. Atreya CD, Raccah B, Pirone TP (1990) A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus. Virology 178(1):161–165

    Article  CAS  PubMed  Google Scholar 

  25. Atreya CD, Pirone TP (1993) Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility. Proc Natl Acad Sci USA 90(24):11919–11923

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The authors thank Heinrich Josef Vetten for critical reading the manuscript.

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Correspondence to Edgar Maiss.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Ralf Georg Dietzgen.

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Rose, H., Maiss, E. Complete genome sequence and construction of an infectious full-length cDNA clone of a German isolate of celery mosaic virus. Arch Virol 163, 1107–1111 (2018). https://doi.org/10.1007/s00705-018-3705-1

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