Archives of Virology

, Volume 163, Issue 5, pp 1325–1330 | Cite as

Human bocavirus in hospitalized children under 5 years with acute respiratory infection, São Paulo, Brazil, 2010

  • Patricia Evelin Silva
  • Cristina Adelaide Figueiredo
  • Adriana Luchs
  • Terezinha Maria de Paiva
  • Margarete Aparecida Benega Pinho
  • Renato Sousa Paulino
  • Daniela Bernardes Borges da Silva
  • Katia Corrêa de Oliveira Santos
  • Ana Maria Sardinha Afonso
  • Maria Isabel de Oliveira
Brief Report


The aims of this study were to investigate the human bocavirus (HBoV) frequency and genotypes in hospitalized children <5 years presenting acute respiratory infections (ARI) within the São Paulo metropolitan area. Nasopharyngeal samples from 300 patients, previously screened for common respiratory viruses, were tested by qPCR for the NSP1 and NP-1 genes. The VP1/2 gene in positive samples was then amplified by PCR and sequenced. A total of 49 positive HBoV cases (16.3%; mean Ct value of 34.41) were detected with the mean age being 18.1 months (range 1 month to 5 years) and the median age being 1 year of age. Children aged between 0 and 12 months had higher detection rates of HBoV (69.4%; 34/49; mean Ct = 34.45) than children from other age groups (30.6%; 15/49; mean Ct = 34.34). No significant differences were observed between HBoV Ct levels and clinical illness. The occurrence was more frequently associated with fall (38.8%; 19/49) and spring (36.7%; 18/49). All 12 sequenced isolates were identified as HBoV-1, displaying minor genetic variation compared to the Swedish reference strains ST1 and ST2 (99.1–99.7% nt). The sole identification of HBoV-1 supports the hypothesis that this particular genotype is strongly related to ARI, and contributes to the role of this virus in the aetiology of respiratory diseases.


Human bocavirus Real-time PCR Respiratory tract infection Surveillance Molecular epidemiology 


Compliance with ethical standards


Graduate Program in Science, Coordinator for Disease Control, Ministry of Health-PPG-CCD-SES/SP (partial support).

Conflict of interest

The authors declare that there is no conflict of interest.

Ethical approval

Previous ethics committee approval was granted by Adolfo Lutz Institute (CEPIAL) (no. 048/2011). This was an anonymous unlinked study and informed consent was not required according to the resolution 466/12 concerning research evolving humans beings -Conselho Nacional de Saúde (CNS)/Ministério da Saúde (MS), Brasília, 2012.


  1. 1.
    Arthur JL, Higgins GD, Davidson GP, Givney RC, Ratcliff RM (2009) A novel bocavirus associated with acute gastroenteritis in Australian children. PLoS Pathog 5(4):e1000391CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Kapoor A, Simmonds P, Slikas E, Li L, Bodhidatta L, Sethabutr O et al (2010) Human bocaviruses are highly diverse, dispersed, recombination prone, and prevalent in enteric infections. J Infect Dis 201(11):1633–1643CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Jartti T, Hedman K, Jartti L, Ruuskanen O, Allander T, Söderlund-Venermo M (2012) Human bocavirus-the first 5 years. Rev Med Virol 22(1):46–64CrossRefPubMedGoogle Scholar
  4. 4.
    Albuquerque MC, Rocha LN, Benati FJ, Soares CC, Maranhão AG, Ramírez ML et al (2007) Human bocavirus infection in children with gastroenteritis, Brazil. Emerg Infect Dis 13(11):1756–1758CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Sousa TT, Almeida TNV, Fiaccadori FS, Souza M, Badr KR, Cardoso DDDP (2017) Identification of Human Bocavirus type 4 in a child asymptomatic for respiratory tract infection and acute gastroenteritis - Goiânia, Goiás, Brazil. Braz J Infect Dis 21(4):472–476CrossRefPubMedGoogle Scholar
  6. 6.
    Bastien N, Chui N, Robinson JL, Lee BE, Dust K, Hart L et al (2007) Detection of human bocavirus in Canadian children in a 1-year study. J Clin Microbiol 45(2):610–613CrossRefPubMedGoogle Scholar
  7. 7.
    Guido M, Quattrocchi M, Campa A, Zizza A, Grima P, Romano A et al (2011) Human metapneumovirus and human bocavirus associated with respiratory infection in Apulian population. Virology 417(1):64–70CrossRefPubMedGoogle Scholar
  8. 8.
    Tran DN, Nguyen TQ, Nguyen TA, Hayakawa S, Mizuguchi M, Ushijima H (2014) Human bocavirus in children with acute respiratory infections in Vietnam. J Med Virol 86(6):988–994CrossRefPubMedGoogle Scholar
  9. 9.
    Lu QB, Wo Y, Wang HY, Huang DD, Zhao J, Zhang XA et al (2015) Epidemic and molecular evolution of human bocavirus in hospitalized children with acute respiratory tract infection. Eur J Clin Microbiol Infect Dis 34(1):75–81CrossRefPubMedGoogle Scholar
  10. 10.
    Gagliardi TB, Iwamoto MA, Paula FE, Proença-Modena JL, Saranzo AM, Criado MF et al (2009) Human bocavirus respiratory infections in children. Epidemiol Infect 137(7):1032–1036CrossRefPubMedGoogle Scholar
  11. 11.
    Souza EL, Ramos JG, Proença-Módena JL, Diniz A, Carvalho G, Ciuffo I et al (2010) Human bocavirus in very young infants hospitalized with acute respiratory infection in northeast Brazil. J Trop Pediatr 56(2):125–127CrossRefPubMedGoogle Scholar
  12. 12.
    Pilger DA, Cantarelli VV, Amantea SL, Leistner-Segal S (2011) Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil. Mem Inst Oswaldo Cruz 106(1):56–60CrossRefPubMedGoogle Scholar
  13. 13.
    Nascimento-Carvalho CM, Cardoso MR, Meriluoto M, Kemppainen K, Kantola K, Ruuskanen O et al (2012) Human bocavirus infection diagnosed serologically among children admitted to hospital with community-acquired pneumonia in a tropical region. J Med Virol 84(2):253–258CrossRefPubMedGoogle Scholar
  14. 14.
    Caccia ER, Watanabe AS, Carraro E, Leal E, Granato C, Bellei N (2012) Frequency of human bocavirus respiratory infections among at-risk patients in São Paulo, Brazil. Rev Inst Med Trop Sao Paulo 54(6):307–310CrossRefPubMedGoogle Scholar
  15. 15.
    do Amaral de Leon (2013) C, Amantea SL, Pilger DA, Cantarelli V. Clinical and epidemiologic profile of lower respiratory tract infections associated with human bocavirus. Pediatr Pulmonol 48(11):1112–1118CrossRefPubMedGoogle Scholar
  16. 16.
    Lu X, Chittaganpitch M, Olsen SJ, Mackay IM, Sloots TP, Fry AM et al (2006) Real-time PCR assays for detection of bocavirus in human specimens. J Clin Microbiol 44(9):3231–3235CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Emery SL, Erdman DD, Bowen MD, Newton BR, Winchell JM, Meyer RF et al (2004) Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus. Emerg Infect Dis 10(2):311–316CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Phillips G, Lopman B, Tam CC, Iturriza-Gomara M, Brown D, Gray J (2009) Diagnosing norovirus-associated infectious intestinal disease using viral load. BMC Infect Dis 9:63CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Sloots TP, McErlean P, Speicher DJ, Arden KE, Nissen MD, Mackay IM (2006) Evidence of human coronavirus HKU1 and human bocavirus in Australian children. J Clin Virol 35(1):99–102CrossRefPubMedGoogle Scholar
  20. 20.
    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12):2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Wong-Chew RM, García-León ML, Noyola DE, Pérez-González LF, Gaitán-Meza J, Villaseñor-Sierra A, et al (2017) Respiratory viruses detected in Mexican children younger than 5 years old with community-acquired pneumonia: a national multicenter study. Int J Infect Dis 62:32–38.
  22. 22.
    Deluca GD, Urquijo MC, Passarella C, Picón C, Picón D, Acosta M et al (2016) Bocavirus in infants under 5 years with acute respiratory infection. Chaco Province, Argentina, 2014. Medicina (B Aires) 76(3):135–138Google Scholar
  23. 23.
    Bharaj P, Sullender WM, Kabra SK, Broor S (2010) Human bocavirus infection in children with acute respiratory tract infection in India. J Med Virol 82(5):812–816CrossRefPubMedGoogle Scholar
  24. 24.
    Lee EJ, Kim HS, Kim HS, Kim JS, Song W, Kim M et al (2016) Human bocavirus in Korean children with gastroenteritis and respiratory tract infections. Biomed Res Int 2016:7507895PubMedPubMedCentralGoogle Scholar
  25. 25.
    Allander T, Jartti T, Gupta S, Niesters HG, Lehtinen P, Osterback R et al (2007) Human bocavirus and acute wheezing in children. Clin Infect Dis 44:904–910CrossRefPubMedGoogle Scholar
  26. 26.
    Pozo F, Garcia-Garcia ML, Calvo C, Cuesta I, Perez-Brena P, Casas I (2007) High incidence of human bocavirus infection in children in Spain. J Clin Virol. 40:224–228CrossRefPubMedGoogle Scholar
  27. 27.
    Choi EH, Lee HJ, Kim SJ, Eun BW, Kim NH, Lee JA et al (2006) The association of newly identified respiratory viruses with lower respiratory tract infections in Korean children, 2000–2005. Clin Infect Dis 43(5):585–592CrossRefPubMedGoogle Scholar
  28. 28.
    Ma X, Endo R, Ishiguro N, Ebihara T, Ishiko H, Ariga T et al (2006) Detection of human bocavirus in Japanese children with lower respiratory tract infections. J Clin Microbiol 44(3):1132–1134CrossRefPubMedPubMedCentralGoogle Scholar
  29. 29.
    Weissbrich B, Neske F, Schubert J, Tollmann F, Blath K, Blessing K et al (2006) Frequent detection of bocavirus DNA in German children with respiratory tract infections. BMC Infect Dis 6:109CrossRefPubMedPubMedCentralGoogle Scholar
  30. 30.
    Moesker FM, van Kampen JJ, van der Eijk AA, van Rossum AM, de Hoog M, Schutten M, Smits SL, Bodewes R, Osterhaus AD, Fraaij PL (2015) Human bocavirus infection as a cause of severe acute respiratory tract infection in children. Clin Microbiol Infect. 21(10):964.e1-8Google Scholar
  31. 31.
    Uršič T, Jevšnik M, Zigon N, Krivec U, Beden AB, Praprotnik M, Petrovec M (2012) Human bocavirus and other respiratory viral infections in a 2-year cohort of hospitalized children. J Med Virol. 84(1):99–108CrossRefPubMedGoogle Scholar
  32. 32.
    Zhao B, Yu X, Wang C, Teng Z, Wang C, Shen J, Gao Y, Zhu Z, Wang J, Yuan Z, Wu F, Zhang X, Ghildyal R (2013) High human bocavirus viral load is associated with disease severity in children under five years of age. PLoS One 8(4):e62318CrossRefPubMedPubMedCentralGoogle Scholar
  33. 33.
    Moreno B, Abrego L, Carrera JP, Franco D, Gaitán M, Castillo J et al (2016) Detection of human bocavirus type 1 infection in Panamanian children with respiratory illness. J Med Virol 88(3):389–394CrossRefPubMedGoogle Scholar
  34. 34.
    Martin ET, Kuypers J, McRoberts JP, Englund JA, Zerr DM (2015) Human bocavirus 1 primary infection and shedding in infants. J Infect Dis 212(4):516–524CrossRefPubMedPubMedCentralGoogle Scholar
  35. 35.
    Wang Y, Gonzalez R, Zhou H, Li J, Li Y, Paranhos-Baccalà G et al (2011) Detection of human bocavirus 3 in China. Eur J Clin Microbiol Infect Dis 30(6):799–805CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Patricia Evelin Silva
    • 1
  • Cristina Adelaide Figueiredo
    • 1
  • Adriana Luchs
    • 2
  • Terezinha Maria de Paiva
    • 1
  • Margarete Aparecida Benega Pinho
    • 1
  • Renato Sousa Paulino
    • 1
  • Daniela Bernardes Borges da Silva
    • 1
  • Katia Corrêa de Oliveira Santos
    • 1
  • Ana Maria Sardinha Afonso
    • 1
  • Maria Isabel de Oliveira
    • 1
  1. 1.Instituto Adolfo Lutz, Centro de Virologia, Núcleo de Doenças RespiratóriasSão PauloBrazil
  2. 2.Instituto Adolfo Lutz, Centro de Virologia, Núcleo de Doenças EntéricasSão PauloBrazil

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