Archives of Virology

, Volume 163, Issue 3, pp 799–803 | Cite as

Genome characterization of an Argentinean isolate of alfalfa leaf curl virus

  • Nicolás Bejerman
  • Verónica Trucco
  • Soledad de Breuil
  • Patricia Rodriguez Pardina
  • Sergio Lenardon
  • Fabián Giolitti
Annotated Sequence Record


We investigated the molecular characteristics of an Argentinean isolate of alfalfa leaf curl virus (ALCV-Arg), a virus of the genus Capulavirus in the family Geminiviridae that was isolated from alfalfa plants showing dwarfism. The genome was found to be 2,750 nucleotides in length. In pairwise comparisons, this ALCV isolate shared 83.2% to 92.6% sequence identity with European ALCV isolates. Sequence comparisons and phylogenetic analysis showed that this isolate combines features of strains A and B of ALCV. Recombination analysis showed that ALCV-Arg is a recombinant isolate that was generated by intraspecific recombination between ALCV strains A and B. The results of this study not only show that ALCV-Arg is unique because it combines features of strains A and B but also show that ALCV naturally infects this forage crop on the American continent.



This work was partially supported by the PNPV 1135022 project of INTA.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


This work was partially supported by the PNPV 1135022 project of INTA.

Supplementary material

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Supplementary material 1 (DOCX 18 kb)
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Supplementary material 4 (DOCX 13 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  • Nicolás Bejerman
    • 1
    • 2
  • Verónica Trucco
    • 1
  • Soledad de Breuil
    • 1
    • 2
  • Patricia Rodriguez Pardina
    • 1
  • Sergio Lenardon
    • 1
    • 3
  • Fabián Giolitti
    • 1
  1. 1.IPAVE-CIAP-INTACórdobaArgentina
  2. 2.Concejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290 (1425 FBQ) CABABuenos AiresArgentina
  3. 3.Facultad de Agronomía y Veterinaria (FAV)Universidad Nacional de Río Cuarto (UNRC)Río CuartoArgentina

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