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Identification of a new begomovirus infecting Duranta repens in Pakistan

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Abstract

Ornamental perennial plants may serve as reservoirs for viruses that infect field crops. Duranta repens is an ornamental shrub that frequently exhibits leaf curl symptoms typical of begomoviral infection. Two begomoviruses or DNA-A components (isolates 57SA and 58SA) along with a DNA-B component (31SA) were identified in a symptomatic D. repens plant. Isolates 57SA and 58SA showed the highest nucleotide sequence identity of 90% and 92.2% to catharanthus yellow mosaic virus (CaYMV) and chilli leaf curl India virus (ChiLCINV), respectively, whereas 31SA shared the highest nucleotide sequence identity of 95.2% with the DNA-B component of tomato leaf curl New Delhi Virus (ToLCNDV). Isolate 57SA (showing < 91% highest nucleotide sequence identity) may thus be considered a member of a distinct species, and we propose the name “duranta leaf curl virus” (DLCV) for this virus. In addition, isolate 58SA (showing highest nucleotide sequence identity < 94%) is a newly identified strain of ChiLCINV for which the name “Duranta” strain is proposed. Further analysis showed that 57SA, 58SA and 31SA are recombinant, again highlighting the importance of recombination in begomovirus evolution. This is the first identification of DLCV. Also, a mixed infection with two viruses (or DNA-A components) and one DNA-B is reported.

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Acknowledgements

Anonymous reviewers for their critical comments on the manuscript are highly acknowledged.

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Authors and Affiliations

  1. Plant Biotechnology Department, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Sector H-12, Islamabad, Pakistan

    Sidra Anwar & Muhammad Tahir

Authors
  1. Sidra Anwar
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  2. Muhammad Tahir
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Correspondence to Muhammad Tahir.

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The authors declare no conflict of interest.

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by any of the authors.

Funding

The study was funded by Higher Education Commission (HEC), Pakistan, under research grant number 1689.

Additional information

Handling Editor: Jesus Navas-Castillo.

Electronic supplementary material

Below is the link to the electronic supplementary material.

705_2017_3672_MOESM1_ESM.jpg

Supplementary Figure 1 Photographs of (A) a non-symptomatic and (B) infected Duranta repens plants from which the viruses identified here were isolated (JPEG 1327 kb)

705_2017_3672_MOESM2_ESM.jpg

Supplementary Figure 2 Multiple sequence alignment of the intergenic region of 31SA with that of papaya leaf crumple virus (PaLCrV; DNA-A; KJ028210), AM392426 (ToLCNDV DNA-B-like) and selected ToLCNDV DNA-B sequences from the nucleotide sequence databases. The iteron sequences (GGTGT) of ToLCNDV DNA-B and AM392426 are highlighted in yellow. However, iterons GGTGT in 31SA might be replaced by GGTGA and GGTAC sequence present in PaLCrV. (JPEG 24 kb)

Supplementary material 3 (DOCX 17 kb)

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Anwar, S., Tahir, M. Identification of a new begomovirus infecting Duranta repens in Pakistan. Arch Virol 163, 809–813 (2018). https://doi.org/10.1007/s00705-017-3672-y

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  • DOI: https://doi.org/10.1007/s00705-017-3672-y

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