Species A rotaviruses isolated from hospitalized patients over 5 years of age in Kolkata, India, in 2012/13
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In hospital-based diarrhoeal disease surveillance at Infectious Diseases & Beliaghata-General Hospital (May-2012 to April-2013), Kolkata, India, stool samples were collected from patients < 5 years (n = 830) and > 5 years of age (n = 728) hospitalized with diarrhea. Group-A rotavirus (GARV) was identified by ELISA followed by multiplex RT-PCR. In children < 5 years of age, 53.4% of the samples were positive for GARV. In patients > 5 years to 90 years old, only 6.04% (n = 44) tested positive for GARV. G2P strains (n = 16 [36.36%]) were the most prevalent, followed by G9P strains (n = 13 [29.54%]), while P-(n = 30 [68.18%]) was most prevalent among the P genotypes. The GARV strains G2, G9 and P detected in adults clustered together in the phylogenetic tree with the GARV strains identified in children (< 5 years) during the same period. Rotavirus positivity was high among female patients (75%), suggesting that caregivers (mother/grandmother/older-siblings) may get infected through young children or may act as carriers for transmission.
Keywordsrotavirus adults diarrhea Kolkata India
Indian Council of Medical Research (ICMR), New Delhi, India, and National Institute of Infectious Diseases (NIID), Tokyo, Japan
Compliance with ethical standards
This study was carried out accordance with the Institutional Ethics Committee guidelines letter no. C-48/2012-T & E.
Conflict of interest
The author declares that there are no conflicts of interest.
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