Isolation and genomic characterization of a novel avian orthoreovirus strain in Korea, 2014
In this study, we isolated a novel avian reovirus (ARV) strain, K738/14, from a broiler chicken with viral arthritis in South Korea. Genome sequence comparisons showed relatively low nucleotide identity with previously identified ARV strains. Phylogenetic analyses suggested multiple reassortment events between reovirus strain S1133 and reoviruses of Hungarian, Chinese, and US origin had occurred. In addition, recombination analyses showed evidence of intra-segmental recombination in the M2 and S2 genes. Based on our genetic analyses, multiple reassortment events, intra-segmental recombination, and accumulation of point mutations have possibly contributed to the emergence of this novel genotype of ARV, identified in Korea.
KeywordsAvian reovirus Genetic analysis Reassortment Intra-segmental recombination Point mutation
We thank Woo-tack Hong, Gyeong-bin Gwon, Kyu-jik Kim for excellent technical assistance.
Compliance with ethical standards
This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Animal Disease Management Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Grant number: 115099-1).
Conflict of interest
The authors have no conflicts of interest to declare.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 1.Ayalew LE, Gupta A, Fricke J, Ahmed KA, Popowich S, Lockerbie B, Tikoo SK, Ojkic D, Gomis S (2017) Phenotypic, genotypic and antigenic characterization of emerging avian reoviruses isolated from clinical cases of arthritis in broilers in Saskatchewan, Canada. Sci Rep 7:3565CrossRefPubMedPubMedCentralGoogle Scholar
- 6.David ES (2013) Disease of poultry, 13th edn. Wiley, New YorkGoogle Scholar