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Archives of Virology

, Volume 163, Issue 3, pp 633–637 | Cite as

Detection of hepatitis B virus DNA and HBsAg from postmortem blood and bloodstains

  • Junpei Hara
  • Yuka Tanaka
  • Hiroto Kaneko
  • Yoshito Itoh
  • Hiroshi Ikegaya
Original Article

Abstract

A large number of accidental virus infections occur in medical and non-medical workers exposed to infectious individuals and materials. We evaluated whether postmortem blood and bloodstains containing hepatitis B virus (HBV) are infectious. HBV-infected blood and bloodstains were stored for up to 60 days at room temperature and subsequently screened for hepatitis B surface antigen (HBsAg) and HBV DNA. In addition, HBV-positive postmortem blood was added to a cell line and the production of HBV virions was examined over a period of 7 days. HBsAg and HBV DNA were detected in all samples stored for 60 days at room temperature. HBV-positive postmortem blood successfully infected the cell line and progeny viruses were produced for up to 6 days. Thus, it is crucial that due care is taken when handling not only living material infected with HBV, as well as other harmful viruses, but also blood or body fluids from cadavers or medical waste.

Notes

Acknowledgements

We are grateful to the blood sample donors. Language editing services were provided by ThinkSCIENCE, Tokyo, Japan.

Compliance with ethical standards

Funding

This work was supported by a Grant-in-Aid for Scientific Research C from the Japan Society for the Promotion of Science (no. 22590641).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This study was approved by the institutional review board of Kyoto Prefectural University of Medicine (G-52).

Informed consent

Informed consent was obtained from all individual participants included in the study.

References

  1. 1.
    Victory KR, Coronado F, Ifono SO, Soropogui T, Dahl BA, Centers for Disease Control and Prevention (CDC) (2015) Ebola transmission linked to a single traditional funeral ceremony—Kissidougou, Guinea, December 2014-January 2015. MMWR Morb Mortal Wkly Rep 64:386–388PubMedGoogle Scholar
  2. 2.
    Yanagi M (2016) The group infection of tuberculosis. Doctors in The University of Tokyo were also infected. The Mainichi (In Japanese)Google Scholar
  3. 3.
    Takasaka T, Itoh Y, Kaneko H, Ikegaya H (2011) Detection of hepatitis C virus and antibodies in postmortem blood and bloodstains. J Clin Microbiol 49:1122–1123CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Ramirez S, Mikkelsen LS, Gottwein JM, Bukh J (2016) Robust HCV genotype 3a infectious cell culture system permits identification of escape variants with resistance to sofosbuvir. Gastroenterology 151:973–985CrossRefPubMedGoogle Scholar
  5. 5.
    Ott JJ, Stevens GA, Groeger J, Wiersma ST (2012) Global epidemiology of hepatitis B virus infection: new estimates of age-specific HBsAg seroprevalence and endemicity. Vaccine 30:2212–2219CrossRefPubMedGoogle Scholar
  6. 6.
    Lavanchy D (2005) Worldwide epidemiology of HBV infection, disease burden, and vaccine prevention. J Clin Virol 34(Suppl 1):S1–S3CrossRefPubMedGoogle Scholar
  7. 7.
    Coppola N, De Pascalis S, Onorato L, Calò F, Sagnelli C, Sagnelli E (2016) Hepatitis B virus and hepatitis C virus infection in healthcare workers. World J Hepatol 8:273–281CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Sells MA, Chen ML, Acs G (1987) Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA. Proc Natl Acad Sci USA 84:1005–1009CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Ladner SK, Otto MJ, Barker CS, Zaifert K, Wang GH, Guo JT, Seeger C, King RW (1997) Inducible expression of human hepatitis B virus (HBV) in stably transfected hepatoblastoma cells: a novel system for screening potential inhibitors of HBV replication. Antimicrob Agents Chemother 41:1715–1720PubMedPubMedCentralGoogle Scholar
  10. 10.
    Mizokami M, Nakano T, Orito E, Tanaka Y, Sakugawa H, Mukaide M, Robertson BH (1999) Hepatitis B virus genotype assignment using restriction fragment length polymorphism patterns. FEBS Lett 450:66–71CrossRefPubMedGoogle Scholar
  11. 11.
    Nannini P, Sokal EM (2016) Hepatitis B: changing epidemiology and interventions. Arch Dis Child.  https://doi.org/10.1136/archdischild-2016-312043 [Epub ahead of print] PubMedGoogle Scholar
  12. 12.
    Thomas DL, Factor SH, Kelen GD, Washington AS, Taylor E Jr, Quinn TC (1993) Viral hepatitis in health care personnel at The Johns Hopkins Hospital. The seroprevalence of and risk factors for hepatitis B virus and hepatitis C virus infection. Arch Intern Med 153:1705–1712CrossRefPubMedGoogle Scholar
  13. 13.
    Murokawa H, Yoshikawa A, Ohnuma H, Iwata A, Katoh N, Miyamoto M, Mine H, Emura H, Tadokoro K, Japanese Red Cross NAT Screening Research Group (2005) Epidemiology of blood donors in Japan, positive for hepatitis B virus and hepatitis C virus by nucleic acid amplification testing. Vox Sang 88:10–16CrossRefPubMedGoogle Scholar
  14. 14.
    Zanetti AR, Mariano A, Romanò L, D’Amelio R, Chironna M, Coppola RC, Cuccia M, Mangione R, Marrone F, Negrone FS, Parlato A, Zamparo E, Zotti C, Stroffolini T, Mele A, Study Group (2005) Long-term immunogenicity of hepatitis B vaccination and policy for booster: an Italian multicentre study. Lancet 366:1379–1384CrossRefPubMedGoogle Scholar
  15. 15.
    US Public Health Service (2001) Updated US public health service guidelines for the management of occupational exposures to HBV, HCV, and HIV and recommendations for postexposure prophylaxis. MMWR Recomm 50 (RR-11), pp 1–52Google Scholar
  16. 16.
    Zanetti AR, Van Damme P, Shouval D (2008) The global impact of vaccination against hepatitis B: a historical overview. Vaccine 26:6266–6273CrossRefPubMedGoogle Scholar
  17. 17.
    Mele A, Tosti ME, Mariano A, Pizzuti R, Ferro A, Borrini B, Zotti C, Lopalco P, Curtale F, Balocchini E, Spada E, National Surveillance System for Acute Viral Hepatitis (SEIEVA) Collaborating Group (2008) Acute hepatitis B 14 years after the implementation of universal vaccination in Italy: areas of improvement and emerging challenges. Clin Infect Dis 46:868–875CrossRefPubMedGoogle Scholar
  18. 18.
    Yan H, Zhong G, Xu G, He W, Jing Z, Gao Z, Huang Y, Qi Y, Peng B, Wang H, Fu L, Song M, Chen P, Gao W, Ren B, Sun Y, Cai T, Feng X, Sui J, Li W (2012) Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus. Elife 1:e00049CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Kullak-Ublick GA, Ismair MG, Kubitz R, Schmitt M, Häussinger D, Stieger B, Hagenbuch B, Meier PJ, Beuers U, Paumgartner G (2000) Stable expression and functional characterization of a Na+-taurocholate cotransporting green fluorescent protein in human hepatoblastoma HepG2 cells. Cytotechnology 34:1–9CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Neurath AR, Kent SB, Strick N, Parker K (1986) Identification and chemical synthesis of a host cell receptor binding site on hepatitis B virus. Cell 46:429–436CrossRefPubMedGoogle Scholar
  21. 21.
    Petit MA, Dubanchet S, Capel F, Voet P, Dauguet C, Hauser P (1991) HepG2 cell binding activities of different hepatitis B virus isolates: inhibitory effect of anti-HBs and anti-preS1 (21–47). Virology 180:483–491CrossRefPubMedGoogle Scholar
  22. 22.
    Qiao M, Macnaughton TB, Gowans EJ (1994) Adsorption and penetration of hepatitis B virus in a nonpermissive cell line. Virology 201:356–363CrossRefPubMedGoogle Scholar
  23. 23.
    Glebe D, Urban S (2007) Viral and cellular determinants involved in hepadnaviral entry. World J Gastroenterol 13:22–38CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Forensic Medicine, Graduate School of Medical ScienceKyoto Prefectural University of MedicineKyotoJapan
  2. 2.Molecular Gastroenterology and Hepatology, Graduate School of Medical ScienceKyoto Prefectural University of MedicineKyotoJapan
  3. 3.Aiseikai Yamashina HospitalKyotoJapan

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