Characterization of tomato leaf curl purple vein virus, a new monopartite New World begomovirus infecting tomato in Northeast Brazil
A new begomovirus species was identified from tomato plants with upward leaf curling and purple vein symptoms, which was first identified in the Piaui state of Northeast (NE) Brazil in 2014. Tomato leaf samples were collected in 2014 and 2016, and PCR with degenerate primers revealed begomovirus infection. Rolling circle amplification and restriction enzyme digestion indicated a single genomic DNA of ~ 2.6 kb. Cloning and sequencing revealed a genome organization similar to DNA-A components of New World (NW) bipartite begomoviruses, with no DNA-B. The complete nucleotide sequence had the highest identity (80%) with the DNA-A of Macroptilium yellow spot virus (MacYSV), and phylogenetic analyses showed it is a NW begomovirus that clusters with MacYSV and Blainvillea yellow spot virus, also from NE Brazil. Tomato plants agroinoculated with a dimeric clone of this genomic DNA developed upward leaf curling and purple vein symptoms, indistinguishable from those observed in the field. Based on agroinoculation, this virus has a narrow host range, mainly within the family Solanaceae. Co-inoculation experiments with tomato severe rugose virus and tomato mottle leaf curl virus, the two predominant begomoviruses infecting tomato in Brazil, revealed a synergistic interaction among these begomoviruses. The name Tomato leaf curl purple vein virus (ToLCPVV) is proposed for this new begomovirus.
We thank Mr. Leonardo da Fonte and Dr. Hasan Bolkan for kindly taking us to tomato fields in Piaui. A. K. Inoue-Nagata is a CNPq fellow.
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Conflict of interest
Authors declare that they have no conflict of interest.
This article did not contain any studies with human participants or animals performed by any of the authors.
- 2.Brown JK, Zerbini FM, Navas-Castillo J, Morinoes E, Ramos-Sorbinho R, Silva JC, Fiallo-Olivé E, Briddon RW, Hernández-Zepeda C, Idris A, Malathi VG, Martin DP, Rivera-Bustamante R, Ueda S, Varsani A (2015) Revision of begomovirus taxonomy based on pairwise sequence comparisons. Arch Virol 160:1593–1619CrossRefPubMedGoogle Scholar
- 3.Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
- 15.Lima AT, Sobrinho RR, González-Aguilera J, Rocha CS, Silva SJ, Xavier CA, Silva FN, Duffy S, Zerbini FM (2013) Synonymous site variation due to recombination explains higher genetic variability in begomovirus populations infecting non-cultivated hosts. J Gen Virol 94:418–431CrossRefPubMedGoogle Scholar
- 17.Melgarejo TA, Kon T, Rojas MR, Paz-Carrasco L, Zerbini FM, Gilbertson RL (2013) Characterization of a new world monopartite begomovirus causing leaf curl disease of tomato in Ecuador and Peru reveals a new direction in geminivirus evolution. J Virol 87:5397–5413CrossRefPubMedPubMedCentralGoogle Scholar
- 18.Morales FJ (2010) Distribution and dissemination of begomoviruses in Latin America and the Caribbean. In: Stansly PA, Naranjo SE (eds) Bemisia: bionomics management of a global Pest. Springer, Dordrecht, pp 283–318Google Scholar
- 21.Rambaut ADA (2010) Tree annotator version 1.6.1 (computer program). http://beast.bio.ed.ac.uk. Accessed 1 Aug 2010
- 26.Salati R, Nahkla MK, Rojas MR, Guzman P, Jaquez J, Maxwell DP, Gilbertson RL (2002) Tomato yellow leaf curl virus in the Dominican Republic: characterization of an infectious clone, virus monitoring in whiteflies, and identification of reservoir hosts. Phytopathology 92:487–496CrossRefPubMedGoogle Scholar
- 31.Vu S, Melgarejo TA, Chen L, Souza JO, Macedo MA, Inoue-Nagata AK, Gilbertson RL (2015) Evidence that tomato mottle leaf curl virus from Northeastern Brazil is an indigenous New World monopartite begomovirus. Phytopathology 105:S4.143Google Scholar