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Archives of Virology

, Volume 163, Issue 3, pp 719–724 | Cite as

Identification and characterization of a new potyvirus infecting cucurbits

  • M. C. Perotto
  • E. A. Pozzi
  • M. G. Celli
  • C. E. Luciani
  • M. S. Mitidieri
  • V. C. Conci
Brief Report

Abstract

A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.

Keywords

Potyvirus Squash Viruses Illumina sequencing 

Notes

Acknowledgements

This study was carried out at IPAVE-CIAP-INTA and was partially supported by INTA and CONICET. Dr. Luis Conci kindly provided positive samples.

Compliance with ethical standards

Funding

This study was funded by CONICET (PIP no. 112-20110111016) and INTA (PNHFA 1106072, PNPV 1135024).

Conflict of interest

All authors declare no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Instituto de Patología Vegetal (IPAVE-CIAP-INTA)CórdobaArgentina
  2. 2.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)CórdobaArgentina
  3. 3.Estación Experimental Agropecuaria San Pedro (EEA San Pedro-INTA)San PedroArgentina

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