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Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells

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Abstract

Autophagy is a homeostatic process that has been shown to be vital in the innate immune defense against pathogens. However, little is known about the regulatory role of autophagy in porcine teschovirus 2 (PTV-2) replication. In this study, we found that PTV-2 infection induces a strong increase in GFP-LC3 punctae and endogenous LC3 lipidation. However, PTV-2 infection did not enhance autophagic protein degradation. When cellular autophagy was pharmacologically inhibited by wortmannin or 3-methyladenine, PTV-2 replication increased. The increase in virus yield via autophagy inhibition was further confirmed by silencing atg5, which is required for autophagy. Furthermore, PTV-2 replication was suppressed when autophagy was activated by rapamycin. Together, the results suggest that PTV-2 infection activates incomplete autophagy and that autophagy then inhibits further PTV-2 replication.

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Acknowledgements

This work is partly supported by the important agriculture subject fund from Department of S&T of Zhejiang Province (2015C02044), Department of Education of Zhejiang Province (Y201635576), the Agricultural Technology Extension Funds of Zhejiang University, Dabei Agricultural Discipline Development and Talent Training Fund (2017ZDNT004), and three rural six party funds for Xiaoliang Li.

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  1. Yuanxing Gu and Yingshan Zhou author with equal contribution for this work.

Authors and Affiliations

  1. Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China

    Yuanxing Gu, Yingshan Zhou, Yongping Xin, Ying Shan, Cong Chen, Tong Cao, Weihuan Fang & Xiaoliang Li

  2. Qingdao Agricultural University, Qingdao, 266109, China

    Yuanxing Gu

  3. College of Animal Science and Technology, China-Australia Joint-Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Zhejiang A&F University, Lin’an, 311300, China

    Yingshan Zhou

  4. Animal Products Quality Testing Center of Zhejiang Province, Hangzhou, 310020, China

    Xinfeng Shi

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  1. Yuanxing Gu
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Correspondence to Xiaoliang Li.

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All animal studies were approved by the Animal Care and Use Committee of Zhejiang University in accordance with the Chinese guidelines for the care and use of laboratory animals (Permit Number: 2016101098).

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Handling Editor Zhenhai Chen.

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Gu, Y., Zhou, Y., Shi, X. et al. Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells. Arch Virol 163, 623–632 (2018). https://doi.org/10.1007/s00705-017-3652-2

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  • DOI: https://doi.org/10.1007/s00705-017-3652-2

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