Archives of Virology

, Volume 163, Issue 3, pp 687–694 | Cite as

Molecular characterization of faba bean necrotic yellows viruses in Tunisia

Brief Report

Abstract

Faba bean necrotic yellows virus (FBNYV) (genus Nanovirus; family Nanoviridae) has a genome comprising eight individually encapsidated circular single-stranded DNA components. It has frequently been found infecting faba bean (Vicia faba L.) and chickpea (Cicer arietinum L.) in association with satellite molecules (alphasatellites). Genome sequences of FBNYV from Azerbaijan, Egypt, Iran, Morocco, Spain and Syria have been determined previously and we now report the first five genome sequences of FBNYV and associated alphasatellites from faba bean sampled in Tunisia. In addition, we have determined the genome sequences of two additional FBNYV isolates from chickpea plants sampled in Syria and Iran. All individual FBNYV genome component sequences that were determined here share > 84% nucleotide sequence identity with FBNYV sequences available in public databases, with the DNA-M component displaying the highest degree of diversity. As with other studied nanoviruses, recombination and genome component reassortment occurs frequently both between FBNYV genomes and between genomes of nanoviruses belonging to other species.

Notes

Acknowledgements

This work was supported by funds from ICARDA awarded to SGK. AV and DPM are supported by the National Research Foundation of South Africa.

GenBank accession numbers

KX431380-KX431435, MF510471-MF510474.

Compliance with ethical standards

Disclosure of potential conflicts of interest

The authors declare there are no conflicts of interest.

Research involving human participants and/or animals

The research did not involve human participants or animals.

Informed consent

The research did not involve human participants or animals.

Supplementary material

705_2017_3651_MOESM1_ESM.docx (13 kb)
Supplementary Table 1 List of abutting forward and reverse primers used to isolate full FBNYV genome components and FBNY- associated satellites (DOCX 13 kb)
705_2017_3651_MOESM2_ESM.docx (20 kb)
Supplementary Table 2 List of FBNYV genomes, country of origin, and accession number for each component. Those in bold indicate isolates recovered in this study (DOCX 21 kb)
705_2017_3651_MOESM3_ESM.docx (20 kb)
Supplementary Table 3 List of FBNYV-associated alphasatellites, country of origin, and accession number for each isolate. Those in bold indicate isolates recovered in this study (DOCX 19 kb)
705_2017_3651_MOESM4_ESM.xls (92 kb)
Supplementary Data 1 Pairwise nucleotide sequence identity comparisons of FBNYV component sequences and nanovirus-associated alphasatellite sequences. Names highlighted in red indicate isolates recovered in this study (XLS 92 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  1. 1.The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life sciencesArizona State UniversityTempeUSA
  2. 2.Virology LaboratoryInternational Centre for Agricultural Research in the Dry Areas (ICARDA)TunisTunisia
  3. 3.International Centre for Agricultural Research in the Dry Areas (ICARDA)BeirutLebanon
  4. 4.Laboratory of Plant ProtectionNational Institute of Agronomic Research of TunisiaEl MenzahTunisia
  5. 5.School of Biological SciencesThe University of QueenslandBrisbaneAustralia
  6. 6.Computational Biology Group, Institute of Infectious Diseases and Molecular MedicineUniversity of Cape TownCape TownSouth Africa
  7. 7.Structural Biology Research Unit, Department of Clinical Laboratory SciencesUniversity of Cape TownCape TownSouth Africa

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