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Archives of Virology

, Volume 163, Issue 3, pp 767–770 | Cite as

Completed sequence and corrected annotation of the genome of maize Iranian mosaic virus

  • Abozar Ghorbani
  • Keramatollah Izadpanah
  • Ralf G. Dietzgen
Annotated Sequence Record

Abstract

Maize Iranian mosaic virus (MIMV) is a negative-sense single-stranded RNA virus that is classified in the genus Nucleorhabdovirus, family Rhabdoviridae. The MIMV genome contains six open reading frames (ORFs) that encode in 3΄ to 5΄ order the nucleocapsid protein (N), phosphoprotein (P), putative movement protein (P3), matrix protein (M), glycoprotein (G) and RNA-dependent RNA polymerase (L). In this study, we determined the first complete genome sequence of MIMV using Illumina RNA-Seq and 3′/5′ RACE. MIMV genome (‘Fars’ isolate) is 12,426 nucleotides in length. Unexpectedly, the predicted N gene ORF of this isolate and of four other Iranian isolates is 143 nucleotides shorter than that of the MIMV coding-complete reference isolate ‘Shiraz 1’ (Genbank NC_011542), possibly due to a minor error in the previous sequence. Genetic variability among the N, P, P3 and G ORFs of Iranian MIMV isolates was limited, but highest in the G gene ORF. Phylogenetic analysis of complete nucleorhabdovirus genomes demonstrated a close evolutionary relationship between MIMV, maize mosaic virus and taro vein chlorosis virus.

Notes

Acknowledgements

We thank Dr Kathleen Martin (Kansas State University) for critical reading of the manuscript. This research was jointly supported by the Queensland Department of Agriculture and Fisheries and the University of Queensland through the Queensland Alliance for Agriculture and Food Innovation. AG was supported by a scholarship from Shiraz University, Iran.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2017_3646_MOESM1_ESM.doc (216 kb)
Supplementary material 1 (DOC 217 kb)
705_2017_3646_MOESM2_ESM.docx (55 kb)
Supplementary material 2 (DOCX 55 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  • Abozar Ghorbani
    • 1
    • 2
  • Keramatollah Izadpanah
    • 1
  • Ralf G. Dietzgen
    • 2
  1. 1.Plant Virology Research Center, College of AgricultureShiraz UniversityShirazIran
  2. 2.Queensland Alliance for Agriculture and Food InnovationThe University of QueenslandSt. LuciaAustralia

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