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The expression of HCV-associated host factors is dependent on the hepatoma cell line used in HCV studies

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A Correction to this article was published on 27 September 2018

Abstract

Chronic infection by hepatitis C virus (HCV) is a major cause of liver cirrhosis and hepatocellular carcinoma. At present, the most commonly used in vitro model of HCV infection is based on hepatoma cell lines. However, they were obtained from different patients and different cancers and/or differ in their characteristics and permissiveness to HCV. HCV infection can be modulated by several host factors, so we compared six different hepatoma cell lines that are used as in vitro models for HCV for some of these host factors: the seven known HCV entry factors, the six best-characterized HCV-associated microRNAs, and the two single-nucleotide polymorphisms near the IL28B gene associated with response to pegylated alpha interferon and ribavirin combination therapy, all assessed by quantitative PCR. We showed that the cell lines, including Huh-7 and Huh-7-derived cells, have different microRNA and HCV entry factor expression profiles as well as different IL28B genotypes. In conclusion, some of the observed differences might explain the differences in permissiveness of the cell lines, but, above all, they raise questions about the reliability of in vitro HCV research data gathered to date.

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Acknowledgments

This study was funded by the Conseil Régional de Picardie (Hepanovir, grant no.AAP08-12).

Conflict of interest

All authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. EA4294 Unité de Virologie Clinique et Fondamentale, Université de Picardie Jules Verne, Amiens, France

    Thomas Walter Hoffmann, François Helle, Catherine François, Gilles Duverlie & Sandrine Castelain

  2. Laboratoire de Virologie, Centre de Biologie Humaine, CHU-Hôpital Sud, Centre Hospitalier Universitaire d’Amiens, 80054, Amiens cedex, France

    Thomas Walter Hoffmann, Fabien Delfosse, Catherine François, Gilles Duverlie & Sandrine Castelain

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  1. Thomas Walter Hoffmann
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  2. Fabien Delfosse
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  5. Gilles Duverlie
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  6. Sandrine Castelain
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Correspondence to Sandrine Castelain.

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Hoffmann, T.W., Delfosse, F., Helle, F. et al. The expression of HCV-associated host factors is dependent on the hepatoma cell line used in HCV studies. Arch Virol 159, 527–534 (2014). https://doi.org/10.1007/s00705-013-1862-9

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  • DOI: https://doi.org/10.1007/s00705-013-1862-9

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