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Effects of oseltamivir phosphate (Tamiflu®) in human sera on results of microneutralization and hemagglutinin-inhibition tests for H5N2 avian influenza virus

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Abstract

To determine the influence of oseltamivir phosphate (Tamiflu®) on the results of microneutralization and hemagglutinin-inhibition (HI) tests in human sera with H5N2 influenza virus, ten volunteers were administered Tamiflu® and blood samples were collected. In the microneutralization test, no consistent effects were observed. However, in the HI test, specimens from all volunteers taken at 4 and 7 h after drug administration showed a higher titer as compared to 0 and 24 h after administration when mammalian cells (horse, guinea pig, and human) were used. These results suggest that the administration of Tamiflu® may affect the results of HI tests for H5N2 virus.

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Acknowledgments

We thank the members of the Division of Virology III, National Institute for Infectious Diseases, for kindly providing sera, and the members of the Division of Virology, National Institute of Animal Health, Tsukuba, Ibaraki, Japan, for kindly donating the influenza virus strain.

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Authors and Affiliations

  1. Department of Genetic Science, Ibaraki prefectural Institute for Public Health, 993-2, Kasahara-cho, Mito, Ibaraki, 310-0852, Japan

    Yoshinao Yamazaki

  2. Division of Health and Welfare, Ibaraki prefectural Government, 978-6, Kasahara-cho, Mito, Ibaraki, 310-8555, Japan

    M. Doy

  3. Tsuchiura Public Health Center, 2-7-46, Shimotakatsu, Tsuchiura, Ibaraki, 300-0812, Japan

    S. Yamato

  4. Koga Public Health Center, 6-22, Kitamachi, Koga, Ibaraki, 306-0005, Japan

    Y. Kawada

  5. Chikusei Public Health Center, 114, Koh, Chikusei, Ibaraki, 308-0021, Japan

    T. Ogata

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  1. Yoshinao Yamazaki
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  2. M. Doy
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  3. S. Yamato
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  4. Y. Kawada
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Correspondence to Yoshinao Yamazaki.

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Yamazaki, Y., Doy, M., Yamato, S. et al. Effects of oseltamivir phosphate (Tamiflu®) in human sera on results of microneutralization and hemagglutinin-inhibition tests for H5N2 avian influenza virus. Arch Virol 153, 945–949 (2008). https://doi.org/10.1007/s00705-008-0038-5

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  • DOI: https://doi.org/10.1007/s00705-008-0038-5

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